ANTI-BACTERIAL ACTIVITY OF NEEM

INTRODUCTION

ANTIBACTERIAL PROPERTY OF NEEM:-

Oil from the leaves, seed and bark possesses a wide spectrum of antibacterial action against Gram-negative and Gram-positive microorganisms, including M. tuberculosis and streptomycin resistant strains. In vitro, it inhibits Vibrio cholerae Klebsiella pneumoniae, M. tuberculosis and M. pyogenes. Antimicrobial effects of neem extract have been demonstrated against Streptococcus mutans and S. faecalis.

EXPERIMENT TO DETERMINE THE ANTIBACTERIAL PROPERTY OF NEEM

AIM:- To determine the antibacterial property of

neem.

MATERIALS REQUIRED: GLASSWARES:- etriplates, test tubes, pipette, conical

flask, disposable sterile petriplates.

MATERIALS:- !otton swab, china dish, inoculation

loop, spatula etc".

BACTERIAL CULTURES:Staphy !"!""#$ a#%&#$ S' &p()&%*(t($ Sa *!+& a typh( E$"h&%("h(a "! ( , &-$(& a $p&"(&$ Ba"( #$ $#-t( ($

INSTRUMENTS:A#t!" a.& / T! $t&%( (0& -a"t&%(a H!t a(% !.&+ / T! $t&%( (0& 1 a$$ 2a%&$ I+"#-at!% / T! "# t#%& -a"t&%(a

METHOD: DAY ONE:-

The bacterial cultures are inoculated aseptically into sterile peptone water test tube #$ml% and incubated.
DAY TWO:TWO

After &' hours of incubation, the bacterial culture in the peptone water is inoculated on to the surface of sterile nutrient a1a% plate aseptically using sterile cotton swab. 3LAWN CULTURE4 After a few minutes, neem extract impregnated. (hatman filter paper disc is placed on cultured nutrient Agar plate and incubated on a incubator.

 DAY THREE:After &' hours of incubation the plates were observed for )one of inhibition.

S'NO:

BACTERIA STUDIED
Ba"( #$ $#-t( ($ E'"! ( , &-$(& a typh( Sa *!+& a typh( Staphy !"!""#$ a#%&#$ S'Ep()&%*(t($

DIAMETER OF 5ONE OF INHIBITION

66 ** 8 ** 67 ** ; ** = ** 6; **

6' 7' 9' :' <' 8'

*taphylococcus aureus Klebsiella species Bacillus species Stap. epidermitis E. coli Salmonella species