Nhm 1 Tn thnh vin: Hong Th Ngc nh Bi Hong H L Th Thanh Kim Hoa Phm Th Khnh Ly Bo co k hoch h thng ln men gim

acid acetic 1. Thit k h thng ln men 1.1. Thit b ln men

T 2

H thng ln men c xy dng da trn h thng ln men kt hp ( gia h thng ln men nhanh v h thng ln men chm). Cc thit b ln men ca h thng c nhm thit k v a ra sn phm vo tun 3 va mn hc ( th 2 ngy 5/11). S h thng:

Trong : 1- Thng cha cht mang 2- Thng cha sn phm ln men 3- ng dn mi trng a vo 4- My bm 5- ng dn kh t my bm 6- ng x trn 7- ng dn sn phm i ra

H thng ln men gm cc phn : bnh trn (s 1) l lp m hay lp cht mang cha vi sinh vt, mi trng s c a vo bnh trn bng ng s 3. Bnh di (s 2) cha sn phm sau khi ln men phn trn chy xung v 1 my bm (s 4) thi kh. Kh t my s thi qua phn dung dch chuyn ngc ln phn trn bng ng s 5. ng s 6 c vai tr x bt dung dch t bnh 1 xung bnh 2 khi qu y ( do kh thi). Sn phm ln men s c ly ra bng ng s 7. 1.2. Nguyn, vt liu 1.2.1. Cht mang Yu cu ca cht mang: C din tch tip xc ln Nh, bn, r tin, d kim Khng phn ng vi dung dch ln men, sn phm ln men hay khng kh C nhm, v xp vi sinh vt bm vo

T cc yu cu trn nhm chn b ma lm cht mang. Cht mang s c x l vo tun th 3 ca mn hc, c th l th sng th 3 ( ngy 6/11/2012). Quy trnh x l cht mang: b ma s c ra sch, sau hp kh trng 121C, 1atm trong 30 pht. 1.2.2. Mi trng nui c chun b vo sng th 3 tun th 3 (6/11/2012). Mi trng/ dch ln men phi y cc thnh phn c bn: ngun dinh dng carbon (nc p tri cy, mt ng, r ng, cn ), nito, khong, cc yu t vi lng v cc cht kch thch sinh trng, Bng thnh phn mi trng cy ging v ln men Thnh phn Cn thc phm Nc da Nc go Gim n ng glucose Nc ct/ nc my Dung dch mi trng ( khi cha b sung dch ging) th c em kh trng. Hai mi trng c kh trng 121C, trong 20 pht. Trc khi ln men th mi b sung ging vo mi trng kh trng. Mi trng c mang i hp kh trng 121C, 1atm trong 15-20 pht. S lng

1.2.3. Cc ha cht v dng c khc cn thit c chun b vo th 3 ( ngy 6/10/2012) - My o pH - Dung dch chun : NaOH 0.1N, HCl 0.1N v phenolphthalein STT Dng c Ghi ch 1 2 3 Erlen 250 ml Pippet 10 ml Bp cao su

1.2.4. Ging vi sinh vt Ging c la chn l Acetobacter, going s c ly t mu gim nui c mua ch. Ngy mua ging cng l th 3 ( 6/11/2012) 2. Cch tin hnh Sau khi chun b xong h thng v cc vt liu th bt u chy h thng ln men ( th 4 ngy 7/11/2012) H thng ln men

Kh trng bng cn

Kh trng bng cn

Cho b ma vo thng s 1

B sung gim nui vo bnh 1 Sau ngy My bm hot ng Rt dch gim ra, b sung nc da, ng, ru

Thu sn phm, kim tra cht lng