Ethanol precipitation of RNA. Add: 0.5 vols 7.5M Ammonium acetate 2.

.5 vols ice cold 100% Ethanol (Analar) Vortex to mix thoroughly. Precipitate at -20 0C for 1 hour or overnight. Centrifuge at full speed (13000rpm), 40C for 30 mins. Wash pellet twice with 0.5ml ice cold 80% Ethanol, spinning at 40C for 10 mins each time. Air dry the pellet and resuspend in an appropriate volume of Nuclease free water. Precipitating small amounts of RNA Linear Acrylamide may be added to the RNA before precipitation to aid visualization when precipitating small amounts of RNA. Add 2ul of a 5mg/ml solution of Linear Acrylamide

Reagents: 7.5M Ammonium Acetate Sigma A2706 Nuclease Free Water Ambion 9937 Linear Acrylamide Ambion 9520