Regulation of T-Cell Migration to the Skin by Shiitake: Relevance to Psoriasis Targeted Delivery of Mushroom Extracts for Treatment of Psoriasis

Jack Hay Moon Bay Biologics Amer Kalaaji Mayo Dermatology Alan Young SDIP/TranzDerm Solutions

Skin anatomy& Physiology

Largest organ
Area: 1.8m2 1-2mm thick Weighs 16% of TBW

Three layers
Epidermis Dermis Hypodermis

What is Psoriasis?
Is a chronic multifactorial inflammatory skin disease
Greater proliferative activity Rapid migration of keratinocytes Shortened cell cycle

Facts& Figures of Psoriasis

125 million people worldwide (2 to 3 percent of the total population) 7.5 million Americans (150, 000 to 260,000 new cases each year) Severity of the disease
mild (<3% of body), moderate (3-10% of body) severe (>10% of body)

Approaches for Topical delivery

A recognized treatment for Psoriasis involves regional delivery of Vitamin D, which appears to ameliorate symptoms Vitamin D also has been demonstrated to downregulate the immune response, which could limit disease if modulated appropriately.
Mushrooms contain significant amounts of Vitamin D and its precursor ergosterol

Vitamin D Content of Mushrooms

Variety Vitamin D Content Ergosterol (precursor of Vitamin D2) White Button Crimini Portabella Shiitake Oyster Morel Enoki

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*From Phillips et al., 2011 J. Agric. Food Chem 59(14)7841-7853

Core-Shell Zein Nanoparticles

In vitro-human psoriatic skin Penetration

12
10 Drug (g/cm2) Receptor Skin Psoriatic Skin

8
6 4 2 0
Solution Nanoparticles Solution Nanoparticles

Uninvolved Skin

Figure: In-vitro skin penetration of test solution and nanoparticles through uninvolved human skin and psoriatic human skin. The formulation was treated for 48 hrs. Values are mean SD (n=4). Significant difference at p< 0.05.

The Project
Evaluate the use of Zein-based nanoparticles for delivery of natural mushroom extracts in relief of psoriatic symptoms

Green Tech
Potentially immunologically active to limit inflammation Potential for alternative therapy for psoriasis.