, 2012, 81, 5, .

665671

576.8.093.1.095.21

2012 . . . , . . , . . 1
. .. ,
24.01.2012 .

, , 
,
0.22 . [1],
Herbaspirillum, Herminiimonas, Curvibacter Burkholderia Be!
taproteobacteria, LDS.

, .

,  ,
[1].
3.8 106 0.5% .
100 16S 77
16S . 16S 
Beta Gammaproteobacteria ( Janthinobacterium
Pseudomonas ), Bacteroidetes ( Chryseobacterium Epilithonimonas)
,
. , .
LDS, Meth!
anobacteriales Methanosarcinales. ,
Methanomicrobiales,
RCV (Rice Cluster V), Thaumarchaeota.
: , ,
16S .
1



,

[2]. 
, 
, 

, 104
,
0.22 [1]. 

, ,
.

Herbaspirillum, Herminiimonas, Curvibacter
Burkholderia Betaproteobacteria.

Alpha Gammaproteobacteria,
Actinobacteria. 
1 (email: dedysh@mail.ru).

Eur
yarchaeota,
, 
,
LDS (Lake Dagow Sediment) [3, 4].
16S


[4],
, 

.
, ,

,
[57]. , 
 80%
, ,
0.5 [5].
,

in situ (FISH)

665

666

,
Bacteria. ,
0.2 ,

,
16S , [8].


 
Archaea, 
.
[6], ,

0.2 , 
15 108 1 
. FISH

, 
Archaea,
Proteobacteria,
Bacteroidetes Actinobacteria [6].


.
,

, 
, 
. 

, 


, 
,
[1].

. 
, 5
15 (
., 5814 N, 3812 E) 2010 . 


. 
Sphagnum angusti!
folium, Sph. fuscum, Carex spp., Oxicoccus sp. Vac!
cinium sp. 3 . 
4.2.

5 .
:
. 
1 ( 97%) , 


MiniMix ( 100, Interscience, )

BagFilter. , 
. 


(1 ). 1
, 20 
, 10
1 , 
, . 

1 : 1 20.

15 ,
0.3
(Millipore, 0.22 ) 
.


0.22 ,
0.09 . 
4 ,
0.5 
(4',6'
2) 10 , 
.

Zeiss Axioplan 2
(Carl Zeiss, ) Zeiss
02, .
100 
1
. 
,
0.22 ,
.

. 

2 20 
, Mini
mix , 
5 (
)
.


0.22 

0.1 . 
2
. 

FastDNA SPIN kit for soil (Biol 101, )
.


().


(1490 ) 16S 

81

2012

9f 1492r [9]. 
(800 ) 
16S
109f 915r [10].
PE GeneAmp PCR System 9700
(PerkinElmer Applied Biosystems, ). 

1.2% 


.
pGemT
Easy Vector SystemII (Promega, ).


 7 SP6. 

.

MspI RsaI, HhaI
BsuRI (HaeIII). 
2.5% .

Wizard Plus
Minipreps DNA Purification System (Promega,
). 
ABI 377A
(PerkinElmer Applied Biosystems, ). 


SeqMan (Laser Gene 7.0; DNA Star Package).

GenBank 
Blast2 (http://
www.ebi.ac.uk/Tools/blast2/).
16S 
Belle
rophon 3.0 (http://compbio.anu.edu.au/bellerophon/
bellerophon.pl).

ARB (http://www.arbhome.de).


Phylip bootstrap 
1000 .

16S 

GenBank JQ697092JQ697150.

.
,
1 
, 8.06 0.86 108 .
0.22
3.75 0.42 106 1, 0.5%

81

2012

667

. 


[6] 
1 .

.

16S 
100 , 65 

Proteobacteria 35 
Bacteroidetes (. 1).
Betaproteobacteria
(44 ) Gammaproteobacteria (21 ), 
Bacteroidetes Flavobacteria (34 
) Sphingobacteria (1 ).

16S , Betaproteobacteria, 
(9899%) 
Janthinobacterium,
J. lividum, 
, 
, 
[11].
Gammaproteobacteria
21 ,
(99%)
16S
Pseudomonas Ps. psychrophila, Ps. antarctica
.

Acinetobacter (99%
) , 

[12].

16S 

Flavobacteria (. 1). 10 
Chryseobacterium
24 Epilithonimonas.
(99%) 
16S Sphingobacterium
faecium, .
,

. ,


, 
Janthinobac!
terium,
Herbaspirillum, Herminiimonas, Curvibacter
Burkholderia [1]. 
, .

Bacteroidetes

668

100

100

100

Clone POB 14 (8)

Clone POB 36 (22)
Clone
POB 38 (6)
Betaproteobacteria
100 Janthinobacerium
lividum, AF174648
73
Clone POB 23 (8)
Janthinobacerium sp., GU733463
Clone POB 21
94
Clone POB 50
99
Clone POB 106 (2)
Clone POB 11 (7)
Pseudomonas antarctica, FM955872
Gammaproteobacteria
100
Clone POB 7 (9)
Pseudomonas fluorescens, AF506040
100
Acinetobacter lwoffii, AF188302
Clone POB 54
Chryseobacterium balustinum, M58771
100
Clone POB 25 (9)
Chryseobacterium scophthalmum, AJ271009
Chryseobacterium vrystaatense, AJ871398
Clone POB 110
100
Chryseobacterium formosense, NR_036872
98
Bacteroidetes
Clone POB 15 (12)
100
Clone POB 17 (12)
lactis, EF204460
100 Clone POBEpilithonimonas
34
Sphingobacterium faecium, AJ438176

0.10

. 1. , 
16S , ,
Proteobacteria Bacteroidetes. , 
, POB.
16S Gemmata obscuriglobus (X54522), Isosphaera pallida (AJ231195), Schlesneria paludicola
(AM162407) Planctomyces limnophilus (X62911). 0.1 .

. 
[6],
25% 
, 
FISH.

. 
,
FISH [6], 

. 
,


[1315], Acti!
nobacteria
.
,

, .
, ,

( Janthinobacterium, Pseudomonas
Epilithonimonas) 

.
, , 
[1618]. 
, 
Janthinobacterium
.
, 


, .

( ~ 0.08 2)
Chryseobacte!
rium,
Chry!
seobacterium greenlandense [19].

Chryseobacterium,
[20].
, 

,
,

, ,

.
, , 
.

. 16S
77 
,
73
Euryarchaeota 4 Thaumarchaeota.
16S 

Methanobacteriales, Meth!
anomicrobiales Methanosarcinales. 
(41 )
16S , 

81

2012

100
98
100

100

69

Clone POA 7, POA 19, POA 34, POA 79, POA 81

POA 97, POA 112, acidic lake archaea JN825226
100
and JN825285
Clone POA 77
98
Mackenzie river archaeon, EF014515
acidic lake archaeon, JN825289
100 Clone POA 110 (2)
98
acidic lake archaeon, JN825241
100
Clone POA 40, POA 62, POA 96, acidic lake
archaea JN825247 and JN825286
acidic lake archaeon, JN825286
Clone POA 89
100 acidic
lake archaeon, JN825238
acidic peat archaeon, AM712544
100
acidic lake archaeon, EU782019
97 Clone POA 6, POA 9 (3), POA 29 (3), POA 55
acidic lake archaeon, JN825228
100
acidic lake archaeon, JN825281
Lake Llebreta archaeon, FR820727
60
boreal fen archaeon, AM905420
96
Clone POA 117
Clone POA 32
Clone POA 132
84
100
Clone POA 42
Clone POA 60
Clone POA 1 (6), POA 21 (2), POA 43, POA 47, POA 51 (2),
POA 57 (2), POA 58 (3), POA 67 (2), POA 84 (2), POA 93 (2),
POA 98 (4), POA 122 (3), POA 133 (7)
Clone POA 37 (2)
100 Clone
POA 45 (2)
97
Methanoregula boonei, DQ282124
Methanosphaerula palustris, EU156000
Clone POA 109
Clone POA 59 (2)
100
Methanosphaerula lacustera, AF432127
Clone POA 73
boreal fen archaeon, AM905408
peat bog archaeon, FJ822548
100
Clone POA 139
Methanosphaerula subterraneum, X99045
wetland soil archaeon, AB570044
boreal fen archaeon, AM905394
peat bog archaeon, FJ822579
Clone POA 2
98 Clone
POA 15
100
Clone POA 131 (2)
boreal forest archaeon, GU815337
Candidatus Nitrososphaera gargensis, EU281336
Cenarchaeum symbiosum, U51469
Nitrosopumilus maritimus, DQ085097

669

LDS cluster

RC:V

Methanomicrobiales

Methanosarcinales
Methanobacteriales

Thaumarchaeota

0.10
. 2. , 
16S , , 
, , 
. , , 
POA. 16S Gemmata
obscuriglobus (X54522), Isosphaera pallida (AJ231195), Schlesneria paludicola (AM162407) Planctomyces limnophilus
(X62911). 0.1 .

Methanomicrobiales 
9798% 
Methanoregula boonei [21,
22].

0.20.3 .
, Methanobacteriales,
Methano!
bacterium subterraneum,
(0.100.15 )
[23]. Methanosarcinales
[1],
.

16S 

81

2012

Euryarchaeota LDS (Lake
Dagow Sediment) (22 ) RCV (Rice Cluster V)
(5 ) (. 2). 
16S 
75%.
, 
RCV, 1998 .
[24]. 
LDS , 2004 .,
,
[3]. 2008 . LDS RCV 
370 500 
[4]. 
,

670

, , 


[4].

.
, 
LDS .

LDS, , 

,

[1] (. 2).

16S ,
, [2527],
[28].

16S
Thaumarcha!
eota [29, 30].

,

. 
Candidatus Nitrosopumilus ma
ritimus,
0.170.22 [31].
, ,
( 99%)
, 
[28, 32, 33]. ,
Thaumarchaeota 
.
,
, 
0.22 ,

. 
, 

Euryarchaeota,
, 
.

.




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