61

TAP CH KHOA HOC V CNG NGHE Tp 44, s 2, 2006 Tr. 61-64

NGHIN CU IN VIVO TC DUNG NQI TIT KIEU OESTROGEN
CA ISOFLAVONES CHIT XUT T SAN DY PUERARIA
THOMSONII BENTH.
TH| HOA VIN
I. M U
Sn dy l mt loi cy thuc lu i Vit Nam v c trng kh ph bin t vng min
ni n ng bng. T lu, y hc dn gian coi sn dy nh mt loi thuc c th cha c
nhiu chng bnh nh cm st phong nhit, kit l km theo st, gii nhit,... Mt trong s cc
nhm cht c chng minh l c tc dng cha bnh ca sn dy l cc dn cht thuc nhm
isoflavonoid c hot tnh oestrogen, hay cn gi l cc phytoestrogen. y l mt nhm hot
cht a dng c ngun gc t thc vt m nhng cht ny c cu trc v chc nng tng t nh
hoocmon estrogen ca ng vt c v, c kh nng thay th estrogen trong c th ph n. Cc
phytoestrogen c tc dng ngn nga cc biu hin ri lon hoocmon cng nh lm gim nguy
c mc cc bnh v tim mch, long xng, ung th v, ... ph n thi k tin mn kinh v cn
c tc dng phng chng ung th tin lit tuyn nam gii.
V vy, nhiu nghin cu xem phytoestrogen nh mt s la chn cho gii php thay th
hoocmon (hormone replacement therapy- HRT) v gii php thay th estrogen (estrogen
replacement therapy- ERT).
Vit Nam, sn dy c 5 loi, ph bin nht l loi Pueraria thomsonii Benth. c trng
lm thc phm v lm thuc. gp phn nghin cu v khai thc cc hot cht c hot tnh
sinh hc t sn dy, chng ti tin hnh nghin cu chit xut cc phytoestrogen t sn dy
v th nghimhot tnh ca chng trn ng vt.
II. NGUYN LIU V PHNG PHP NGHIN CU
1. Nguyn liu v i tng nghin cu
Nguyn liu: Cao chit isoflavone t c sn dy Pueraria thomsonii Benth, huyn Lm
Sn, tnh Ho Bnh.
Ni dung nghin cu: Tc dng ni tit kiu oestrogen ca isoflavone chit xut t sn dy
trn chut nht trng.
i tng th nghim: Chn chut nht trng ci kho, nhanh nhn, lng mt, mt
hng, ui hng r tnh mch c trng lng t 14 - 17 g.
Dng c: Knh hin vi soi t bo, lam knh, que ly t bo, kim tim, bn m, pince, ko,
giy o pH, bng,...
Ho cht: Dung dch chun EDP (Estradiol dipropionat), thuc nhum dim sa, cn 90
o
,
nc ct,...
2. Phng php nghin cu
Tin hnh theo phng php Allen- Doisy:
62
Bc 1: C nh chut nmsp trn bn m.
Bc 2: Ct b hai bung trng, th thut c tin hnh vng hng, ct xong bung
trng tin hnh khu 2 lp, lp c ri n lp da.
Bc 3: Sau khi ct b hai bung trng cho chut ngh 15 ngy. n ngy th 16 kim tra
loi b nhng con thin st.
Bc 4: Chut khng thin st c chia thnh 3 l th nghimsau:
+ L chun: Tim EDP vi liu 0,05mg/con (tim mt ln ti bp i).
+ L chng: ung nc un si ngui 0,5 ml / con / ngy.
+ L th: Chut c ung cao chit vi liu 150 mg / 0,5 ml nc ct / con / ngy.
L chng v l th chut c ung lin trong 7 ngy.
Ngy no cng kimtra t bo m o, pH m o theo di s xut hin cc t bo sng.
III. KT QU V THO LUN
1. Ch tiu quan st
- T bo m o: Tiu bn dng tnh: l nhng t bo sng ho trn ton b tiu bn; Tiu
bn m tnh: khng c t bo sng, ch c bch cu v mt t t bo nhn trn tiu bn.
- pH m o: theo di s bin i pH m o di tc dng ca isoflavone chit xut t sn dy.
2. Kt qu th nghim
Bng 1. Kt qa th nghimin vivo tc dng ni tit kiu estrogen ca cao chit
isoflavone t c sn dy Pueraria thomsonii Benth.
pH m o
Trc Sau
Ngy c t
bo sng
S con c
t bo sng
T l % c t
bo sng
Ghi ch
L chng
(ung nc un
si ngui)
6,6 6,8 4 1/27 3,7%
T bo sng xut hin
rt t vo ngy th 4,
ch c t bo nhn v
bch cu.
L chun
(tim EDP)
6,8 7,0 1 28/28 100%
C t bo sng t
ngy th 17.
L th
(ung cao chit)
6,7 6,9 4 17/33 51,5%
C t bo sng t
ngy th 47.
( : iu tr)
Nhn xt:
- l chut c tim dung dch chun EDP, kim tra t bo m o cho thy: cc t bo
sng xut hin t ngy th nht n ngy th 7 trn 100% s chut th nghim.
- l chut ch ung nc, hu nh khng c t bo sng, ch c t bo nhn v bch cu.
Cc tiu bn c xem l m tnh.
- l chut cho ung cao chit, cc t bo sng xut hin t ngy th 4 n ngy th 7 trn
51,5% s chut th nghim.
63
- pH mi trng m o ca cc l chut hu nh khng b nh hng trong sut thi gian
th nghim.
Nh vy, th nghim tc dng ni tit kiu oestrogen ca cao chit t sn dy cho kt qu
dng tnh vi liu 150 mg / chut / ngy, cao chit c tc dng ni tit kiu oestrogen vi kt
qu l 51,5%.
So snh vi kt qu th nghim phng ng y thc nghim, Vin y hc c truyn Vit
Nam th liu th nghim ca cao chit isoflavone t sn dy tng i cao ( c ba l l 50 mg /
con / ngy, u tng l 25 mg / con / ngy). Tuy nhin, kt qu th nghim c biu hin
dng tnh trn 51,5% s chut cho thy cao chit isoflavone t sn dy c tc dng ni tit kiu
oestrogen kh r rt.
Sau y l hnh nh mt s tiu bn v s xut hin cc t bo sng cc l chut th nghim:
Hnh 1. Tiu bn t bo ca chut l chng
Hnh 2. Tiu bn t bo ca chut l chun Hnh 3. Tiu bn t bo ca chut l th
IV. KT LUN
Cao chit isoflavone t c sn dy Pueraria thomsonii Benth. c hot tnh ni tit kiu
estrogen trn 51,5% chut nht trng ci vi liu ung 150 mg / con / ngy. iu ny cho thy
hot tnh estrogen ca hn hp isoflavone chit xut t c sn dy l kh r rt.
64
Li cm n. Tc gi xin trn trong cm on Hip hi cc trung dai hoc Vuong quc Bi v hop
tc pht trin trung dai hoc (Flemish Interuniversity Council for University Development
cooperation - VLIR UOS) d ti tro kinh ph gip chng ti thuc hin nghin cu ny.
TI LIU THAM KHO
1. Bi ging dc liu - Tp 1, Trng i hc Dc H Ni.
2. Cy thuc v ng vt lm thuc Vit Nam - Tp 2, Nh xut bn Khoa hc v K thut,
H Ni, 2004.
3. V Vn Chi - T in cy thuc Vit Nam, Nh xut bn Y hc, 1999.
4. Dc in Vit Nam - B Y t, 2002.
5. Tt Li - Nhng cy thuc v v thuc Vit Nam, Nh xut bn Y hc, 2001.
6. P. Diel, S. Schumidt, and G. Vollmer - In vivo test system for quantitative and qualitative
analysis of the biological activity of phytoestrogens, Journal of chromatography B, 777
(2002) 192-201.
7. Joan Garey, Maria A. Morgan and et al - Effects of the Phytoestrogen coumestrol on locomotor
and fear- related behavior in female mice, Hormone and Behavior 40 (2001) 65- 76.
8. Lisa A. Henry and Diane M. Witt - Phytoestrogen effects on reproductive physiplogy and
behavior in female rats, Hormone and Behavior 41 (2001) 220-228.
9. Stefan O. Mueller - Overview of in vitro tools to assess the estrogenic and antiestrogenic
activity of phytoestrogens, Journal of chromatography B 777 (2002) 155-165.
SUMMARY
RESEARCH IN VIVO ON ESTROGEN ACTIVITY OF ISOFLAVONES FROM KUDZU
PUERARIA THOMSONII BENTH.
Kudzu is the popular medicinal herb in Vietnam. For a long time, Vietnamese have used
Kudzu roots powder to prepare the refreshing water in summer. In the traditional medicine,
kudzu root is used alone or is combined with the others to treat many of disease. Kudzu root has
not only refreshing and detoxifying effect, but it contains also isoflavones. They have a week
estrogen activity (named phytoestrogens), they can function as anti-estrogens by competing with
the more potent endogenous estrogens for binding to the estrogen receptor. Moreover,
phytoestrogens have high antioxidant effect and can promote bone health. Phytoestrogens can
prevent and treat osteoporosis, heart disease, breast cancer, prostate cancer and symptoms
associated with menopause of woman. Recent studies showed that phytoestrogens specifically
inhibit the activity of various growth-regulating enzymes, so that they have the cancer cell
growth inhibitory effects and they can also against non-hormone related cancers, such as cancer
of the colon, rectum, lung and stomach.
In this paper, we study on the estrogen activity of isoflavones from kudzu root Pueraria
thomsonii Benth planted in Hoabinh province of Vietnam. Research result showed that the
isoflavone extract from Pueraria thomsonii Benth. have estrogen activity on 51.5% of tested
white female mice with the oral dose 150 mg / mouse / day.
a ch: Nhn bi ngy 22 thng 2 nm 2005
Trng i hc Bch khoa H Ni.