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Seeds of the A. squamosa Linn. premature fruits were collected, shade and air dried and ground in to powder.

150 to 200 g of the powder was packed in filter paper packs and extracted with ethanol in a soxhlet apparatus continuously for about 6 to 10 h. Then the ethanolic extract was distilled off till semisolid substances were obtained. After evaporated to dryness, these substances were stored in a 20C deep freezer until further analysis.

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