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  • Development and Testing of Virus Induced Gene Silencing (VIGS) Vector For Banana (Musa Acuminata)

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    Nurulzahidah Arshad
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    This document summarizes experiments testing virus-induced gene silencing (VIGS) vectors for banana plants using cucumber mosaic virus. It describes inoculating 10 banana plantlets that were 7 weeks old and 5 cm in height with the virus using carborundum tips as a mechanical inoculation method. After 7 and 30 days, it records the results of inoculation using carborundum or by scratching with tips. Finally, it provides status updates for different batches of inoculated and non-inoculated plants of varying ages and proposes next steps like finding a new inoculation site and practicing different inoculation methods.

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    This document summarizes experiments testing virus-induced gene silencing (VIGS) vectors for banana plants using cucumber mosaic virus. It describes inoculating 10 banana plantlets that were 7 weeks old and 5 cm in height with the virus using carborundum tips as a mechanical inoculation method. After 7 and 30 days, it records the results of inoculation using carborundum or by scratching with tips. Finally, it provides status updates for different batches of inoculated and non-inoculated plants of varying ages and proposes next steps like finding a new inoculation site and practicing different inoculation methods.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as PPTX, PDF, TXT or read online from Scribd
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    14 views8 pages

    Development and Testing of Virus Induced Gene Silencing (VIGS) Vector For Banana (Musa Acuminata)

    Uploaded by

    Nurulzahidah Arshad
    This document summarizes experiments testing virus-induced gene silencing (VIGS) vectors for banana plants using cucumber mosaic virus. It describes inoculating 10 banana plantlets that were 7 weeks old and 5 cm in height with the virus using carborundum tips as a mechanical inoculation method. After 7 and 30 days, it records the results of inoculation using carborundum or by scratching with tips. Finally, it provides status updates for different batches of inoculated and non-inoculated plants of varying ages and proposes next steps like finding a new inoculation site and practicing different inoculation methods.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as PPTX, PDF, TXT or read online from Scribd
    Flag for inappropriate content
    of 8

    Development and Testing of Virus Induced Gene Silencing (VIGS) Vector for Banana (Musa acuminata)

    Nurulzahidah Binti Arshad SGF110021 Supervisor: Prof. Dr. Jennifer Ann Harikrishna Prof. Dr. Rofina Yasmin Othman

    Virus Host Test: Batch 3 Virus : CMV Method : Mechanical Inoculation Carborundum Tips Buffer : Phosphate buffer 1.0 M, ph 7.5 Number of sample : 10 plantlets Age / Height : 7 weeks / 5 cm

    2nd day of inoculation

    Method: carborundum

    Method: scratch using tip

    7th day of inoculation

    Method: carborundum

    Method: scratch using tip

    After 30th Day of Inoculation

    No of plants (Batch 1) 6 8

    Age/Height 6 months/ 20cm 6 months/ 20cm

    Current Status
    Inoculated (-ve result) (Carborundum/ Syringe)

    Not inoculated

    (Batch 2) 7 5
    (Batch 3) 9 3 10 10

    3 months/ 8-10cm
    3 months/ 8-10cm 7weeks/ 5cm 7weeks/ 5cm 2 weeks 1 week

    Inoculated ( Syringe) Not inoculated


    Inoculated (Carborundum) Not inoculated Not inoculated Not inoculated

    -Find new place for inoculation (C6) -Alingment of several Cucumber Mosaic Virus RNA 2 strain, Alingment of banana PDS gene with other monocoat plant. - Practise inoculation (injection/rubbing/Vacum inflitration) method

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