Cholesterol Assay Kit CHO

CHOD PAP Cat. 0182~0185 Size 50ml4; 60ml4; 80ml4; 100ml2

Intended Use: For the quantitative determination of cholesterol in human serum. Principle: Cholesterol ester +H2O Cholesterol + O2
CHOD CHE

Dissolve one bottle of R1 with a ration of R2 (refer to label on R1) as working reagent. Blank Calibrator Samp le Cholesterol +Free fatty Working reagent Blank 0.01ml 0.01ml 0.01ml 1.0ml 1.0ml 1.0ml

Cholestenone+ H2O2
POD

2H2O2 + 4-aminoantipyrine + 4-chlorophenol

Calibrator Sample

quinoneimine + 4H2O Contents: Reagen t R1 Cholesterol esterase (CHE) Cholesterol oxidase(CHOD) Peroxidase (POD) R2 PBS pH7.7 4-chlorophenol 4-aminoantipyirne Calibrator, refer to the label Reagent stability: 12 months at 2 8, protecting from light. 8 and 24 hours 1140U/L 800U/L 6000U/L 100mmol/L 3.5mmol/L 0.5mmol/L Component Concentration Mix well

incubate at 37 for 6 minutes. Adjust zero by blank

reagent, then read the absorbance of standard (Astandard) and sample (Asample) respectively. Calculation: Asample Cholesterol= standard(mmol/L or mg/dl) Astandard Reference values: Adult: <5.17mmol/L 200mg/dl Critical value: 5.17-6.47mmol/L 200-250mg/dl

Hypercholesterolmia: >6.47mmol/L 250mg/dl The reference range should be determined by each hospital to confirm with the characteristics of the region being tested. Linearity:

Dissolved reagent is stable for 2 weeks at 2 at room temperature, protecting from light. Sample: Serum Procedure: Wavelength: 500nm(480nm~520nm) Reactive Temperature: 37 Cuvette light path: 1cm Heparin or EDTA plasma.

The test is linear up to 12. 93 mmol/L (500mg /dl). Notice: 1. If the cholesterol is very high, dilute the sample with 0.9% NaCl and re-assay it.

2.

For

in

National Clinical Diagnose Operation Program 2 nd Edition, Yingwu Ye & Shusan Wang. 1997, P270.

vitro diagnosis! NaN3 included. Do not contact with skin or swallow, wash skin with plenty of water in case of contacting with skin. 3. Shall be stocked 2~8. Avoid temperatures over

Registration number SFDA-3400195 (2004) Standard number YZB/GB 2084-2003

2~8 or lower than 0. Reference: 1.

Manufacturer Biosino Bio-Technology and Science Inc. AddNo.27, Chaoqian Road, Changping District, 102200 Beijing, China Tel+86-10-69722840Fax+86-10-69722840 Website www.zhongsheng.com.cn