B CNG THNG

TRNG I HC CNG NGHIP THC PHM KHOA CNG NGH THC PHM

Phng php xc nh nhit h ha v xc nh hm lng tinh bt ca nguyn liu

Nhm: 5 Lp : 04CDNKN2 Mn : Kim tra cht lng lng thc GVHD : Vnh Long

1. Phng php xc nh nhit h ha:

Xc nh nhit h ho qua phn hu kim

Nhit h ha cui cng ca tinh bt go c phn chia: - Thp: <70oC - Trung bnh: 70-74oC - Cao: >74oC

 phn hy kim ca ht go xt uc nh gi qua thng im t 1-7 v t l nghch vi nhit h ha:

phn hy kim 1-2-3 4-5 6-7 Nhit h ha Cao Trung bnh Thp

1.2 Ni dung phng php: Nguyn tc: Dng dung dch KOH 1,7% phn hu 6 ht go xt nguyn nhit 30oCtrong 23 gi. Da vo hnh dng v mc b phn hu ca cc ht go sau khi m xc nh phn hu kim bng cch so snh mu go th nghim vi mu chun v thang im chun, t qui ra nhit h ho ca mu.

Dng c v ha cht: - Hp petri - T m c kh nng duy tr nhit 30oC - Pipep 10ml - Dung dch KOH 1,7%
Cch tin hnh: - T mu go xay xt lm sch cm chn ly khong 30 ht go nguyn.

Ly 6 ht go xt nguyn t vo hp petri sp xp sao cho cc ht go khng chm vo nhau. Dng pipep cho vo hp khong 10ml dung dch KOH 1,7% ( dung dch KOH ngp go). y hp petri li v to = 30oC trong khong 23 gi. Sau mt thi gian, ly hp petri ra v quan st hnh dng, mc b kim phn hy ca tng ht trong mu ri da vo thang im nh gi.

nh gi thang im: - im 1: Ht go khng b phn hy. - im 2: Ht go b trng ln. - im 3: Ht go b trng ln, vnh keo khng hon thin v hp. - im 4: Ht go b trng ln vnh keo hon chnh v rng. - im 5: Ht go b nt ra hoc v thnh nhng mu nh, vnh keo hon chnh v rng. - im 6: Ht go b phn tn, ha tan vi vnh keo. - im 7: Ht go b phn tn v trn ln hon ton.

2. Xc nh hm lng tinh bt:

Xc nh hm lng tinh bt theo phng php thu phn bng acid Nguyn tc: Tinh bt s b thu phn to thnh ng Glucoza di tc dng ca acid. Xc nh hm lng Glucoza to thnh ri nhn vi h s 0.9 ta c hm lng tinh bt.

Dng c v ha cht:
- Cn phn tch
Phu lc v giy lc Phu lc G-4 Buret 25ml Pippet bu 20ml

- Bnh nh mc 250ml
Bnh tam gic 100 v 250ml My bunzen ng ong 50ml Dd Feling A v B

Dd Fe2(SO4)3 Dd Na2SO4 bo ho Dung dch NaOH 20% Dung dch Iod.

- Dd ch axetat 10% - DdHCl m c - Dung dch KMnO4 0,1 N

Cch tin hnh:

- Cn 2g mu th nghim, nghin nh, cho vo bnh tam gic 250ml - Thm 50ml nc ct m, lc u, yn 30 pht - Lc, thu ra lng tinh bt bng nc ct 2-3 ln. - Chuyn ton b vo bnh tam gic 100ml, ht 6ml HCl m c ri cho vo bnh

y kn bnh bng nt cao su c lp ng sinh hn hi lu, un cch thu 3-5 gi. Lm ngui, trung ho hn hp bng dung dch NaOH 20%. Chuyn vo bnh nh mc 250ml, cho thm 10ml ch axetat 10%, lc u v cho thm 510ml Na2SO4 bo ho, nh mc v lc. Ht 20ml dung dch va nh mc vo bnh tam gic 250ml, thm 20ml dung dch Feling A, 20ml dung dch Feling B.

un si hn hp 3 pht, lng, lc bng phu lc xp chuyn dng G-4 vo bnh lc chn khng Bunzen. Ho tan kt ta bng dung dch Fe2(SO4)3 Chuyn ton b dung dch thu c vo bnh tam gic em i chun bng dung dch KMnO4 0,1 N n khi xut hin mu hng nht bn trong 30 giy. Ghi li th tch KMnO4 tiu tn.

Tnh kt qu: Hm lng tinh bt c tnh bng % theo cng thc:

Trong : m1: s mg Glucoza tra bng V : dung tch bnh nh mc (ml) V1: th tch sau khi lc (ml) m : khi lng mu th nghim (g)