Scribd Logo
Upload

Welcome to Scribd!

  • Mechanisms of Antigen Processing For Class I MHC Presentation

    Uploaded by

    codearchitect
    12 views8 pages
    The document summarizes the mechanisms of antigen processing for class I MHC presentation. Antigen processing involves breaking down proteins into peptides, which are then transported into the ER by the TAP transporter and loaded onto MHC class I molecules to be presented to CD8 T cells. The proteasome degrades cytosolic proteins into peptides, which enter the ER through the TAP transporter and bind to partially folded MHC class I molecules assisted by chaperone proteins to generate peptide-MHC complexes.

    Original Description:

    Original Title

    4Q03 Presentation3

    Copyright

    © Attribution Non-Commercial (BY-NC)

    Available Formats

    PPTX, PDF, TXT or read online from Scribd

    Did you find this document useful?

    Is this content inappropriate?

    Report this Document
    The document summarizes the mechanisms of antigen processing for class I MHC presentation. Antigen processing involves breaking down proteins into peptides, which are then transported into the ER by the TAP transporter and loaded onto MHC class I molecules to be presented to CD8 T cells. The proteasome degrades cytosolic proteins into peptides, which enter the ER through the TAP transporter and bind to partially folded MHC class I molecules assisted by chaperone proteins to generate peptide-MHC complexes.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as PPTX, PDF, TXT or read online from Scribd
    Flag for inappropriate content
    12 views8 pages

    Mechanisms of Antigen Processing For Class I MHC Presentation

    Uploaded by

    codearchitect
    The document summarizes the mechanisms of antigen processing for class I MHC presentation. Antigen processing involves breaking down proteins into peptides, which are then transported into the ER by the TAP transporter and loaded onto MHC class I molecules to be presented to CD8 T cells. The proteasome degrades cytosolic proteins into peptides, which enter the ER through the TAP transporter and bind to partially folded MHC class I molecules assisted by chaperone proteins to generate peptide-MHC complexes.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as PPTX, PDF, TXT or read online from Scribd
    Flag for inappropriate content
    of 8

    MECHANISMS OF ANTIGEN PROCESSING FOR CLASS I MHC PRESENTATION

    By: Shelby Olesovsky and Muhammad Bin Aftab Syed

    Antigen Processing

    Antigen Processing:
    Create

    peptides from native proteins, prepares antigens for presentation to cells of immune system (T lymphoctyes) Stage of antigen presentation pathways

    Antigen Presentation:
    Display

    of peptide by MHC molecule at cell surface

    MHC I Molecule CD8 T-cells MHC II Mlecule CD4 T-cells


    From Janeways Immunobiology(Murphy, 2012)

    Basic Overview

    From Janeways Immunobiology(Murphy,

    Generating the Peptide

    Cytosolic protein degradation by proteasome (catalytic core and regulatory caps) Hollow core with active proteolytic subunits Proteins tagged for degradation with ubiquitin protein targets cap recognize and unfold protein enter core broken down to short peptides released into cytosol

    http://users.rcn.com/jkimball.ma.ultranet/BiologyPages/P/Proteaso me.html

    Peptide Entry into ER

    Peptides are continuously being transported from the cytosol to the ER ABC proteins: TAP1 and TAP2 form a heterodimer in ER membrane ATP-dependent peptide transporter ATP hydrolysis needed to internalize peptides into the ER Channel through which a peptide can enter ER lumen TAP1, TAP2 hydrophobic
    domains (transmembrane / ER lumen) and ATP-binding domain (cytosol) dimerize four domain transporter

    From Janeways Immunobiology(Murphy,

    Generating MHC Class I

    During synthesis MHC molecules are translocated to the ER lumen In the ER MHC molecules are in a partially folded state: MHC class 1chain must associate with 2microglobulin and then with a peptide chain binds to chaperone protein calnexin When2-microglobulin binds to chain, heterodimer dissociates from calnexin and binds to PLC

    Putting it All Together

    From Janeways Immunobiology(Murphy,

    References

    Murphy, K.M. (2012). Janeways Immunobiology (8th ed. pp. 8-9, & 13542). New York, NY: Garland Science Taylor & Francis Group. Creswell, P., Ackerman, A.L., Giodini, A., Peaper, D.R., & Wearsch, P.A. (2005). Mechanisms of MHC class I-restricted antigen processing and cross presentation. Immunol. Rev., 207, 145-57. Yewdell, J.W., & Bennink, J.R. (1999). Mechanisms of viral interference with MHC class I antigen processing and presentation. Aunnu. Rev. Cell Dev. Biol., 15, 579-606.

    You might also like