A

394 nm

0.2185

374nm

1.237

EM

c
1

1.00E-06

218500

=394
Eobs
[ ](M)
A
b (cm)
Y
X
1.00E-04
0.8964
0.1 8.9640E+04 2.78574E-05 3.59E+00
5.00E-05
2.03
0.4 1.0150E+05 2.06725E-05 2.418677
2.00E-05
2.078
1 1.0390E+05 1.32106E-05 1.513935
1.00E-05
0.9694
1 9.6940E+04 9.06995E-06 1.102543
5.00E-06
0.5459
1 1.0918E+05 6.76293E-06 0.739324
1.00E-06
0.2185
1 2.1850E+05
#DIV/0!
0
=374
Eobs
[ ](M)
A
b (cm)
Y
X
1.00E-04
1.237
0.1 1.2370E+05 3.24785E-05 3.078961
5.00E-05
2.482
0.4 1.2410E+05 2.30144E-05 2.172556
2.00E-05
2.265
1 1.1325E+05 1.37849E-05 1.450862
1.00E-05
0.9036
1 9.0360E+04
8.834E-06 1.131989
5.00E-06
0.4533
1 9.0660E+04 6.25391E-06
0.7995
1.00E-06
0.1445
1 1.4450E+05 3.67607E-06 0.272029

ED374
237000

G394nm
G374nm

ED394

KD374

187000 5.00E+06

-3.82E+04 J/mol
-3.77E+04 J/mol

KD394
4.00E+06

sqrt [T]/(Em-Eobs) vs. sqrt [T](Em-Eobs)

for monomer
0.00003

y = 8E-06x + 1E-06

0.000025
394

0.00002

Linear (394)
0.000015
0.00001
0.000005
0
0

0.5

1.5

2.5

3.5

sqrt [T]/(Em-Eobs) vs. sqrt [T](Em-Eobs)

for dimer

y = 1E-05x - 1E-06

0.000035
0.00003
0.000025
0.00002

374 nm

0.000015

Linear (374 nm)

0.00001
0.000005
0
0

0.5

1.5

2.5

3.5