The metabolic activity involved in microbiological methanation is dependent on the following factors: 1. Substrate temperature 2. pH level 3. Mixing Ratio 4. Loading Rate 5. Hydraulic Retention time 6. Nitrogen inhibition 7. C/N ratio 8. Agitation 9. Toxicity 10. Solid concentration 11. Seeding 12. Metal Cations 13. Particle size 14. Additives 15. BOD 16. COD 17. Heating Posted 4th March 2012 by Aiman Fatima