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  • ELFOR

    Uploaded by

    Olivia Maria Angelina
    9 views3 pages
    The document contains the results of PCR and restriction analysis including the migration distance of DNA samples and markers, their sizes in base pairs, and logarithmic sizes. Linear regression analysis was performed between the migration distance and logarithmic size, obtaining high correlation coefficients (R^2 = 0.997, 0.979, 0.986) and regression equations to estimate DNA size based on migration distance.

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    The document contains the results of PCR and restriction analysis including the migration distance of DNA samples and markers, their sizes in base pairs, and logarithmic sizes. Linear regression analysis was performed between the migration distance and logarithmic size, obtaining high correlation coefficients (R^2 = 0.997, 0.979, 0.986) and regression equations to estimate DNA size based on migration distance.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as XLSX, PDF, TXT or read online from Scribd
    Flag for inappropriate content
    9 views3 pages

    ELFOR

    Uploaded by

    Olivia Maria Angelina
    The document contains the results of PCR and restriction analysis including the migration distance of DNA samples and markers, their sizes in base pairs, and logarithmic sizes. Linear regression analysis was performed between the migration distance and logarithmic size, obtaining high correlation coefficients (R^2 = 0.997, 0.979, 0.986) and regression equations to estimate DNA size based on migration distance.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as XLSX, PDF, TXT or read online from Scribd
    Flag for inappropriate content
    of 3

    Elfor hasil PCR 1

    Jarak migrasi sample


    5.4
    6.3
    7.6
    7.8
    8.25
    8.65

    ukuran marker
    10000
    8000
    6000
    5000
    4000
    3000
    2000
    1500
    1000
    500

    log ukuran marker


    4
    3.903089987
    3.77815125
    3.698970004
    3.602059991
    3.477121255
    3.301029996
    3.176091259
    3
    2.698970004

    19.65
    -3.949
    Persamaan
    jarak migrasi = -3,682 log ukuran DNA + 18,34

    jarak migrasi marker


    3.8
    4.2
    4.7
    5.1
    5.5
    5.9
    6.7
    7.2
    7.8
    8.9

    log ukuran sample


    3.608508483
    3.380602684
    3.051405419
    3.000759686
    2.886806787
    2.78551532

    0.997

    Regresi

    y = -3.949x + 19.657
    R = 0.9984

    10

    Axis Title

    8
    6
    Regresi

    Linear (Regresi)

    2
    0
    0

    Axis Title

    ukuran sample
    4059.835933
    2402.164166
    1125.655296
    1001.750772
    770.5605777
    610.260584

    Elfor hasil analisa restriksi


    Jarak migrasi sample
    ukuran marker log ukuran marker
    3.5
    10000
    4
    4.3
    8000
    3.903089987
    6000
    3.77815125
    5000
    3.698970004
    4000
    3.602059991
    3000
    3.477121255
    2500
    3.397940009
    2000
    3.301029996
    1500
    3.176091259
    1000
    3
    750
    2.875061263
    500
    2.698970004
    250
    2.397940009
    14.08
    -2.905
    0.979
    Persamaan
    jarak migrasi = -2,905 log ukuran DNA + 14,08

    jarak migrasi marker


    2.8
    3
    3.1
    3.2
    3.5
    3.7
    4.1
    4.3
    4.65
    5.3
    6
    6.4
    7.2

    Regresi
    8
    7

    y = -2.9059x + 14.084
    R = 0.9797

    Axis Title

    6
    5

    Regresi

    Linear (Regresi)

    2
    1

    0
    0

    3
    Axis Title

    log ukuran sample


    3.641996558
    3.366609294

    ukuran sample
    4385.272219
    2325.997772

    Elfor hasil koloni PCR 1


    Jarak migrasi sample
    2.55
    2.8
    3.4
    3.5
    3.7
    4.9

    ukuran marker
    log ukuran marker
    10000
    4
    8000
    3.903089987
    6000
    3.77815125
    5000
    3.698970004
    4000
    3.602059991
    3000
    3.477121255
    2500
    3.397940009
    2000
    3.301029996
    1500
    3.176091259
    1000
    3
    750
    2.875061263
    500
    2.698970004
    250
    2.397940009
    -2.118
    0.986

    jarak migrasi marker


    1.5
    1.6
    1.75
    1.9
    2
    2.3
    2.4
    2.6
    2.9
    3.4
    3.7
    4.1
    4.8

    9.747
    Persamaan
    jarak migrasi = -2,118 log ukuran DNA + 9,747

    Regresi
    6

    Axis Title

    y = -2.1189x + 9.7472
    R = 0.9867
    Regresi

    3
    2

    Linear (Regresi)

    1
    0
    0

    3
    Axis Title

    log ukuran sample


    3.398016997
    3.279981114
    2.996694995
    2.949480642
    2.855051936
    2.288479698

    ukuran sample
    2500.443221
    1905.377859
    992.4188285
    890.1857587
    716.2290565
    194.3030858

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