Lead (Pb) Bioaccumulation In Genera Bacillus Name : Achmad Arifiyanto NRP : 1509 100 062 Department : Biologi Advisor

Lecturer : Dr.rer.nat.Ir.Maya Shovitri, M.Si. ABSTRACT Lead (Pb) includes a group of large heavy metal in nature were toxic either on animal or human and did not provide an advantage functional biologically. Bacillus isolates S1 and SS19 known resistant to lead up to 50 mg / L PbCl2. In this research will be examined whether genera bacillus isolates S1 and SS19 can accumulate metal lead (Pb), their capability in accumulating and profile protein differences when the bacteria genera bacillus isolates S1 and SS19 get exposed metal lead (Pb). Inoculum at age 9 hours are used, with a Nutrient Broth (NB) containing 50, 75 and 100 mg / L PbCl 2. Inductively Coupled Plasma Atomic Emission Spectrometry (ICP) used to assessed Pb2+ concentrations. Bioaccumulation levels of Pb2+ by Bacillus isolate S1 and SS19 related to the distinction of beginning concentration to the final concentration. Bacillus isolate S1 achieved 53% and 51% bioaccumulation efficiency rate in lead presence concentration (75 and 100 mg/L) and 51% (50 mg/L). Another way Bacillus isolate SS19 able to accumulate 57% (50 mg/L PbCl2) and kept stable on 36% bioaccumulation efficiency rate (75 and 100 mg/L PbCl2). Regarding SDSPAGE electrophoresis protein profile result, protein in 127 kDa, molecule mass detected in the presence of Lead for Bacillus isolate S1. Key words : Lead, Bacillus isolate SS19, Bacillus isolate S1 and bioaccumulation iii