(?

)

( Hb )


= ,
= / ( 1-2 )
= 20

1. (Screening test)
OFT
DCIP
Hb E Screen
MCV
2. (Confirmation test)
HPLC

Hb A2 typing

A2 Microcolumn
PCR : - thal trait


OFT

DCIP E Screen
( Hb E-trait )

A2 typing
-HPLC
-A2 Microcolumn
B thal/HbE

PCR

- thal trait

% Hb A2 E
Result
Normal
-thal trait
HbE trait
-thal / HbE disease
Homozygous HbE

Hb A2
<4 %
4-9 %
10-30 %
30-60 %
>60 %


OF
Negative
-thal trait
-thal trait
Hb E trait

E screen

+
Negative

Positive

Hb E trait

Hb E-trait


OF

E- Screen

Positive

-thal trait
-thal trait
Hb E trait Homo. Hb E


OF
Positive

PCR
-thal trait

Hb typing
-thal trait
Hb E thal trait
Homo Hb E

 LAB
OFT

Negative

E screen
Negative

Normal

 LAB
OFT

Negative

E screen
Positive

Hb E-trait

 LAB
OFT

E screen

Positive

-trait

A2
4.8%

PCR
Negative

 LAB
OFT

Positive

E screen

A2
2.2%

-1 trait

PCR
Positive

 LAB
OFT

Positive

E screen

A2
5.1%

-trait /-1 trait

PCR
Positive

 LAB
OFT

Positive

E screen

A2
65.5%

HomozygouseHb E

PCR
Negative

 LAB
OFT

Positive

E screen

A2
45.5%

-trait/Hb E

PCR
Negative


Homozygous b-thalassemia

b-thal
b

-thalassemia/ HbE diseases

b-thal

Hb Barts hydrops fetalis

a-thal 1 +

b-thal
Hb E
a-thal 1


Hb

Hb E

Hb E

alpha-trait

beta-trait +

Normal

alpha-trait

Case study
test

OFT

Pos

Neg

Neg

E-screen
MCV

82

A2

3.1

PCR

Case study
test

OFT

Pos

Neg

E-screen

Pos

MCV

80

82

A2

23.0

25.2

PCR

Case study
test

OFT

Pos

Pos

MCV

65

68

A2

5.6

6.2

PCR

Neg

Neg

E-screen

Case study
test

OFT

Pos

Pos

MCV

68

65

A2

2.1

2.3

PCR

Pos

Pos

E-screen

Case study
test

OFT

Pos

Pos

MCV

80

72

A2

23.1

25.2

PCR

Neg

Pos

E-screen

Case study
test

OFT

Pos

Neg

MCV

62

Pos
82

A2

4.5

25.2

PCR

Pos

Neg

E-screen

Case study
test

OFT

Pos

Pos

MCV

69

70

A2

2.8

25.2

PCR

Pos

Neg

E-screen

Case study
test

OFT

Pos

Pos

MCV

79

71

A2

28.0

25.2

PCR

Neg

Pos

E-screen

Case study
test

OFT

Pos

Pos

56
2.0

65
2.1

PCR

Pos

Pos

Hb Cs

A2ACs

E-screen
MCV

A2

Case study
test

OFT

Pos

Pos

E-screen
MCV

60

65

A2

4.5

25.2

PCR

Pos

Pos

Case study
test

OFT

Pos

Pos

MCV

75

69

A2

26.4

56

PCR

Neg

Neg

E-screen

Case study
test

OFT

Pos

Pos

MCV

69

68

A2

20.0

6.5

PCR

Pos

Neg

E-screen

Case study
test

OFT

Neg

Neg

E-screen
MCV

Neg

Neg

A2
PCR

Case study
test

OFT

Neg

E-screen
MCV

Neg
-

A2

PCR

Case study
test

OFT

Pos

E-screen
MCV

78

A2

3.1

PCR

Neg