Results

Ladders
1 kb 100 bp

Lane 3

Fig. 1. PCR Electrophoresis Gel The inserted unknown Mtb gene of the prey plasmid was amplified using PCR and the sequenced. A 1 kb and 100 bp ladder were used. Lane 3 and Lane 6 were positive for PCR product. Lane 3 was chosen for sequencing. The size of the prey insert was 2.5 kb.
0.6

Mean

Absorbance Mean

0.5 0.4 0.3 0.2 0.1 0

Positive control

Negative control

Isolate

Fig. 1. Liquid X-Gal Assay .5 OD of yeast culture was added to the isolate and centrifuged for 5 min. at 2000 x g. The negative control carried Rv3868 bait with an empty prey plasmid. The positive control carried Rv3868 bait and an EspC prey, a protein known to interact with Rv3868 ATPase. Using the HTX Beta-Galactosidase Assay Kit (DualSystems Biotech, Inc.), 100 ml of x-gal assay was added to the cell pellet and incubated at 30 C for 1 hour. The X-gal assay determined the strength of interaction between the bait and prey protein. The absorbance mean for our isolate was quite strong, indicating strong interaction between the bait and prey protein.