Multiple choice questions worth one mark each Circle the single most likely response Question 1 A microorganism

will grow inside and outside an anaerobic cabinet with optimum g rowth at pH 7.2 and a pH growth range of 5.2 to 8.2. The microorganism is: a) a strict anaerobe. b) a strict aerobe. c) an acidophile. d) a neutrophile. e) an alkalinophile. Question 2 E. coli grows well aerobically in a defined synthetic medium with glucose as the limiting and sole carbon/energy source. Under ANAEROBIC conditions: a) any colonies produced will be smaller than those grown in the presence of oxygen. b) there will be no bacterial growth. c) oxidation of glucose will be complete. d) the biomass of the stationary phase will be greater. Question 3 A microorganism is isolated from the mud of a lake bottom. It will grow in an an aerobic jar in only nutrient broth. The microorganism is: a) a photolithotroph. b) a photoorganotroph. c) a chemolithotroph. d) a chemoorganotroph. e) an autotroph.

Question 4 A microorganism will grow in the temperature range -2 to 22 C under a normal atm osphere -the microorganism is: a) a pyschrophile. b) a mesophile. c) a thermophile. d) a strict anaerobe. Question 5 Genera of endospore-forming bacteria include: a) Clostridium. b) Lactobacillus. c) Streptomyces. d) Myxococcus. Question 6 Which of the following is incorrect regarding bacterial pili or fimbriae: a) are exterior protein filaments. b) are generally involved in motility. c) can be involved in adhesion of the cells to surfaces. d) can be associated with the transfer of DNA by conjugation in some bacteria in the case of certain specialised pili. Question 7 Which of the following is incorrect regarding the bacterial cytoplasmic membrane : a) is a rigid structure at ambient temperature. b) contains phospholipids which are organised in a bilayer. c) contains specific protein permeases. d) may contain respiratory enzymes and ATPase

Question 8 Which of the following is incorrect: The outer membrane of Gram-negative bacteria is composed of: a) phospholipids. b) proteins. c) lipopolysaccharides. d) peptidoglycan.