.

2011-2012

/ : /
:
: 27/11/2011

1
1.
2.
3.
4.
5.
2.
.

ATA

CCC

GCT

GTA

TAT

GGG

CGA

CAT

mRNA

AUA

CCC

GCU

GUA

tRNA

UAU

GGG

CGA

CAU

HOOC

ile

pro

Ser

Met

NH2

. ( 40-41).
....... .
( 40).
..... .
. ( 42).
. .
mRNA ( 36).
. . ( 35).
3
. , cDNA , PCR.
PCR .... .
61.

1 3

 . 2011-2012

. DNA :
,
5 3 , ,
tRNA, rRNA, snRNA.
. DNA :
,
,
DNA ,
.
. , .
64 , 4 ,
43=64 . UGA, UAA, UAG
tRNA
. 3 AUU 5 ,
5 UAA3, .
3ACU5
5UGA3. 3UUU 5 ,
.
4
. . 4-8
DNA ( 57).
B. 133
5GGAUCCGAUGGAAUUCC...385 ...CGAAUGACCGGAUCC 3
, 3
, , , AUG
UGA, UAA, UAG, tRNA ,.
, mRNA AUG
UGA. 399 (10 +385+4) mRNA
, 399:3=133 .
. mRNA- cDNA
mRNA 5GGAUCCGAUGGAAUUCC...385 ....CGAAUGACCGGAUCC 3
cDNA 3 CCTAGGCTACCTTAAGG.385 GCTTACTGGCCTAGG 5

cDNA 3 CCTAGGCTACCTTAAGG385 GCTTACTGGCCTAGG 5

3 CCTAGGCTACCTTAAGG385 GCTTACTGGCCTAGG 5
5 GGATCCGATGGAATTCC..385 ....CGAATGACCGGATCC 3
.
DNA , ,
2 3

 . 2011-2012

,
. amH1

. ( EcoRI
.)
3 CCTAGGCTACCTTAAGG385 GCTTACTGGCCTAGG 5
5 GGATCCGATGGAATTCC...385 ....CGAATGACCGGATCC 3

3 3