Lentiviral Vector Particle

Partner in gene function and drug target validation

Lentiviral Vector Particle

96 20-200 L 1-10 L 10 L 200 L

1.5Ep 500
mL

MOImultiplicity of infection
80%
MOI MOI
Polybrene 4-10 g/mLpolybrene
3-4
10-20
ENi.S.Enhanced Infection Solution(ENi.S.)
ENi.S. polybrene
ENi.S.

Lentivirus 293TBHK21 24
GFP RFP
GFPRFP
72-96 GFPRFP
GFPRFP Lentivirus

720 1 201203
8621 51320189 8621 51370635

Shanghai GeneChem Co.,Ltd

Building 1,720 CaiLun Road, Pu Dong,Shanghai,201203 P.R.C.
TE:(86)21 51320189 FAX:(86)21 51370635

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Lentiviral Vector Particle

1
1) 3X103~5X103 96
90 L 20

3050% Lentivirus

2) MOI

5 g/mL polybrene ENi.S.

ENi.S. 5 g/mL polybrene
3) 2 1.5 mL Ep 45 L ENi.S.
5 L 1X108 TU/mL (-80)
5 L
1X107 TU/mL 1X106
TU/mL 1X108 TU/mL ENi.S.
1X108 TU/mL
4) Ep 2 L 10 mg/ mL polybrene 400 L
polybrene polybrene 10 L 100 L
polybrene 2000 5 g/ mL
5) 10 L 1X106
TU1X105 TU1X104 TU 1 X104
MOI 100101
6) polybrene 10 L polybrene
7)

8) 8-12
9) 3-4

10)

720 1 201203
8621 51320189 8621 51370635

Shanghai GeneChem Co.,Ltd

Building 1,720 CaiLun Road, Pu Dong,Shanghai,201203 P.R.C.
TE:(86)21 51320189 FAX:(86)21 51370635

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Lentiviral Vector Particle

1-U251

MOI 1 U251

a) U251
b) MOI 1 80%
c) ENi.S. 5 g/mL polybrene
2.

MOI 100

MOI

720 1 201203
8621 51320189 8621 51370635

Shanghai GeneChem Co.,Ltd

Building 1,720 CaiLun Road, Pu Dong,Shanghai,201203 P.R.C.
TE:(86)21 51320189 FAX:(86)21 51370635

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Lentiviral Vector Particle

2
1)
5X103 96 90 l
10 5X104 900 L ENi.S. 90 L
5X103 / 10
2) 2
3) polybrene 34
4) polybrene 56
5)

6) 8-12 50 L 100 L
7) 3-4

8)
3. KM3

MOI 100 KM3

a) KM3
b) Eni.S.
c) 5 g/mL polybrene 2-3
d)

5 g/mL polybrene 80%

MOI 50

720 1 201203
8621 51320189 8621 51370635

Shanghai GeneChem Co.,Ltd

Building 1,720 CaiLun Road, Pu Dong,Shanghai,201203 P.R.C.
TE:(86)21 51320189 FAX:(86)21 51370635

Page 5 of 5