Preliminary Phylogenetic Study of Terubok Fishes (Genus Tenualosa: Clupeidae) Inferred From Sequencing of Cytochrome b Mitochondrial DNA Fragment

Nadiah Binti Halim1, Khairul Adha A. Rahim2 & Yuzine B. Esa*

1Department

of Zoology & 2Department of Aquatic Science Faculty of Resource Science and Technology Universiti Malaysia Sarawak * Corresponding author: eyuzine@frst.unimas.my

ABSTRACT This preliminary study examines the phylogenetic relationship among three species of Terubok; Tenualosa toli, Tenualosa macrura and Tenualosa ilisha. A total of 15 samples comprising of ten T. toli, two T. macrura and three T. ilisha mitochondrial Cytochrome b (Cyt b) sequences were analysed.. Phylogenetic results matched species identification based on morphology. All phylogenetic methods (NJ, MP, ML and Bayesian) supported the reciprocally monophyletic status between the three Teruboks with high genetic distances (13.943-16.033%) between pairwise comparisons. Overall, this preliminary study has managed to provide useful informations on the phylogenetic status of terubok in Sarawak. Keywords : Tenualosa, Terubok, phylogenetic relationships, Cyt b sequences

T. toli

T. macrura

T. ilisha Outgroup

T.toli Sampling locations of T. toli and T. macrura in Sarawak

T. ilisha T. macrura Phylogenetic relationships between the three terubok species represented by a Bayesian tree

Pair-wise genetic distance between terubok species

Acknowledgements We thanked all the staff and personnel from UNIMAS and others who were involved in this project. This project was partially funded by FRGS/01(10)707/2009(23)