- Isolation of Caffeine from Tea & Melting Point Determination

Refluxingextractiondistillation melting point

determination

1. (g): 10
2. Caffeine (g): 0.03
3. 10 caffeine (%): 0.3
4. () caffeine (%)
PS.

(%)

1. ()

2. ()

17 21

25 29

3. ()

10 14

4. ()

11 15

5. ()
6. ()

18 22
4

13

0.3 / 0.8/2.4
1.1/1

26 30
12 16

7. ()

19 23

27 31

8. ()

20 24

28 32

5.

1 / 0.6

0.6

Pure Caffeine :

214~222

Caffeine () : 210~226

1. ?

(mg/mL)
()
1
1

(1)

(2)
i.

A.
(a)
I.
II.
III. stress by pests


IV. shade-grown teas
(b)
B. (plant varietal)
C. Age
(a)
(b) ()
I.
II.

ii.

A.
B.
C.

iii.

A.
B.
C.

2.(w/w,%)

1
2
3
4
5
6
7
8

3.

4. Refluxingextractiondistillation and melting point

determination

Refluxing
Filtration

1. refluxing

2. (1)
(2)

1.

2.

30C

1. extraction CH2Cl2
39C

Extraction 1. 1.

2. 1.

2.

2.
1.

2.

 Emulsion

()

1.
2. 5ml

sample

Simple

distillation

100ml


sample

1.
(1)

2.
(1)
(a)

(b)

(2) ( 1~2C )
(3)

3.
(1)