ARTIKEL ILMIAH

PEMBERIAN FRAKSI AIR KULIT BUAH JERUK KEPROK (Citrus nobilis Lour.) TERHADAP KADAR NITROGEN UREA DARAH DALAM SERUM DARAH MENCIT (Mus musculus) JANTAN

Oleh : DE I RO ITA !"!"#!$%&

FAKULTAS KEDOKTERAN HE'AN UNI ERSITAS AIRLANGGA SURABA(A $!#!

THE EFFECT OF 'ATER FRACTION OF CITRUS NOBILIS LOUR. PEELS TO'ARD BLOOD UREA NITROGEN LE ELS IN THE BLOOD SERUM OF

MALE MICE (Mus Musculus)

1)

')*+),-). $) De/) Ro/)-,. 0) B,12,34 Poer3o1o. 5) Ir, S,r) (u*,3),6,3-). Departemen Anatomi Veteriner, 2)Mahasiswa, 3) Departemen Anatomi Veteriner, 4)Departemen Klini Veteriner !a "ltas Ke#o teran Hewan $ni%ersitas Airlan&&a A27-r,8De/) Ro/)-, ABSTRACT

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A lot o' "se'"l (ontents 'rom the peels o' Citrus nobilis Lo"r) s"(h as al aloi#, 'la%onoi#, pol*phenols an# hesperi#in whi(h (an '"n(tion as an anti'ertilit*) In a##ition, it (ontains harm'"l (ontent s"(h as saponin whi(h ma* #ama&e the i#ne*s an# a''e(t the e+(retion o' "rea in the ,loo#) -"rpose o' this resear(h is to #ete(t in'l"en(e #istri,"tion o' water 'ra(tion o' Citrus nobilis Lo"r) peels to ,loo# "rea nitro&en le%els in ,loo# ser"m o' male mi(e . Mus musculus)) This e+periment "se# 24 male mi(e, ran#oml* #i%i#e# into 'o"r #i''erent treatment an# si+ repli(ates ea(h) Ea(h treatment .-1/ 40 m&1 & ,o#* wei&ht, -2/ 20 m&1 & ,o#* wei&ht, -3/ 30 m&1 & ,o#* wei&ht respe(ti%el*) a#ministere# water 'ra(tion Citrus nobilis Lo"r) 1 (( orall* 'or 34 #a*s, e+(ept in the (ontrol &ro"p .-0) who were treate# with 1 (( o' #istille# water 'or 34 #a*s) This resear(h was anal*5e# "sin& 6ompletel* Ran#omi5e# Desi&n an# (ontin"e# with Least 7i&ni'i(ant Di''eren(e Test with 48 si&ni'i(an(e le%el to now the #i''erent treatments) The Res"lts showe# there are si&ni'i(ant #i''eren(es o' ,loo# "rea nitro&en le%els in the 'o"r treatment &ro"ps .p 90)04), in (ases there was in(rease# in -1 treatment &ro"p ,"t eep in the normal limit) There is no si&ni'i(antl* #i''eren(es on the remaine# &ro"ps .-2 : -3) Key Words/ water 'ra(tion o' Citrus nobilis Lo"r) peels, ;loo# $rea <itro&en .;$<) le%els, anti'ertilit* Men*et"="i "nt" #ip",li asi an 7"ra,a*a, 12 >"li 2010 Mahasiswa .De%i Ro%ita) <IM) 0202102?3 Men*et"="i Dosen Ter ait I .Retno ;i=anti, M7), #rh) <I-) 1A440223A1032001 Men*et"="i Dosen -em,im,in& I .Dr) ;am,an& -oernomo, M7), #rh) <I-) 130 ?01 131 Men*et"="i Dosen Ter ait II .Dr) Bi#=iati, M7), #rh) <I-) 131 3?? 332 Men*et"="i Dosen -em,im,in& II .Ira 7ari @"#ania*anti, M7), #rh) <I-) 132 1?2 342 Men*et"="i Dosen Ter ait III .@"ni -ri*an#ani, Apt, 7p)!R7) <I-) 1A?30221200?012001

Pe12er),3 Fr,97) A)r Kul)- Bu,h Jeru9 Ke:ro9 (Citrus nobilis Lour.) Terh,*,: K,*,r N)-ro4e3 Ure, D,r,h D,l,1 Seru1 D,r,h Me38)(Mus musculus) J,3-,3

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')*+),-).

$)

De/) Ro/)-,.

0)

B,12,34 Poer3o1o.

5)

Ir, S,r) (u*,3),6,3-).

1)

Departemen Anatomi Veteriner,

Mahasiswa, 3) Departemen Anatomi Veteriner, Klini Veteriner !a "ltas Ke#o teran Hewan $ni%ersitas Airlan&&a A27-r,9

2)

4)

Departemen

Ter#apat ,an*a an#"n&an *an& ,erman'aat #i#alam "lit =er" ,"ah Citrus nobilis lo"r) *ait" ,er"pa al aloi#, 'la%onoi#, poli'enol #an hesperi#in *an& #apat ,er'"n&si se,a&ai anti'ertilitas) 7elain it", ter#apat p"la an#"n&an *an& mer"&i an ,er"pa saponin *an& m"n& in #apat mer"sa &in=al *an& #apat mempen&ar"hi e s resi "rea #i #alam #arah) T"="an penelitian ini "nt" men&etah"i pen&ar"h pem,erian 'ra si air "lit ,"ah =er" epro . Citrus nobilis Lo"r)) terha#ap a#ar nitro&en "rea #arah #alam ser"m #arah men(it . Mus musculus) =antan) -enelitian ini men&&"na an sampel 24 e or men(it =antan #i,a&i se(ara a(a #alam 4 perla "an #an masin&C masin& 2 "lan&an) Masin&Cmasin& perla "an .-1/ 40 m&1 & ;;, -2/ 20 m&1 & ;;, -3/ 30 m&1 & ;;) #i,eri an 'ra si air Citrus nobilis Lo"r) se(ara oral 1 ml selama 34 hari, e("ali pa#a elompo ontrol *an& #i,eri perla "an #en&an aquadest 1 ml selama 34 hari) -enelitian ini #ianalisis men&&"na an Ran(an&an A(a Len& ap .RAL)) Dilan="t an #en&an $=i ;e#a <*ata Ter e(il .;<T) #en&an tara' si&ni'i an 48 "nt" men&etah"i perla "an *an& ,er,e#a) Hasil #ari penelitian ini men"n=" an a#ar nitro&en "rea #arah ter#apat per,e#aan *an& n*ata pa#a eempat elompo perla "an .p90)04), *ait" ter=a#i penin& atan *an& n*ata pa#a elompo perla "an -1 tetapi masih #alam ,atas har&a normal) Dan ter=a#i per,e#aan ti#a n*ata pa#a perla "an -2 : -3

K,-, Ku38): 'ra si air "lit ,"ah =er" "rea #arah, anti'ertilitas Pe3*,hulu,3

epro .Citrus nobilis Lo"r)), Ka#ar nitro&en

Tanaman >er" mer"pa an tanaman *an& ,er hasiat o,at) 7pesies =er" melal"i ,er,a&ai ma(am penelitian *an& telah #ila " an #i etah"i men&an#"n& ,er,a&ai ma(am ,ahan a ti' #en&an ,er,a&ai ma(am e&"naan) Kan#"n&an terse,"t antara lain ,er"pa %itamin 6, ,er,a&ai ma(am 'la%onoi# serta min*a atsiri .Morris, 2004)) 7alah sat"

spesies tanaman =er" *an& ,an*a #i,"#i#a*a an #an memili i tin& at ons"msi *an& tin&&i #i In#onesia a#alah Citrus nobilis Lo"r) ata" =er" epro .Ashari, 1AA4)) K"lit

,"ah Citrus nobilis Lo"r) men&an#"n& ,er,a&ai ma(am omponen *ait" %itamin A, %itamin 6, limonene, (itral, hesperi#in, #an sen*awa poli'enol *an& termas" inhi,itor hial"roni#ase) Hesperi#in *an& mer"pa an salah sat" 5at *an& ter an#"n& #alam "lit ,"ah Citrus nobilis Lo"r) memp"n*ai ,er,a&ai ma(am a ti'itas ,iolo&is, antara lain se,a&ai antio si#an, antiin'lamasi, antialer&i, hipolipi#emia, serta #apat men&ham,at sintesis ,ahan

prosta&lan#in) Trin&ali .2001), #alam penelitian*a men"n="

an ,ahwa hesperi#in ="&a

memp"n*ai e'e anti an er #en&an men&ham,at a5o+*methane .ADM) *an& mer"pa an pen(et"s an er "s"s pa#a ti "s serta <C,"t*lC<C.4Ch*#ro+*C,"t*l) nitrosamine *an&

mer"pa an pen(et"s an er sal"ran emih pa#a men(it) -a#a penelitian lain, Hesperi#in #apat men(e&ah penetrasi spermato5oa #alam proses 'ertilisasi in vitro .-ra=o&o # ), 2002)) ;er#asar an #ata terse,"t, 7etiawan .2002) mela " an penelitian #en&an men&&"na an 'ra si air "lit ,"ah Citrus nobilis Lo"r) *an& #i,eri an per oral pa#a men(it .Mus musculus) #en&an #osis 100, 200, 300 m&1 & ;;) Hasil penelitian terse,"t men"n=" an ,ahwa "lit ,"ah Citrus nobilis Lo"r) #apat men&a i,at an ham,atan

proses penetrasi spermato5oa arena #apat men&ham,at a ti'itas en5im hial"roni#ase spermato5oa men(it #alam proses #ispersi lapisan cumulus oophorus ovum. Citrus nobilis Lo"r) men&an#"n& hesperi#in *an& mer"pa an salah sat" omponen #ari sen*awa

'la%onoi# #an memili i a ti'itas se,a&ai inhibitor hial"roni#ase *an& ,isa mem,eri an e'e anti'ertilitas apa,ila #i,eri an se(ara terat"r, sehin&&a #apat #i&"na an se,a&ai

,ahan ontrasepsi .Li et al), 1AA?)) <am"n #emi ian, pen&&"naan "lit ,"ah Citrus nobilis Lo"r) se(ara terat"r

se,a&ai ,ahan ontrasepsi perl" #ipertim,an& an arena #alam "lit ,"ah Citrus nobilis Lo"r) ="&a ter an#"n& sen*awa *an& mer"&i an *ait" saponin, *an& #apat men*e,a, an to si #an "rti aria) To sin saponin #i enal #en&an sapoto sin, pa#a onsentrasi *an& ren#ah serin& men*e,a, an hemolisis sel #arah merah .Kam, 1A3A)) 7aponin =i a #i ons"msi #alam wa t" *an& lama #apat men&a i,at an er"sa an =arin&an seperti lesi pa#a &in=al, ne'rosis #an ne rosis t","l"s pro+imal &in=al .E%ans, 1AA2))

Ean&&"an '"n&si &in=al men*e,a, an la=" 'iltrasi &lomer"l"s men"r"n #an men&a i,at an e s resi "rea ter&an&&") $rea #i 'iltrasi oleh &lomer"l"s #an a an #ie s resi an ,ersama "rin .;i=anti, 200A)) -enin& atan <itro&en $rea Darah .;$<) "m"mn*a men"n=" an re#" si pa#a '"n&si &in=al .Horne #an 7wearin&en, 2001))

Men"r"t E"*ton an# Hall .1AA?), -arameter er"sa an '"n&si &in=al #apat #i etah"i #en&an pemeri saan a#ar "rea #alam #arah ata" ser"m .;$<), a#ar reatinin #alam ser"m, .Glomerolus Filtration Rate F E!R), clearence reatinin, #an clearence "rea) -a#a penelitian ini #i&"na an pemeri saan ;$< #en&an alasan erena a#ar ;$< men&&am,ar an eseim,an&an antara pem,ent" an "rea #an ata,olisme protein serta e s resi "rea oleh &in=al .Bah*"ni #an ;i=anti, 2002)) E s resi "rea mer"pa an '"n&si &in=al *an& terpentin& .Herper, 1A33) Ma a ,er#asar an "raian terse,"t #iatas, se,el"m #i&"na an se,a&ai o,at, ,ahan o,at *an& #i&"na an perl" #ila " an "=i eamanan s"at" ,ahan o,at, arena men"r"t Dep es R)I .1AA4) "nt" men&etah"i em"n& inan a#an*a e'e to si setelah pemaparan 5at imia se(ara ,er"lan& #alam =an& a wa t" tertent" perl" #ila " an pen&"=ian sampin&n*a "nt" or&an tertent") $=i terse,"t #apat linis, #an pemeri saan

sehin&&a #apat #i etah"i e'e

#ila " an #en&an ,e,erapa parameter hematolo&i, ,io imia

histopatolo&i) Dleh arena it", perl" #ila " an penelitian men&enai per",ahan a#ar <itro&en $rea Darah #alam 7er"m Darah pa#a men(it =antan *an& #ia i,at an oleh pem,erian 'ra si air "lit ,"ah Citrus nobilis Lo"r) *an& #apat ,er'"n&si se,a&ai ,ahan ontrasepsi, se,a&ai pers*aratan e'e Me-o*e Pe3el)-),3 -em,"atan 'ra si air "lit ,"ah =er" epro . Citrus nobilis Lo"r)) #ila sana an eamanan o,at)

#i La,oratori"m !ito imia Departemen Ilm" ;ahan Alam !a "ltas !armasi $ni%ersitas Airlan&&a) -erla "an #ila sana an #i an#an& "nit hewan (o,a Kamp"s 6 !a "ltas

Ke#o teran Hewan $ni%ersitas Airlan&&a

em"#ian #ilan="t an #en&an pemeri saan a#ar <itro&en $rea

sampel #arah men(it (Mus musculus) =antan "nt" men&etah"i

Darah #alam 7er"m Darah men(it (Mus musculus) =antan *an& #ila " an #i La,oratori"m -atolo&i Klini Airlan&&a) Hewan per(o,aan *an& #i&"na an a#alah men(it (Mus musculus) =antan 24 e or *an& ,er"m"r 2 ,"lan #en&an ,erat ,a#an rataCrata 30 &) 7ampel *an& #i&"na an a#alah ser"m #arah 0,01 ml) ;ahan *an& #i&"na an ter#iri #ari/ 'ra si air "lit ,"ah Citrus nobilis Lo"r), lar"tan <aDH 2< #an lar"tan H6l 2< "nt" pem,"atan 'ra si air "lit ,"ah Citrus nobilis Lo"r), pa an a*am *an& ,er,ent" crumble "nt" pa an men(it, 7er"m1plasma =ernih, $rease 40)000 $1L, Buffer EDTA pH 2,4, Rea&en I, Rea&en II) Per7),:,3 He;,3 Co2, 7e,el"m penelitian #ila " an, men(it =antan (Mus musculus) #ia#aptasi an pa#a an#an& selama ? hari) Kem"#ian #i,a&i se(ara a(a men=a#i 4 elompo *ait" sat" elompo ontrol .-0) #an ti&a elompo perla "an .-1, -2, #an -3), masin&Cmasin& Veteriner !a "ltas Ke#o teran Hewan $ni%ersitas

perla "an #en&an 2 "lan&an) 7e,el"m #i,eri perla "an hewan (o,a terse,"t #ia#aptasi an #en&an #i,eri pa an a*am *an& ,er,ent" crumble #an min"m se(ara ad libitum) 7etelah #ia#aptasi an hewan (o,a m"lai #i,eri an aquadest #an sol"sio 'ra si air "lit ,"ah Citrus nobilis Lo"r) per oral melal"i son#e selama 34 hari) Pel,97,3,,3 Pe3el)-),3 7etelah men(it #ia#aptasi an selama ? hari, em"#ian #i elompo empat perla "an *an& ,er,e#a/ 1) 2) -0 -1 / Kontrol #en&an Aquadest steril se,an*a 1 ((1e or -D, / 40 m&1 & ;; se,an*a 1 ((1e or -D, an men=a#i

3) 4)

-2 -3

/ 20 m&1 & ;; se,an*a 1 ((1e or -D, / 30 m&1 & ;; se,an*a 1 ((1e or -D,

Dosis terse,"t #i atas men&a(" pa#a #osis #alam penelitian B"lan, .2010), *an& #i#asar an pa#a penelitian 7etiawan .2002) ,ahwa #en&an #asar #osis 100 m&1 & ;; ti#a ter=a#i 'ertilisasi) -em,erian #ila " an setiap hari per oral #en&an son#e selama 34 hari) -a#a hari eC32 setelah perla "an, #ila " an pen&am,ilan #arah melal"i =ant"n& #en&an (ara memas" an disposable syrin e 1 ml) 7e,el"mn*a terle,ih #ah"l" men(it em"#ian

#i or,an an) Darah #itamp"n& #alam ta,"n& rea si tanpa anti oa&"lan

#isentri'"&asi #in&in #en&an e(epatan 4000 rpm selama 10 menit) 7er"m *an& #iperoleh #ari hasil sentri'"&asi #i&"na an se,a&ai sampel "nt" pemeri saan a#ar <itro&an $rea Darah) R,38,34,3 Pe3el)-),3 *,3 A3,l)7)7 D,-, Men&&"na an Ran(an&an A(a Len& ap .RAL) #en&an empat perla "an #an enam "lan&an) Dianalisis #en&an "=i ! ata" Analysis !f "ariant .Ano%a)) >i a ter#apat pen&ar"h *an& n*ata, ma a perhit"n&an #ari masin&Cmasin& perla "an a an #ilan="t an #en&an $=i ;e#a <*ata Ter e(il .;<T) #en&an tara' si&ni'i an 48 .K"srinin&r"m, 2003)) H,7)l *,3 Pe12,h,7,3 Ta,el 4)1 RataCrata #an 7impan&an ;a " Ka#ar <itro&en $rea Darah .;$<) Men(it .Mus musculus) setelah perla "an) PERLAKUAN P0 P$ P# P! Keteran&an / 7"pers rip *an& n*ata K,*,r (BUN) (14<*l) (= > SD) 12,A03 G 0,340A, 12,?4? G 1,1332,( 14,04 G 1,3332a 12,212 G 2,2333,( *an& ,er,e#a pa#a olom *an& sama men"n=" an per,e#aan

-erhit"n&an statisti #en&an "=i ! terha#ap a#ar <itro&en $rea Darah .;$<) men"n=" an ! hit"n& 3,44 le,ih ,esar #ari ! ta,el 3,10 pa#a tara' si&ni'i an 48) ;erarti

empat elompo perla "an ter#apat per,e#aan *an& n*ata .p 9 0,04) terha#ap a#ar <itro&en $rea Darah .;$<)) 7elan="tn*a #ila " an "=i ;<T #en&an tara' 48) -a#a "=i ;<T 48 #it"n=" an ,ahwa elompo perla "an - 1 mem,eri an hasil tertin&&i #an eti&a elompo perla "an *ait" - 3, -2 #an -0)

,er,e#a n*ata .p 9 0,04) #en&an 7e#an& an antara

elompo perla "an - 0, -2, -3 ter=a#i per,e#aan *an& ti#a ,er,e#a

n*ata antara eti&an*a) ;er#asar an hasil perhit"n&an statisti terha#ap a#ar <itro&en $rea Darah .;$<) eempat elompo perla "an #i#apat an rataCrata "nt" perla "an pem,erian 'ra si air Citrus nobilis Lo"r) 30 m&1 & ;; .-3) se,esar 12,A03 .m&1#l), perla "an pem,erian 'ra si air Citrus nobilis Lo"r) 20 m&1 & ;; .-2) se,esar 12,?4? .m&1#l), perla "an pem,erian 'ra si air Citrus nobilis Lo"r) 40 m&1 & ;; .-1) se,esar 14,04 .m&1#l) #an perla "an ontrol .- 0) se,esar 12,212 .m&1#l)) ;er#asar an an ter#apat per,e#aan *an& n*ata

perhit"n&an #en&an men&&"na an $=i ! men"n=" #iantara eempat perla "an)

-a#a elompo perla "an -1 .#osis ter e(il) #ihasil an a#ar ;$< palin& tin&&i, se,ali n*a pa#a elompo perla "an #en&an #osis *an& le,ih tin&&i .- 2 #an -3) a#ar ;$< ti#a ,er,e#a se(ara n*ata #en&an ontrol .- 0)) Hal ini m"n& in #ise,a, an arena ser"m #arah *an& lisis a i,at esalahan pa#a saat sentri'"&asi sehin&&a men*e,a, an enai an a#ar ;$< *an& ,er,e#a n*ata #iantara empat elompo perla "an) 7elain it" a#ap"n halChal lain *an& #apat mempen&ar"hi enai an a#ar ;$< *ait" per#arahan pa#a &astrointestinal, #ehi#rasi, menin& atn*a inta e protein, menin& atn*a ata,olisme protein, in'e si sistemi , #an terapi men&&"na an orti osteroi# .Kher, 1AA2)) Dehi#rasi ata" sho #apat ,era i,at ="mlah "rea *an& #i el"ar an a an men"r"n sehin&&a a#ar "rea #alam sir "lasi #arah men=a#i menin& at .Bah="ni #an ;i=anti, 2002)) Mes ip"n

hasil men"n="

an -1 ,er,e#a n*ata terha#ap elompo perla "an lain .- 0, -2 #an -3),

tetapi a#ar ;$< masih #alam ,atas har&a normal) Har&a normal ;$< *ait" ,er isar 11,A H 23,0 m&1#l) Ka#ar ;$< setelah pem,erian 'ra si air "lit ,"ah Citrus nobilis Lo"r) ,er isar antara 12,?4 H 14,04 m&1#l) Den&an ata lain, 'ra si air "lit ,"ah Citrus nobilis Lo"r) ti#a menim,"l an &an&&"an pa#a '"n&si &in=al) Hasil terse,"t #i#" "n& #en&an laporan A&"stina .2010) *ait" ,ahwa se(ara histopatolo&i pem,erian 'ra si air "lit ,"ah Citrus nobilis Lo"r) #en&an #osis 40 m&1 & ;;, 20 m&1 & ;; #an 30 m&1 & ;; relati' aman) -er",ahanCper",ahan *an& ter=a#i pa#a &am,aran mi ros opi &in=al men(it . Mus musculus) =antan *ait" on&esti #an #e&enerasi masih #alam tahap rin&an #an ,ersi'at re%ersi,el =i a pen*e,a,n*a #ihilan& an) Ke7)1:ul,3 Dapat #isimp"l an ,ahwa -em,erian 'ra si air "lit ,"ah Citrus nobilis Lo"r) ti#a menin& at an a#ar <itro&en $rea Darah arena a#ar ;$< masih #alam ,atas har&a normal .11,AC23 m&1#l), #en&an hasil tertin&&i #i#apat pa#a perla "an - 1 .14,04 m&1#l)) D,?-,r Pu7-,9, A&"stina, -) 2010) -en&ar"h -em,erian !ra si Air K"lit ;"ah >er" Kepro . Citrus nobilis Lo"r)) Terha#ap Eam,aran Histopatolo&i Ein=al Men(it (Mus musculus) >antan) 7 ripsi) !a "ltas Ke#o teran Hewan $ni%ersitas Airlan&&a) 7"ra,a*a)

Ashari, 7) 1AA4) Horti "lt"ra Aspe ;"#i#a*a) -ener,it $ni%ersitas In#onesia .$IC-ress)) >a arta) 310C312)

;i=anti, R) 200A) ;ahan A=ar -atolo&i Klini Veteriner, Kimia Klini Veteriner) E#isi -ertama) Departemen Ke#o teran Dasar Veteriner) !a "ltas Ke#o teran Hewan $ni%ersitas Airlan&&a) 7"ra,a*a

Dep es R)I) 1AA4) -rose#"r Dperasional ;a " $=i To sisitas) -"sat -emeri saan D,at #an Ma anan BHD 6olla,oratin& 6entre 'or I"alit* Ass"ran(e Essential Dr"&s) Dep es R)I) >a arta

E%ans) 1AA2) !arma(o&nos*) 13 E#ition) ;aillierre Tin#all) -hila#elpia) Lon#on) 430C 434)

E"*ton, A)6) an# Hall, >)E) 1AA?) !isiolo&i Ke#o teran) E#isi A) -ener,it ;" " Ke#o teran E6E) >a arta) 41?C430) Herper, H)A) 1A33) Re%iew o' ;io(hemistr*) Diter=emah an oleh A#=i Dharma) 6V) E6E) -ener,it) ;" " Ke#o teran) >a arta) 43?C441)

Horne #an 7wearin&en) 2001) Keseim,an&an 6airan, Ele trolit, #an Asam ;asa) E#isi 2) ;" " Ke#o teran EE6) >a arta) 33, 42)

Kam, D)<) 1A3A) JatCJat To si *an& 7e(ara Alamiah A#a pa#a ;ahan Ma anan <a,ati) 6ermin D"nia Ke#o teran <o 43) http/11www) al,e)(o)i#) 30 >"li 200A) Kher, K)K) 1AA2) E%al"ation o' Renal !"n(tion) 6lini(al -e#iatri( <ephrolo&*) <ew @or / M(ErawCHill) 3C22) K"srinin&r"m, R7) 2003) ;" " A=ar -eran(an&an -er(o,aan) !a "ltas Ke#o teran Hewan $ni%ersitas Airlan&&a) -ener,it Dani A,a#i) 7"ra,a*a)

Li, M)B), AI), @"#in, V), Voort) K), 7a,e"r, -), -rima o'', an# >)B) 1AA?) Inhi,ition o' Mon e* 7perm H*al"roni#ase A(ti%it* an# Heterolo&o"s 6"m"l"s -enetration ,* !la%onoi# In/ ;iol) Repor#), 42/2, 1333C133A Morris, R), 2004) Citrus spp) http/11www)p'a')or&1#ata,ase1plants)phpK(itr"sLspp) 30 ="ni 2010 -ra=o&o, D)E)B), Bi#=iati, #an M"l*a#i, T) 2002) -en&ar"h !ra si -oli'enol Een#ar"ssa %"l&aris <ees pa#a -en"r"nan a ti%itas H*aloroni#ase 7permato5oa Men(it melal"i $=i !ertilisasi #n vitro. Dalam/ Laporan -enelitian Ilm" -en&etah"an

Dasar) Lem,a&a -enelitian $ni%ersitas Airlan&&a) !a "ltas !armasi $ni%ersitas Airlan&&a) 7"ra,a*a 7etiawan, D)A) 2002) E'e E stra K"lit ;"ah Citrus nobilis Lo"r Terha#ap An& a !ertilitas) !a "ltas !armasi $ni%ersitas Airlan&&a) 7"ra,a*a)

Trin&ali, 6) 2001) ;ioa(ti%e 6ompo"n# 'rom <at"ral 7o"r(es) Lon#on/ Ta*lor ann !ran(is In() 124C124 Bah="ni, R)7), #an ;i=anti, R) 2002) $=i E'e 7ampin& !orm"la -a an Komplit Terha#ap !"n&si Hati Dan Ein=al -e#et 7api !riesian Holstein) >) Ke#o teran Hewan Vol) 22 .3)/ 1?4 H 1?3