-VE TEST for

Vitamin A
- Colourless
- Clear fluid
- So, I guess
Based on the fact that
concentrated sulphuric acid
Takes up H20 from retinol
and yields colored product
(blue)
Since it is a fat soluble vitamin,
we shall dissolve it with
chloroform.
Add 2 drops of fish oil into a test tube
Add 1-2 ml of chloroform
Lastly, add 1-2 drops of concentrated sulphuric acid,
and blue coloration will be form.
Vitamin A
Vitamin A exist in 3 biological active forms ;
Retinol
Retinal
Retinoic acid

Vitamin A is derived from the provitamin
carotenes by carotenase enzymes which occur
in the interstinal mucosa and liver.
Vitamin A
Carotenes = dark green yellow vegetables and
fruits ; carrots, sweet potatoes ,spinach,
apricots and green leafy vegetables

Performed vitamin A from animal sources ;
liver, egg yolk, butter, whole milk and fish liver
oil

Vitamin A
Uric Acid
It is the result of purine catabolism.
Principle :
Uric acid + O2 + 2 H2O ------> Allantoine +
H2O2 + CO2

2 H2O2 + DCPS + 4- Amino phenazone ------>
Red Quinonimine + 4 H2O

Uric Acid

Procedure :
1. Prepare two test tube labelled sample and standard
2. Add 1.0ml of the reagent into both tubes
3. Add 50 ul of standard (uric acid) in test tube labelled
standard and 50 ul of sample in test tube labelled
sample.
4. Shake both test tube and leave it for 10 minutes in
room temperature.
5. Calculate the absorbance of sample and standard.

Dont Forget to do
several wash
Calculate the
absorbance
of sample
and standard
Jangan Lupa bawak kalkulator
(susah nak pinjam2 member)
- Pak kura
Mutant forms of PRPP
synthetase
Partial HGPRtase deficiency
Abnormalities
in purine catabolism
Abnormalities in other
metabolic pathway
Deficiency in G-6-
phosphatase ( Von
Gerk's disease)
Elevation of
glutathione
reductase activity
Increase
consumption of
meat
Malignancy and
chemotherapy
Kidney diseases,
decrease uric acid
excretion
Decrease
Uric Acid
Production
Inhibit Xanthine
Oxidase
Anti-
inflammatory
Uricosuric
Drugs
:| s ..! _ _|| ,, s! _
Maka, apabila kamu telah selesai (dari sauatu urusan),kerjakanlah
dengan sungguh-sungguh (urusan) yang lain. Hanya kepada
Tuhanmu hendaknya kamu berharap.

( Alam Nasyrah ayat 7-8 )