Tp ch Nghin cu Khoa hc 2008: 8 149 - 157

Trng i hc Cn Th

PHN LP CC DNG VI KHUN NI SINH SN XUT PHN VI SINH QUY M PHNG TH NGHIM CHO CY MA TRNG TI TNH SC TRNG
Nguyn Hu Hip 1 , Renato Fani 2 , L NgcThy 3 , Ng Bo Ngc 4 , Trn th NgcT4 v Phm th Khnh Vn1
Email: nhhiep@ctu.edu.vn

ABSTRACT
Twenty six endophytic isolates from roots, stems and leaves of twelve sugarcane cultivars grown in fields in Cu Lao Dung district and My Tu district of Soc Trang province were isolated. With PCR technique using specific primers of nifH gene of Gluconacetobacter diazotrophicus, we could identify fifteen strains. These strains have the same characteristics with the G. diazotrophicus species. Molass is a good carbon source for G. diazotrophicus especially at the concentration of 10g/l, G. diazotrophicus reached 6,7 x 1010 CFU/ml after 6 days of incubation. Carriers containing of 50% of peat, 25% of filtercake, 25% of bagasse and 8% CaCO3 supported the good survival of G. diazotrophicus. After two months of storing at room temperature the viable count was still high 5,3x109 CFU/g. Keywords: Endophytic bacteria, sugarcane, PCR technique, primer, Gluconacetobacter diazotrophicus Title: Isolation of endophytes for the production of biofertilizer at lab scale for the cultivation of sugarcane in Soc Trang province

TM TT
Hai mi su dng vi khun ni sinh c phn lp t l, thn v r ca mi hai ging ma trng ti huyn C Lao Dung v huyn M T, Tnh Sc Trng. S dng k thut PCR vi cp mi c hiu thit k da trn trnh t gen nif H ca Gluconacetobacter diazotrophicus, chng ti nhn din c mi lm dng vi khun G. diazotrophicus. Cc dng c cc c im hon ton ph hp vi nhng nghin cu trc y v vi khun G. diazotrophicus. Ngun carbon thch hp cho s tng trng ca vi khun G. diazotrophicus l r ng (10g/l, 5g/l, 2,5g/l), c bit l nghim thc r ng 10g/l cho mt s cao nht (6,7 x 1010 CFU/ml) sau 6 ngy nn c chn l ngun carbon s dng trong mi trng nui vi khun thu sinh khi. Thnh phn cht mang 50% than bn + 25% b bn ma + 25% xc ma + 8% CaCO3 gip vi khun sng st cao nht, mt s vi khun t 5,3 x 109 CFU/g cht kh khi bo qun sn phm trong 2 thng nhit phng (28-300C). T kho: vi khun ni sinh, ma, k thut PCR, mi, Gluconacetobacter diazotrophicus

1 2

Vin Nghin cu v Pht trin Cng ngh Sinh hc, i hc Cn Th. B mn Sinh hc ng vt v Di truyn, i hc Florence, . 3 Sinh vin Cng ngh Sinh hc K 27 4 Sinh vin Cng ngh Sinh hc K 28

149