You are on page 1of 2

Gel Electrophoresis

Name: Date: Go to the virtual gel electrophoresis lab 1. What goes into the wells at the end of the slab of gel? 2. Why does the DNA move through the gel? 3. How does the gel electrophoresis sort out the DNA strands into separate lengths? 4. If the DNA is so small, what has to be done so that we can see the strands after they have been separated? 5. List the ingredients and equipment required to make the gel.

6. Write a method for making the gel

7. What is the purpose of a gel comb? 8. What equipment is needed to load DNA samples into the gel? 9. What is loading buffer used for? 10. Why do we include a DNA size standard? 11. Which colour electrical cord should be placed closest to the wells? Why?

12. What should you notice in the electrophoresis box that would show that the current is running?

13. What is ethidium bromide used for? When should it be applied? How long does it take to work?

14. How do you get to see the DNA bands once you have added ethidium bromide?

You might also like