NHN DIN V PHN TCH T BO

Bi ging gm: I. S khc bit gia cc t bo II. To huyn ph t bo III. Cc phng php phn tch t bo

S KHC BIT GIA CC T BO I.1. Nguyn l phn tch t bo

phn tch mt loi t bo ra khi mt nhm nhiu t bo cn phi da vo c im khc bit ca t bo so vi cc t bo cn li trong nhm.
T s khc bit s a ra phng php phn tch nhm li dng s khc bit .

I.2. CC C IM KHC NHAU GIA CC LOI T BO

Gia cc loi t bo c th khc nhau v: 1. T trng t bo 2. Kch thc t bo Lng ng t trng Vn tc lng

3. Khng nguyn b mt t bo 4. Nhng thnh phn protein bn trong t bo

5. in tch b mt t bo
6. i lc b mt t bo cho pha ha tan

II. TO HUYN PH T BO
Hu ht trong phn tch t bo, t bo phi c huyn ph thnh t bo n:

Da vo c im khc bit gia cc t bo, cc nh khoa hc a ra cc phng php sau: - M lng: khng cn huyn ph

- M rn: tch m thnh t bo n

CC PHNG PHP PHN TCH T BO

V mt s phng php khc

III.1. VN TC LNG Phng php ny da vo lng ca t bo ph thuc vo t trng. Phng php tin hnh:

III.2. PHNG PHP LNG NG T TRNG

LI TM RA (CENTRIFUGAL ELUTRIATION)

Quy trnh tin hnh: (1)setting up and sterilizing the apparatus, (2)calibration, (3)loading the sample and establishing the equilibrium conditions, and (4) harvesting fractions.

TCH DA VO GN KT MIN DCH (IMMUNE PANNING)

K THUT TCH DA VO T TNH - MACS

K THUT FACS (PHN TCH T BO C KCH HIT HYNY QUANG)

Charged droplet sorter

Charged droplet sorter

Poor Recovery and Purity: Calibrating the drop delay

Measurement
Measure this distance L

Break off point How many drops in an equivalent length?

As the frequency is known the time taken for a cell to traverse L can be calculated. This time determines when to charge the stream as the required cell breaks off in a drop.

Espectros de Excitao / Emisso

350
300 nm 400 nm

457

488

514

610 632
600 nm 700 nm

500 nm

Texas Red

PI
Ethidium PE

FITC

H THNG PHN TCH T BO PALM v CT BNG TIA LAZER