Day 1: Thursday 1pm 1.

Procurement of samples - squid balls, siomai and kwek kwek from CHE (Neefa), Vinzons(Carl), and SC (Shu) - total of 9 samples 2. Preparation of incubating media - By Shela while samples are being bought - LB broth = 9 mL per sample with 9 samples so 81 mL ~ 100 mL - 0.9% saline solution = 50 mL per sample with 9 samples so 450 mL ~ 500 mL 3. Homogenize 5 g sample in 50 mL 0.9% NaCl 4. 1 mL aliquot and add to 9 mL of LB broth in a falcon tube 5. Incubate overnight 6. Media preparation* - SMAC for E.coli by Neefa = 9 samples, 3 replicates so 27 plates ~ 1 L - Salmonella-Shigella for Salmonella by Carl = 9 samples, 3 replicates so 27 plates ~ 1 L - MSA for Staphylococcus by Shu = 9 samples, 3 replicates so 27 plates ~ 1L - LB broth for optimization and metagenome culture by Shela = 9 samples, 4 dilutions and the 9 samples for lawn plate so 45 ~ 1.5 L - Malamang may excess so use for re-streaking. One sample, one selective medium, one plate for 3 replicates tapos ihiwalay nalang 7. Pour plating of autoclaved media and storage *pwede rin na 2 dilutions yung iplate natin in which case, times two lahat