ZOOLOGY 102 Instruction for ELGA 1. 2. 3. 4. Ready your list of vertebrates for analysis.

It is very important that the spellings are correct. Likewise, be sure to download DNA Baser and MEGA 5.2.2 (Windows) or chose the one for Mac. Copy this url http://www.ncbi.nlm.nih.gov/genbank/. This opens to the website of the Genbank. Paste your query species on the blank. Add the name of the gene sequence we are looking for. Then click search. For example: Rhacophorus everetti 16S ribosomal RNA gene; click search We shall use 16S ribosomal RNA gene as our target gene of sequence for the analysis. 5. 6. 7. It will show you a list of results that fits your search. Click one that perfectly matches your search. Scroll down below until you see FEATURES at the left column. Under this is the gene (in blue text) of target. Click this. After clicking, you will see at the lower right corner the text below. Display: FASTA GenBank Help 8. 9. Click FASTA. This is the file format used to save the file. Upon clicking, it will show you

10. Click send to. Choose file, then click create file to download the sequence in FASTA format.

11. After downloading, click the file. Choose DNA Baser to open the file. This box with the sequence will appear.

12. Click save. Change the file name with this format for uniformity. R. pardalis 16S rRNA 13. Be sure to have a folder for all the sequences for easy access. 14. End.

Species Coptotermes formosanus

Bases >gi|32264065|gb|AY302708.1| Coptotermes formosanus 16S ribosomal RNA gene, partial sequence; mitochondrial gene for mitochondrial product CTTAATCCAACATCGAGGTCGCAAACCTCCCTGCCGATAAGAACTCTCAAGGAAGATAACGCTGTTATCC CTAAGGTAATTTAATCTTATAATCAAAATAAATGGATCAAATAAATATAAATCAATATAACCAAAATCAA GAGGAGTTAATTATATTCCTCCCATCACCCCAACAAAACAAAAAGTCCACTCACCAATAAAACAAACAAA CAACAAACAAACAAATAAACCAAATGTTAAACTCTATAGGGTCTTCTCGTCCCACAAAAACATCTAAGAA TTTTAACTCAAAAACCAAATTCAATAAAACATTCAACATTAAGACAGCTTGTGCCTCGTCAAGCCATTCA TTCCAGATCACAATTAAAGAACTAATGATTATGCTACCTTTGCACGGTCAAAATACCGCGGCCCTTCAAC ACTCAAGTCAGCGGGCAGGCCATACTTCAAAAACTAA

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