Microscopes

By VENKATA RAMANAN D S

MICROSCOPY
SCIENCE WHICH DEALS WITH THE STUDY OF MICRO/ SMALL THINGS, NOT VISIBLE TO THE NAKED EYE.

History of the Microscope

1590 first compound microscope

History of the Microscope 1655 Robert Hooke used a compound microscope to observe pores in cork He called them cells

History of the Microscope Antoine van Leeuwenhoek st 1 to see single-celled organisms in pond water

Microscope Vocabulary
Magnification: increase of an objects apparent size Resolution: power to show details clearly Both are needed to see a clear image

Ocular Body tube Revolving nosepiece Objectives

Scanning objective Low power objective High power objective Oil immersion objective

Eyepiece - 10X light tight tube change objectives second set of lens
4X 10X 40 or 43X 97 or 100X

Types of Microscopes

Compound Light Microscope 1st type of microscope, most widely used light passes through 2 lenses Can magnify up to 2000x

Ocular lens

Objective lenses

Types of Microscopes
Electron Microscope Used to observe VERY small objects: viruses, DNA, parts of cells Uses beams of electrons rather than light Much more powerful

Types of Microscopes

Transmission Electron Microscope (TEM) Can magnify up to 250,000x

FLUORESCENT MICROSCOPE

Types of Microscopes

Scanning Electron Microscope (SEM) Can magnify up to 100,000x

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Electron Beam
Specimen inclination

Backscattered Detector

Incident Beam
X-rays
Through Thickness Composition Information

Backscattered Electrons
Atomic Number (Z)

Cathodoluminescence
Electrical Information

Secondary Electrons
Topographic Information

Sample
Specimen Current
Electrical Information

THANK YOU

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