PAPER 3

SECTION A
CHAPTER 4
QUESTION
1.

A group of students carried out an experiment to show the effects of temperature on

the rate of an enzyme reaction. They used amylase as the enzyme and starch as the
substrate.
Sekumpulan murid telah menjalankan satu eksperimen untuk menunjukkan kesan
suhu ke atas kadar tindak balas enzim. Mereka menggunakan amilase sebagai
enzim dan kanji sebagai substrat.

Diagram 1/Rajah1
A mixture of amylase and starch suspension is immersed in a water bath set at
temperature 5C as shown in Diagram above (a). At one-minute interval, a drop of
the mixture is transferred onto a tile containing iodine as shown in
Diagram above (b). The time taken when the mixture failed to turn blue-black when
tested with iodine is recorded. The step is repeated for the mixtures of amylase and
starch at different temperatures. Table A shows the results of the experiment.
Satu campuran amilase dan ampaian kanji direndam di dalam kukus air pada suhu
5C seperti yang ditunjukkan dalam Rajah di atas(a). Pada selang satu minit,
setitik campuran dipindahkan ke atas jubin yang mengandungi iodin seperti yang
ditunjukkan dalam Rajah di atas(b). Masa diambil apabila campuran gagal untuk
bertukar biru-hitam apabila di uji dengan iodin direkodkan. Langkah diulangi bagi
campuran amilase dan kanji pada suhu yang berbeza. Jadual A menunjukkan hasil
eksperimen tersebut.

Table A/Jadual A
(a) State two observations made from this experiment.
Nyatakan dua pemerhatian daripada eksperimen ini.
1.
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2. ______________________________________________________________
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[3 marks/markah]

(b) State two inferences that can be made from your observations in (a).
Nyatakan dua inferens yang boleh dibuat daripada pemerhatian di (a).
1.

______________________________________________________________
_____________________________________________________________
______________________________________________________________

2.
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[3 marks/markah
]
(c) Record the time taken in the boxes provided in Table A for each temperature,
when the mixtures fail to turn blue-black when tested with iodine solution.
Rekod masa yang diambil dalam ruang yang disediakan dalam Jadual A bagi
setiap suhu, apabila campuran gagal bertukar menjadi biru-hitam apabila diuji
dengan larutan iodin.
[3 marks/markah
]
(d) State the variables in Table B.
Nyatakan pembolehubah dalam Jadual B.
Variables
Pembolehubah

Method to handle the variable

Cara mengendali pembolehubah

Manipulated variable
Pembolehubah dimanipulasikan

__________________________

__________________________

__________________________

__________________________

__________________________

Responding variable
Pembolehubah bergerak balas

__________________________

__________________________

__________________________

__________________________

__________________________

Constant variable
Pembolehubah malar

__________________________

__________________________

__________________________

__________________________

__________________________

Table B/Jadual B
[3 marks/markah]

(e) State one hypothesis for this experiment.

Nyatakan satu hipotesis bagi eksperimen ini.
________________________________________________________________
________________________________________________________________
[3 marks/markah]
(f) Based on Table 1 construct a table and record the results of the experiment
which includes the following aspects:

(g) i.

ii.

Temperature of water bath

Time taken when the mixture failed to turn blue-black when
tested with iodine
Rate of enzyme reaction
[3 marks/markah]

Based on your results in 1 (f), plot a graph showing the rate of enzyme
reaction against temperature.
Berdasarkan keputusan dalam Jadual A, plot graf yang menunjukkan
kadar tindak balas enzim melawan suhu.
[3 marks/markah]
Based on the graph you have plotted, describe the relationship between the
rate of reaction, which is catalysed by amylase and the temperature.
Berdasarkan graf yang telah anda plot, terangkan hubungan antara kadar
tindak balas, di mana pemangkin adalah amilase dan suhu.
____________________________________________________________
____________________________________________________________
____________________________________________________________
____________________________________________________________

[3 marks/markah]
(h) What can be deduced about the activity of amylase on starch based on the
results of the experiment?
Apakah yang boleh disimpulkan mengenai aktiviti amilase pada kanji
berdasarkan keputusan eksperimen.
_______________________________________________________________
_______________________________________________________________
_______________________________________________________________
[3 marks/markah]

(i) The experiment is repeated by fixing the medium temperature at 37C but
changing the pH of the medium at 3. Predict the change of the colour of the
iodine solution in this experiment. Explain your answer.
Eksperimen diulang dengan menetapkan suhu medium pada 37C tetapi
mengubah pH medium pada 3. Ramalkan perubahan warna larutan iodin
dalam eksperimen ini. Terangkan jawapan anda.
_______________________________________________________________
_______________________________________________________________
_______________________________________________________________
[3 marks/markah]
(j) In Table C, list all the materials and apparatus used in the experiment as in the
Diagram 1.
Dalam jadual C, senaraikan semua bahan dan radas yang digunakan dalam
eksperiment in seperti dalam Rajah 1.
Materials
Bahan

Apparatus
Radas

[3 marks/markah]