Stains (Micro) Giemsa

PAS Ziehl-Neelsen (carbol fuchsin) India ink Silver stain

Organism(s) 1.Chlamydia 2.Borrelia 3.Rickettsiae 4.Trypanosomes 5.Plasmodium 1.Stains glycogen & mucopolysaccharides 2.Used to dx Whipples disease (Tropheryma whipplei) 1.Acid-fast bugs (Nocardia, Mycobacterium) 1.Cryptococcus neoformans (mucicarmine stains thick polysaccharide capsule red) 1.Fungi (Pneumocystis), Legionella, H. pylori

Stains (IHC) Vimentin Desmin Cytokeratin GFAP Neurofilaments

Cell Type CT Mm Epithelial cells Neuroglia Neurons

Organism H. flue Neisseria B. pertussis C. diphtheriae M. tuberculosis M. pneumoniae Lactose-fermenting enterics Legionella Fungi

Culture Requirements Chocolate agar w/factor V (NAD+) & X (hematin) Thayer-Martin (VPN) media vancomycin (inhibits gram bugs), polymyxin (inhibits gram except Neisseria; SE is neurotoxicity, i.e. numbness of extremities), nystatin (inhibits fungi) Bordet-Gengou agar Tellurite plate, Lofflers media Lowenstein-Jensen agar Eatons agar Pink colonies on MacConkeys agar (fermentation acid pink); E. coli is also grown on eosin-methylene blue (EMB) agar green metallic sheen CYE (charcoal yeast extract) agar buffered w/Cys & Fe Sabourauds agar