Bi 4: XC NH URE TRONG THC PHM

I. Chun b mu Mu em phn tch l mu Ure trong bnh c k hiu mu A II. Phng php tin hnh - Pha long dung dch Ure 1%: Ht 1ml dung dch Ure 1% cho vo bnh nh mc 100ml, thm nc ct vo ti vch 100 dung dch Ure 0,01% ng nghim Hm lng Ure (g/ml) Dung dch Ure 0,01% Nc ct 1 0 2 10 3 20 4 30 5 40 6 50

0 10

1 9

2 8

3 7

4 6

5 5

Lc u ly lot ng nghim khc v thc hin theo bng di y: ng nghim Hm lng Ure (g/ml) Nc ct (ml) Dung dch Ure chun Dung dch mu A Phenol Red (git) Urease (ml) - Lc u III. Kt qu 2 1 2 1 2 1 2 1 2 1 2 1

1 0 3 1

2 10 3 1

3 20 3 1

4 30 3 1

5 40 3 1

6 50 3 1

7 3 1 2 1

8 3 1 2 1

Sau 30-45 pht quan st, so snh mu vi cc ng nghim dy chun, mu phn tch c kt qu dng tnh v c mu gn vi mu ca ng nghim s 4 trong dy chun. Vy mu phn tch c hm lng ure l 30 g/ml IV. Gii thch kt qu Urease thuc nhm protease l enzyme thy phn c hiu Ure to NH3 trong mi trng kim.Ch th phenol red rt nhy vi s thay i pH ca mi trng, khi lng NH3 to ra cng nhiu th s thay i mu ny cng ln. Trong th nghim trn, khi cho Urease vo cc ng nghim, lc u, sau yn quan st trong khong 30 pht thy c s chuyn mu gia cc ng nghim, t mu vng cam sang mu hng.S chuyn mu ny l do urease tc dng vi Ure to NH3, ng nghim 5, 6 mu m hn l do hm lng Ure trong ng cao hn so vi cc ng cn li. Mu cn phn tch c so mu vi cc ng nghim chun th thy mu gn ging vi ng s 4.Da vo dy chun c th kt lun hm lng Ure trong mu cn phn tch l 30 g/ml.