Introduction of Pharma analysis

--Submitted by T. Arul solomon, M.Pharm Dept of Pharmaceutical analysis Asst. professor, Jagans college of pharamacy,Nellore

Introduction of pharma analysis

Introduction
Definition Types of analysis Applications

Definition

Pharmaceutical analysis involves Identification Determination Quantification Purification of a substance Separation of components of a solution or mixture Determination of structure of chemical compounds

Types
1.Qualitative 2.Quantitative analysis

1.Qualitative Identification
Detection of evolved gas Formation of precipitates Limit tests Colour change reactions Melting point Boiling point test etc.,

Types
1.Qualitative 2.Quantitative analysis

2. Quantitative Estimation
Chemical reations Instrumental reactions
Electrical measurements Spectroscopy methods
Absorption Emission

Chromatography

Bio assays and microbiological assays

Chemical reactions
Titrations
Acid base titrations Complexometric titrations Redox titrations Non aqueous titrations Precipitation titrations

Gravimetric methods Gasometric analysis

Electrical measurements
Potentiometry Conductometry Polarography Voltametry Amperometry

Spectroscopy methods
Absorption
UV-Visible Infra red Atomic absorption spectroscopy NMR

Emission spectroscopy
Flame emission spectroscopy Fluorimetry

 Chromatography methods
Gas chromatography HPLC TLC HPTLC Paper chromatography

Others
Mass Refractive index Specific rotation etc.,

Bio assays and microbiological assays

Potency of a substance is estimated using micro organisms, animal tissues or intact animals

Applications
In pharma industry
Quality control Quality assurance Analytical Research and development etc.,

In geographical surveys
To analyse soil samples

In pollution control
To analyse air samples, water, soil etc.,