TIU BAN 4. HA SINH V CC HP CHT T NHIN BIN Session 4.

Biochemistry and Marine natural products

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ALKYL-GLYXERIT T SINH VT BIN - MT HP CHT THIN NHIN NHIU TIM NNG HOT TNH SINH HC Phm Quc Long1,*, Chu Quang Truyn1, ng Th Phng Ly1, Phm Minh Qun1, Hoang Thanh Huong1, N.A. Latyshev2, S.P. Kasijanov2 1 . Vin Ha hc cc Hp cht thin nhin 2 . Vin Sinh vt bin, Phn vin Vin ng - Vin Hn Lm Khoa hc Lin bang Nga Alkyl-glyxerit tn ti di dng ete ca glyxerol l lp hot cht c bit c mt trong nhiu sinh vt bin, c bit trong thnh phn cht bo ca gan c mp, c ui vng bin su. Cu trc ho hc, phn b, sinh tng hp ca hot cht c th ny c xem xt. Cc hot tnh sinh hc tim nng i vi h min dch, iu tr ung th, tng kh nng hot ng tinh trng v khng sinh, khng khun cng c tho lun. T kha: Alkyl-glycerit, Cu trc ha hc, Phn b, Sinh tng hp, Hot tnh sinh hc. ALKYL-GLYCERIDE FROM MARINE ORGANISMS A HIGH POTENTIAL NATURAL BIOACTIVE COMPOUND Pham Quoc Long1, Chu Quang Truyen1, Dang Thi Phuong Ly1 Pham Minh Quan1, Hoang Thanh Huong1 N.A. Latyshev2, S.P. Kasijanov2 1 . Institute of Natural Products Chemistry, 18 Hoang Quoc Vit Str., Cau Giay, Ha Noi, Viet Nam 2 . A.V Zhirmunsky Institute of Marine Biology - FEB, RAS * . E-mail: mar.biochem@fpt.vn Alkyl-glyceride exits in the ether form of glycerol - the bioactive compound present in many marine organisms, especially in a live fat composition of sharks and rays from the deep sea. The chemical structure, distribution, biosynthesize of this bioactive compound had been studied. The potential biological activity to the immune system, cancer treatment, improving of sperm activity and antibiotic, anti-bacterial activities had also been discussed. Key words: Alkyl-glyceride, Chemical structure, Distribution, Biosynthesize, Biological activity.

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C TNH TETRODOTOXIN C HNG BABYLONIA AREOLATA (LINK, 1807) NUI BNG THC N CH BIN T C NC C o Vit H, Phm Xun K, Nguyn Phng Anh, Phan Bo Vy, ng Quc Minh Vin Hi dng hc tm hiu kh nng tch ly c t tetrodotoxin nhuyn th, c hng Babylonia areolata khong mt thng tui c nui bng thc n ch bin t gan v c c nc Chm cam Torquigener gloerfelti vi liu c 33,4 MU/g trong thi gian 05 thng. L c hng c cung cp thc n ch bin t c nc c c tc sinh trng thp hn so vi l i chng c cho n bng thc n cng nghip. c tnh TTX trong c hng gia tng lin tc theo thi gian nui (R2 = 0,92). Sau 02 thng th nghim, TTX trong c hng t gi tr c tnh tng ng ngng an ton thc phm i vi c t ny (10 MU/g) v gp khong 2,3 ln thi im kt thc th nghim (sau 05 thng nui). Mt khc, sau khong thi gian ny, c n 57,1% s c th c hng cha c tnh TTX vt ngng an ton v c bit c nhng c th biu hin c tnh kh cao (>50 MU/g). Nhng kt qu t c cho thy c hng c kh nng tch ly c t TTX cao t ngun thc n c cha TTX. Do , s dng c nc c lm thc n trong nui thy sn l khng chc chn bo m an ton thc phm. T kha: TTX, c tnh, c hng, Babylonia areolata. TETRODOTOXIN TOXICITY IN THE SPOTTED BABYLON, BABYLONIA AREOLATA (LINK, 1807) FED BY TOXIC PUFFER Dao Viet Ha*, Pham Xuan Ky, Nguyen Phuong Anh, Phan Bao Vy, Dang Quoc Minh Institute of Oceanography, 01 Cau Da, Vinh Nguyen, Nha Trang City, Viet Nam * . E-mail: daovietha69@gmail.com To clarify the accumulation of tetrodotoxin in mollusc, one-month old spotted Babylon, Babylonia areolata (Link, 1807) was fed on the food pellet made from the muscle and liver of the toxic puffer fish Torquigener gloerfelti for 05 months under rearing condition. The growth rate of the experimental Babylon supplied by toxic puffer was lower than that of the control one fed by commercial food. The toxicity in the experimental spotted Babylon increased continuously during the rearing time (R2 = 0.92). After rearing for two months, the TTX toxicity of the spotted Babylon reached the safety limit (10 MU/g), then 2.3 times higher in the end of experiment (05 months). In addition, after this period, 57.1 % individuals exhibited the TTX toxicity higher than 10 MU/g, in which some of them showed relatively high toxicity (>50 MU/g). The results indicate that the spotted Babylon can highly accumulate TTX from food supply containing TTX. Therefore, using toxic puffers as diets for aquacultural species cannot be a safety way. Key words: TTX, Toxicity, Spotted Babylon, Babylonia areolata. 86

NGHIN CU NH GI CHT LNG MC NG LOLIGO CHINENSIS GRAY TI VNG O C T on Lan Phng1, Nguyn Th Thu1, Cm Th nh1, Trn Th Dung2, Phm Quc Long1 1 . Vin Ha hc cc Hp cht thin nhin 2 . Cng ty TNHH T vn v dch v thy sn Hng Ph Mc ng Loligo chinensis Gray l mt trong nhng loi mc cho sn lng ln nht v c gi tr xut khu cao. Chnh v vy chng ti la chn i tng ny nghin cu, nh gi cht lng nguyn liu nhm nng cao gi tr s dng ca chng. Trong bi bo ny, chng ti nghin cu, nh gi hm lng lipit tng, thnh phn cc axit bo, hm lng protein, thnh phn axit amin cng mt s ch tiu ho l khc c trong loi mc ng L. chinenis thu c ti vng o C T. Cc kt qu thu nhn c cho thy loi mc ny c gi tr dinh dng rt cao, trong ng ch l hm lng DHA ln ti 33,502 % v cha n 09 axit amin thit yu vi hm lng cao v chnh lch t. T kha: Loligo chinensis, o C T, Gi tr dinh dng, DHA, Axit amin. EVALUATING THE QUALITY OF THE SQUID LOLIGO CHINENSIS GRAY AT CO TO ISLAND WATER Doan Lan Phuong1,*, Nguyen Thi Thu1, Cam Thi Inh1, Tran Thi Dung2, Pham Quoc Long1 1 . Institute of Natural Products Chemistry, 18 Hoang Quoc Viet Str., Cau Giay, Ha Noi, Viet Nam 2 . Hung Phu consulting and seafood service limited liability company * . E-mail: doanlanphuong75@yahoo.com Gray squid (Loligo chinensis) is one of the squid species which has high productivity and also high commercial value. Therefore, it is chosen the object to study for evaluating the material quality to improve the value of their use. In this paper, data of lipid content, fatty acid composition, protein, amino acid composition and some other chemical, physical criteria in the squid L. chinensis collected at Co To island water is presented. The obtained results showed that this species has very high nutritional value, with level of DHA up to 33,502% and contains nine essential amino acids at high concentration and low deviations, notably. Key words: Loligo chinensis, Co To island, Nutrional value, DHA, Amino acids.

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NGHIN CU THNH PHN SINH HA CA MT S LOI SAO BIN VIT NAM on Lan Phng1, Nguyn Th Thu1, Trnh Th Thu Hng1, Phm Minh Qun1, Trn Th Thy1, Manobjyoti Bordoloi2, Phm Quc Long1 1 . Vin Ha hc cc Hp cht thin nhin 2 . Vin Khoa hc v Cng ngh ng Bc n (NEIST) Ngun li bin Vit Nam l mt trong nhng yu t quan trng i vi s nghip xy dng v pht trin t nc trong thi k hin nay. Tuy nhin ngun li ti nguyn thin nhin ny vn cha c s dng mt cch hiu qu nht. Vi mc ch to c s khoa hc c th nh hng khai thc hiu qu ngun ti nguyn sinh vt bin Vit Nam, chng ti chn 05 mu Sao bin (Archaster typicus, Acanthaster planci, Linckia laevigata, Anthenea aspera, Culcita novaeguineae) thuc ngnh Da gai (Echinodermata) nghin cu. Bi bo ny trnh by qu trnh phn tch v kt qu nghin cu thnh phn lipit, axit bo, protein v axit amin t cc loi sao bin ny. T kha: Sao bin, Lipit, Axit bo, Protein, Axit amin. BIOCHEMICAL COMPOSITION OF SOME STARFISH SPECIES FROM VIET NAM Doan Lan Phuong1,*, Nguyen Thi Thu1, Trinh Thi Thu Huong1, Pham Minh Quan1, Tran Thi Thuy1, Manobjyoti Bordoloi2, Pham Quoc Long1 1 . 18 Hoang Quoc Viet Str., Cau Giay, Ha Noi, Viet Nam 2 . North East Institute of Science and Technology * . E-mail: doanlanphuong75@yahoo.com The potential marine resource from Vietnam sea is one of important factors for the country construction and development in current period. However, marine resources havent been exploited effectively in Vietnam. With the objective to provide a scientific background for effective exploitation of marine resources, five starfish species (Archaster typicus, Acanthaster planci, Linckia laevigata, Anthenea aspera and Culcita novaeguineae) were chosen for our study. In this paper, we reported the analysis process and the study results of lipid, fatty acids, protein and amino acid from these starfish. Key words: Starfish, Lipid, Fatty acids, Protein, Amino acid.

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HOT TNH KHNG VI SINH VT CA MT S VI NM PHN LP VNG VEN BIN VIT NAM
Nguyn nh Luyn, L Mai Hng*, Trn Th Hng H, Trn Th Nh Hng, Phm Quc Long Vin Ha hc cc Hp cht thin nhin, Vi sinh vt bin l mt trong s cc ngun ti nguyn cha cc cht c hot tnh sinh hc mi. Trong thi gian tham gia chuyn kho st ven bin ca tu Academic Oparin nm 2010, hng trm mu vt ven bin bao gm bn y, c bin, hi sm, hi min, ng vt thn mm, bn bin v xc thc vt ph du c thu thp cho phn lp vi sinh vt. Trong khun kh bo co ny, chng ti trnh by cc thng tin v 50 trong s cc chng vi nm phn lp c, danh sch, mt s c im hnh thi nui cy v hot tnh khng vi sinh vt kim nh trn 8 chng kim nh. Trong s 50 chng phn lp, c 11 chng( chim 22%) biu hin hot tnh khng sinh khng t nht 3 trn tng s 8 chng vi sinh vt kim nh tin hnh th nghim; 6 chng ( chim 12%) khng 4 trn 8 VSV kim nh; 5 chng (chim 10%) khng 5 chng VSV kim nh tr ln trn 8 chng th nghim. Cc chng c hot tnh cao c chn lc cho nghin cu ha hc theo nh hng hot tnh sinh hc cc bc tip theo. T kha: Vi nm; Hot tnh khng vi sinh vt; Vi sinh vt bin. THE ANTIMICROBIAL ACTIVITY OF SOME MICROFUNGI ISOLATED FROM VIETNAMESE COASTAL WATERS Nguyen Dinh Luyen, Le Mai Huong*, Tran Thi Hong Ha, Tran Thi Nhu Hang, Pham Quoc Long * : E-mail: lehuong00@gmail.com Institute of Natural Products Chemistry, 18 Hoang Quoc Viet Str., Cau Giay, Ha Noi, Viet Nam * : E-mail: lehuong00@gmail.com Marine microorganisms are considered as one of the potential resources for new bioactive compounds. In the trip go along ship Academik.Oparin in 2010, a hundred of samples including bottom sediment, seaweed, sea urchin, mollusk, sea cucumber, sea slug, dead wood, sponge, and other species were collected for isolation of microorganisms. In this paper we are reporting the list of 50 isolated microfungi, their cultural characteristics and antimicrobial activity against eight tested control microorganisms. Among 50 microfungi strains, 11 strains (22%) exhibited antimicrobial activity against at least 3 out of 8 control microorganisms; other 6 (12%) against 4 of 8 control organisms; 5 strains (10%) against 5 out of 8 control microorganisms up. Some strong active strains have been choosing for bioguided chemical fractionation. Key words: Microfungi, Antimicrobial activity, Marine microorganisms.

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THNH PHN SINH HA CA S HUYT ANADARA GRANOSA (L.) M LNG C, TNH THA THIN-HU
Ch Th Cm H1, Phan Th Thu Hng2, Nguyn Minh Tr1 1 . Trng i hc Khoa hc Hu 2 . S Nng nghip v Pht trin Nng thn, Tha Thin-Hu Trong nhng nm gn y, sn phm S huyt Anadara granosa (L.) nui ti m Lng C, tnh Tha Thin Hu em li cho ngi dn vng ny mt ngun li kinh t kh ln ci thin i sng v nng cao thu nhp. Mt s kt qu nghin cu trnh by trong bi bo ny cho thy cc thnh phn ha hc nh protein, lipid, ng tng s v hm lng tro trong tht S huyt tng i cao, c bit l cc mu vt S huyt trng thnh. S c mt ca 09 axit amin khng thay th nh methionine, threonine, lysine, isoleucine, leucine, valine, arginine, histidine v phenylalanine l mt u im ca i tng ny. Mt khc, trong tht ca S huyt c cha hm lng kh cao cc nguyn t vi lng cn thit cho qu trnh trao i cht nh Cu, Fe, Zn, Mn v Ca. Kt qu nghin cu ny ch ra rng S huyt l i tng l tng s dng nh mt loi thc phm, dc phm cho con ngi. T kha: S huyt, Anadara granosa, Thnh phn ha hc, Axit amin, Khong vi lng. BIOCHEMICAL COMPOSITION OF THE BLOOD COCKLE ANADARA GRANOSA (L.) IN LANG CO LAGOON, THUA THIEN HUE PROVINCE Che Thi Cam Ha1,*, Phan Thi Thu Hong2, Nguyen Minh Tri1 1 . Hue University of Science, 77 Nguyen Hue St. Hue City 2 . Department of Agriculture and Rural Development Thua Thien Hue * . E-mail: chethicamha@yahoo.com In recent years, the blood cockle Anadara granosa (L.) farming products at Lang Co Lagoon, Thua Thien Hue Province is considered as a important seafood production to increase incomes for local fishermen. In this paper, the result showed that the biochemical components such as proteins, lipids, sugars and total ash content of the A. granosa meat are relatively high. Importantly, nine essential amino acids such as methionine, threonine, lysine, isoleucine, leucine, valine, arginine, histidine and phenylalanine are presented in A. granosa meat. Also, some trace elements necessary for metabolic processes such as Cu, Fe, Zn, Mn and Ca are detected in the A. granosa meat at high concentration. The studied result indicated that A. granosa could be used not only as a high nutrient food but also as potential medicine. Key words: Blood cockcle, Anadara granosa, Biochemical composition, Acid amin, Trace elements.

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TIM NNG S DNG LECTIN T RONG KAPPAPHYCUS ALVAREZII L nh Hng1,2, Kanji Hori2 . Vin Nghin cu v ng dng Cng ngh Nha Trang 2 . Graduate School of Biosphere Science, Hiroshima University, Japan
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Rong Kappaphycus alvarezii l loi rong kinh t khng ch dng lm thc phm cho con ngi m cn lm nguyn liu sn xut carrageenan, v c nui trng rng ri nhiu nc vng nhit i. Trong khun kh nghin cu ny, lectin t rong K. alvarezii c tinh ch nh gi cc tnh cht ha sinh v hm lng ca chng. Kt qu cho thy rng loi rong ny cha ba lectin, c t tn KAA-1, KAA-2, v KAA-3, c chung c tnh c ch khng nguyn, trnh t 20 acid amino u tn cng N (N-terminus) v khi lng phn t tng ng, nhng li khc nhau v mt hm lng. Lectin t rong K. alvarezii c kh nng c ch s ly nhim ca cc dng virus cm khc nhau nng nanomolar, v ngn cn virus xm nhp vo t bo ch. Cc kt qu nghin cu ch ra rng lectin t rong K. alvarezii s l ngun thuc th khng virus mi. T kha: Kappaphycis alvarezii, Lectin, Vi-rt. APPLICATION POTENTIAL OF LECTIN FROM RED ALGA KAPPAPHYCUS ALVAREZII Le Dinh Hung1,2,*, Kanji Hori2 . Institute of Technology Research and Application, 2A, Hung Vuong Str., Nha Trang City, Khanh Hoa province, Vietnam 2 . Graduate School of Biosphere Science, Hiroshima University, Kagamiyama 1-4-4, Higashi Hiroshima 739-8528, Japan * . E-mail: ledinhhungims@yahoo.co.uk
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The red alga Kappaphycus alvarezii not only is economically important as an edible algae but also as a source of carrageenan, and has been extensively cultivated in several tropical countries. In this study, lectins from K. alvarezii were isolated and characterized for determinating of their biochemical properties and contents. The results showed that K. alvarezii contained the three lectins named KAA-1, KAA-2 and KAA-3, which shared the hapten-inhibition profile of hemagglutination, 20 N-terminal amino acid sequence, and equivalent molecular mass, but differed in their yields. Lectins from K. alvarezii inhibited infection of various influenza strains with low nanomolar levels, and prevented virus entry into host cells. These results indicate that lectins from K. alvarezii would be useful as a novel antiviral reagent. Key words: Kappaphycis alvarezii, Lectin, Virus.

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BA H THNG HOC MN GY TIT KCH DC T NO C CHM LATES CALCARIFER (BLOCH, 1790) Pham Xuan Ky1, Dao Viet Ha1, Masafumi Amano2, Noriko Amiya2 1 . Vin Hi dng hc 2 . Khoa Sinh hc bin, i hc Kitasato, Nht Bn Kt qu rpHPLC cho thy cht chit ca no c chm c thi gian lu tng t vi cc peptides tng hp sGnRH, cGnRH-II, v sbGnRH. Trong no, hm lng cGnRH-II v sGnRH cao, trong khi hm lng sbGnRH u th tuyt i tuyn yn. Cc th t bo sGnRH phn b dc theo vng bng ca hnh khu gic, vng gia hnh khu gic v gian no. Cc t bo cGnRH-II ch nm np no gia v t bo sbGnRH c tm thy vng trc th gic. Cc th si sGnRH phn b trong ton b no, c bit dy no trc. Cc th si cGnRH-II c pht hin nhiu phn no vi mt cao no gia, trong khi cc th si sbGnRH tp trung vng trc th gic- i yn nga hng v tuyn yn. Trong tuyn yn, cc th si ny c mt phn sau v xm nhp phn gia ca tuyn yn. Cc kt qu khng nh s tn ti ca nhiu dng GnRH vi cc vai tr sinh l khc nhau trong no c chm. Trong , sbGnRH c xem l yu t iu khin chc nng ca tuyn yn, sGnRH v cGnRH-II c th hot ng nh l cc yu t dn truyn v iu ha thn kinh no loi c ny. T kha: GnRH, No, Tuyn yn, C chm. THREE GNRH FORMS IN BRAIN AND PITUITARY OF ASIAN SEABASS, LATES CALCARIFER (BLOCH, 1790)
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Ky Xuan Pham1, *, Ha Viet Dao1, Masafumi Amano2, Noriko Amiya2 . Institute of Oceanography, 01 Cau Da, Vinh Nguyen, Nha Trang City, Viet Nam 2 . School of Marine Biosciences, Kitasato University, Kanagawa 252-0373, Japan * . E-mail: kyjapan2004@yahoo.com

An extract of Asian seabass brain showed a similar retention time to that of synthetic sGnRH, cGnRH-II, and sbGnRH on rpHPLC. In the brain, cGnRH-II and sGnRH levels were high, whereas sbGnRH was absolutely dominant in the pituitary. sGnRH-ir cell bodies were localized in the ventromedial part of the rostral olfactory bulb, terminal nerve ganglion region, and the transitional area between the olfactory bulb and telencephalon. cGnRH-II-ir cell bodies were only located at the midbrain tegmentum and sbGnRH-ir cell bodies were evident in the preoptic area. sGnRH-ir fibers were distributed throughout the brain, especially abundant in the forebrain. cGnRH-II-ir fibers were found in many parts of the brain with dense in the midbrain, whereas sbGnRH-ir fibers were localized in the preoptic-hypothalamic area, innervating the pituitary. In the pituitary, sbGnRH-ir fibers were profuse in the neurohypophysis and invaded the proximal pars distalis. These results confirm the existence of multiple GnRH forms with different physiological roles in Asian seabass. Among them, sbGnRH is considered to be the best candidate for pituitary regulation, and sGnRH and cGnRH-II can function as neurotransmission or neuromodulation in this species. Key words: GnRH, Brain, Pituitary, Asian seabass. 92

SNG LC HOT TNH KHNG KHUN CA MT S LOI RONG BIN MIN TRUNG VIT NAM Cao Th Thy Hng*, Trn Nguyn H Vy, V Mai Nh Hiu, Nguyn Bch Khoa, Bi Minh L, Trn Th Thanh Vn Vin nghin cu v ng dng Cng ngh Nha Trang Bi bo ny trnh by kt qu sng lc hot tnh khng khun c hai dng vi khun Gram m v Gram dng t cc dch chit methanol v ethyl acetate ca 20 loi rong v rong nu. Hot tnh khng khun c tnh theo ng knh vng khng khun. Dch chit t cc loi rong nu (tr Turbinaria ornata) u c hot tnh khng li chng Pseudomonas alruginosa, trong , hot tnh cao l cc loi rong S. henslowianum, Dictyota dichotoma v D. linearis. Mt khc, hot tnh ca cc dch chit bng ethyl acetate cao hn cc dch chit bng methanol. Dch chit ethyl acethate ca S. crasifolium c hot tnh cao hn c. T kha: Rong, Vi khun gram m, Vi khun gram dng, Hot tnh khng khun, Dch chit. SCREENING FOR ANTIBACTERIAL ACTIVITIES IN SOME MARINE ALGAES FROM THE CENTRAL VIET NAM Cao Thi Thuy Hang*, Tran Nguyen Ha Vy, Vo Mai Nhu Hieu, Nguyen Bach Khoa Nhatrang Institute of Technology Research and Application, 2A Hung Vuong Str., Nha Trang city,Viet Nam * . E-mail: caohang.nitra@gmail.com Methanolic and ethyl acetate extracts from 20 marine algae species belong to Rhodophyceae and Phaeophyceae were screened for their antibacterial activity with both positive and negative bacteria. The antibacterial activity was expressed as zone of inhibition. All most of all extract from brown seaweed (except Turbinaria ornata) showed inhibition activity against Pseudomonas alruginosa with the highest activity are S. henslowianum, Dictyota dichotoma and D. linearis. On the other hand, ethyl acetate extracts exhibited higher activity than methanolic extracts. The most powerful activity was from S. crasifolium extracted by ethyl acetate. Key words: Seaweed, Negative bacteria, Positive bacteria, Antibacterial activity, Extract.

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PHN LP, TUYN CHN V NGHIN CU C IM SINH HC CA VI KHUN BIN SINH BACTERIOCIN NHM PHT TRIN THUC A NNG TRONG NUI TRNG HI SN Nguyn Vn Duy*, Nguyn Th Hi Thanh, L Phng Chung, Phm Thu Thy Vin Cng ngh sinh hc v Mi trng, Trng i hc Nha Trang Nghin cu ny nhm mc tiu tuyn chn v nghin cu c im sinh hc ca cc vi khun bin sinh bacteriocin v xc nh mt s tnh cht ca bacteriocin nhm nh hng ng dng lm thuc a nng (cht khng sinh th h mi, probiotic) trong nui trng thy sn. Kt qu phn lp c 10 chng vi khun t mt s ng vt nui bin c hot tnh sinh bacteriocin khng cc vi khun ch. Chng c nh danh v m t bng cc th nghim ha sinh v gii trnh t on gen 16S rDNA. ng thi, bn, ph hot tnh v c ch hot ng ca dch bacteriocin th c xc nh. Nhng kt qu nghin cu ny cung cp nhng d liu mi v a dng sinh hc, tin ha, sinh l v sinh thi ca cc vi sinh vt bin cng nh tim nng ng dng ca chng trong pht trin thuc a nng phc v nui trng hi sn Vit Nam. T kha: Bacteria, Bacteriocin, Nui trng hi sn, Th nghim ha sinh, Sequence. SCREENING, ISOLATION AND CHARACTERIZATION OF MARINE BACTERIOCIN-PRODUCING BACTERIA FOR THE DEVELOPMENT OF AQUACULTURAL MULTI-FUNCTIONAL DRUGS Nguyen Van Duy*, Nguyen Thi Hai Thanh, Le Phuong Chung, Pham Thu Thuy Institute of Biotechnology and Environment, Nha Trang University, 02 Nguyen Dinh Chieu, Nha Trang City, Viet Nam *: Email: duynv@ntu.edu.vn The present study aims are screening and characterizing marine bacteriocinogenic bacteria as well as determining the properties of bacteriocins for using them as aquacultural multi-functional drugs of anti- and pro-biotics. The results showed that hundreds of bacteria strains were isolated from several aquacultural species such as cobia (Rachycentrum canadum), snubnose pompano (Trachinotus blochii), spiny lobster (Panulirus ornatus) and otter clam (Lutraria philippinarum) in coastal Central Vietnam. Among of them, ten strains exhibited activity against selected target pathogens. Bacteriocinogenic bacteria were then be identified and characterized by biochemical tests and 16S rDNA sequencing. Further, the stability, activity spectrum and mode of action of raw bacteriocins in cell-free supernatants were evaluated. The results of this study provide understanding on the biodiversity, evolution, physiology, ecology of marine microorganisms and their potential applications as multi-functional drugs in aquaculture in Vietnam. Key words: Bacteria, Bacteriocin, Marine aquaculture, Biochemical test, Sequence.

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KHO ST HOT TNH KHNG RI LON LIPID MU CA FUCOIDAN SULFATE HA KHI LNG PHN T THP T RONG NU VIT NAM Nguyn Duy Nht1, Phm c Thnh1, Bi Minh L1, Phan Vn Li2, Nguyn Mnh Cng2, Nguyn Th Minh Ty3, L Xun Sn3, V Ngc Bi3 1 . Vin Nghin Cu V ng Dng Cng Ngh Nha Trang 2 . Vin Ha Hc Cc Hp Cht Thin Nhin; 3. Trng i Hc Nha Trang Nhiu hp cht nhin nhin c tc dng h lipid mu c nghin cu trong c fucoidan. Cng trnh ny c tin hnh nhm nghin cu tc dng n nh lipid mu ca fucoidan sulfate ha khi lng phn t thp, thu c t fucoidan ca rong nu Sargassum polycystum Nha Trang. Nghin cu hot tnh khng ri lon chuyn ha lipid mu ca fucoidan sulfate ha khi lng phn t thp trn chut lang cho thy: Chut c cho n ch bo cao c cc ch s lipid mu tng rt mnh, tuy nhin, nu c n km theo fucoidan sulfate ha khi lng phn t thp (500mg/kg khi lng c th chut) th cc ch s lipis mu khng c g bin i so vi mu trng (chut khng n ch bo cao v khng n fucoidan sulfate ha khi lng phn t thp). T kha: Hot tnh, Ri lon Lipid mu, Fucoidan, Rong nu, Vit Nam. A SURVEY OF ANTI LIPID METABOLISM DISORDERED ACTIVITY OF LOW MOLECULAR WEIGHT SULFATED FUCOIDAN FROM VIETNAMESE BROWN ALGAE Nguyen Duy Nhut1,*, Pham Duc Thinh1, Bui Minh Ly1, Phan Van Lai2, Nguyen Manh Cuong2, Nguyen Thi Minh Tuy3, Le Xuan Sn3, V Ngc Bi3 1 . Nha Trang Institute of Technology Research and Application, 02A Hung Vuong Str., Nha Trang City, Viet Nam 2 . Institute of Natural Products Chemistry, 18 Hoang Quoc Vit Str, Cau Giay, Ha Noi, Viet Nam 3 . Nha Trang University, 02 Nguyen Dinh Chieu St., Nha Trang City, Viet Nam * . E-mail: ndnhut@yahoo.com Many natural compounds with lipid-lowering effects have been studied including fucoidan. This work was conducted to study the blood-lipid-stable effect of low molecular weight sulfated fucoidan, derived from Sargassum polycystum fucoidan in Nhatrang brown seaweed. To study the low molecular weight sulfated fucoidan anti lipid metabolism disorders activity on guinea pig, output be found: in fed high-fat diet guinea pig model, value of lipid index increased strongly, however, if the they were fed plus low molecular weight sulfated fucoidan product (500mg/kg body weight mouse), index value of the lipid did not change compared with blank sample (not high-fat and not low molecular weight sulfated fucoidan). Key words: Activity, Anti lipid metabolism, Fucoidan, Brown Algae, Viet Nam.

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XC NH FLORFENICOL TRONG C V TM BNG PHNG PHP SC K LNG HIU NNG CAO VI U D DIOT ARRAY UV (HPLC/DAD) Trn Th Thanh Vn1, Trng Anh Tun2 1 . Vin Nghin cu v ng dng Cng ngh Nha Trang 2 . Trung tm Cht lng, An ton v sinh v Th y Thy sn vng 3 Trong bi bo ny chng ti trnh by quy trnh xc nh florfenicol trong cc mu c v tm bng phng php sc k lng hiu nng cao (HPLC/DAD) s dng ct 5m ZORBAX Eclipse Plus-C8, vi pha ng l hn hp methanol v m photsphate (t l l 25:75 v th tch) c pH=7,3 tc dng 0,8 ml/pht. Phng php chit lng-lng c s dng tch florfenicol t cc mu c v tm. Kt qu th nghim cho thy, tuyn tnh t dao ng t 25 n 500 ng/g, gii hn pht hin l 0,2 ppm,v h s thu hi florfenicol l 85,3 %. Quy trnh trn c p dng xc nh hm lng florfenicol 30 mu c v 20 mu tm thu mua t 06 siu th ti cc tnh Ph Yn, Khnh Ha, Vnh Long, ng Thp v C Mau, trong s , ch c 7 mu c cha florfenicol vi hm lng trong khong t 0,21 mg/kg to 0,84 mg. T kha: Florfenicol, HPLC/DAD, Quy trnh. DETERMINATING FLORFENICOL IN FISH AND SHRIMP BY HPLC WITH DIOT ARRAY UV DETECTION (HPLC/DAD) Tran Thi Thanh Van1,*, Truong Anh Tuan2 . Nha trang Institute of Technology research and Application, 02A Hung Vuong Str., Nha Trang City, Viet Nam 2 . The National Fisheries Quality Assurance and Veterinary Directorate - Branch 3 * . E-mail: vanvvlnt@yahoo.com.vn
1

A novel procedure for the determination of florfenicol in fish and shrimp by using reversed-phase high-performance liquid chromatography are described. Liquid chromatography was performed on a 5m ZORBAX Eclipse Plus-C8 column using methanol and 0.05 M phosphate buffer (25/75 v/v, pH 7.3) as mobile phase with the flow rate of 0.8 ml/ min and diot array detection in a wavelength 222 nm. Florfenicol was extracted from fish and shrimp by using a liquid-liquid extraction. Linearity was confirmed over the concentration range of 25500 ng/g and lowest detection limit (LOD) is 0,2 ppm. Mean extraction recovery of florfenicol from fish and shrimp was 85,3 %. Above procedure was applied to determinate the florfenicol concentration in 30 fish and 20 shrimp specimens collected from six supermarkets in Phu Yen, Khanh Hoa, Vinh Long, Dong Thap, Ben Tre and Ca Mau Provinces. As a result, florfenicol was detected in 7 out of 50 specimens with the concentration ranged from 0,21 mg/kg to 0,84 mg/kg. Key words: Florfenicol, HPLC/DAD, Procedure.

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TI U HA CC IU KIN CA PHN NG IU CH GLUCOSAMIN SUNFAT T PH PHM THY SN L Tt Thnh1 , Nguyn Tin Dng1, Chu Quang Truyn1, Nguyn Vn Sn1, Phm Minh Qun1, V nh Hong2, Phm Quc Long1 1 . Vin Ho hc cc Hp cht thin nhin 2 . B mn Ho dc - Trng i hc Bch Khoa H Ni Glucosamin l mt cht c tng hp t nhin trong c th ngi, c nhiu trong thnh phn ca sn khp. Cc mui ca glucosamin tan tt trong nc, xm nhp vo khp rt nhanh chng nn c hiu qu cao trong vic phc hi cc cu trc khp b vim. Vit Nam, hp cht glucosamin sunfat c sn xut t nguyn liu l ph phm thy sn. Hiu sut qu trnh iu ch ph thuc vo cc iu kin phn ng nh nhit , thi gian v nng cht tham gia phn ng. Bo co ny trnh by nhng kt qu t c khi tin hnh ti u ha cc iu kin nh hng trn. Cc iu kin ti u cho phn ng iu ch glucosamin sunfat t ph phm thy sn l nhit phn ng 860C; thi gian phn ng 3 gi; nng axit sunfuric 45%. iu kin trn, hiu sut iu ch glucosamin sunfat t 27,4%. T kha: Glucosamin, iu kin phn ng, Ph phm thy sn, Hiu sut. OPTIMIZING REACTIVITY CONDITION OF PREPARATION GLUCOSAMIN SUNFAT FROM FISHERIES SCRAPS Le Tat Thanh1 , Nguyen Tien Dung1 , Chu Quang Truyen1, Nguyen Van Son1, Pham Minh Quan1, Vu Dinh Hoang2, Pham Quoc Long1 1 . Institute of Natural Products Chemistry, 18 Hoang Quoc Viet St., Cau Giay, Ha Noi, Viet Nam 2 . Derpartement of Phamarceutical Chemistry, Ha Noi University of Sciences and Technology, Viet Nam * . E-mail: thanh.biotech@gmail.com Glucosamin is a synthesized compound in human body, which is considered as one of the main components of cartilage. Glucosamon salts are water dissolvable, it can penetrate quickly into arthrosis, therefore it is highly effective in restoring inflamed arthrosis. In Vietnam, glucosamine sulfate is produced from fisheries scraps. The productivity of this process depends on reaction conditions such as temperature, time, concentration of compounds involved. In this paper, we report obtained results when optimizing above conditions. The optimal conditions for the preparation of glucosamine sulfate from fisheries scraps are reaction temperature 86oC; duration 3 hours; sulfuric acid concentration 45%. At these conditions, the productivity of preparative reaction reach 27,4%. Key words: Glucosamine, Reaction condition, Fisheries scraps, Productivity.

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NGHIN CU QUY TRNH THY PHN C TP V PH LIU THY SN BNG ENZYME SN XUT SN PHM CAO M GIU AXIT AMIN NG DNG TRONG Y, DC Trung S1,*, Trn nh Toi, Nguyn nh Tuyn1, L Tt Thnh2, Hong Th Bch2, Nguyn Vn Tuyn Anh2, Phm Quc Long2 1 . Vin Ha hc; 2.Vin Ho hc cc Hp cht thin nhin Sn xut sn phm cao m giu axit amin t c bng cng ngh enzyme l gii php c ngha thit thc nhm nng cao gi tr ca ph liu thy sn. Bng cch s dng enzyme thy phn, protein nhn c s c gi tr dinh dng cao hn so vi phng php thy phn bng axit hay bng kim. Trong phn nghin cu ny, chng ti nghin cu s dng enzyme bromelanin tch t ph liu da thy phn c tp v ph liu c. Kt qu nghin cu ch ra rng, enzme bromelain hot ng ti u iu kin pH 5,5 v nhit 55- 600C v sau 48 gi thy phn. Vi quy trnh ny, t 1 kg c tp nguyn liu thu c 247g cao m giu axitamin ng dng trong i sng v y, dc. T kha: Enzyme, Thy phn, iu kin ti u, Axit amin. STUDY ON THE HYDROLYSIS PROCESS OF FISHES AND FISHERIES SCRAPS USING ENZYME TO PRODUCE HIGH PROTEIN PRODUCTS WITH AMINO ACID RICHNESS FOR MEDICAL AND PHARMACOLOGICAL APPLICATION Do Trung Sy1,*, Tran Dinh Toai, Nguyen Dinh Tuyen1 , Le Tat Thanh2, Hoang Thi Bich2, Nguyen Van Tuyen Anh2, Pham Quoc Long2 1 . Institute of Chemistry 2 . Institute of Natural Products Chemistry 18 Hoang Quoc Viet Str., Cau Giay, Ha Noi, Viet Nam * . E-mail: trungsy1977@yahoo.com Produce highly protein products with amino acid richness from fish using enzyme technology is a useful solution to improve the value of fisheries scraps. By using enzyme to hydrolyze, the protein obtained will have higher value than hydrolyze using acid or alkaline. In this paper, we use enzyme Bromelanin isolated from pineapple waste was used for hydrolyzing fishes and fisheries scraps. The results showed that the optimum conditions of enzyme bromelanin are pH 5.5 and temperature 55-60oC and after 48 hours of hydrolysis. By using this protocol, productivity from 1kg of fishes is 247g high protein products with amino acid richness for medical and pharmacological application. Key words: Enzyme, Hydrolyse, Optimum condition, Amino acid.

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TCH CHIT POLYPHENOL T RONG SARGASSUM MCCLUREI BNG PHNG PHP SC K CT V Mai Nh Hiu1, Bi Minh L1, Phm Quc Long2, Nguyn Ngc Linh1, Trn Th Thanh Vn1 1 . Vin Nghin cu v ng dng Cng ngh Nha Trang 2 . Vin Ha hc cc Hp cht thin nhin Trong bi bo ny, chng ti trnh by quy trnh tch chit polyphenol t loi rong Sargassum mcclurei bng phng php sc k ct. Trc ht, chn cht hp th tt nht chng ti tin hnh th nghim so snh cc tnh cht hp th ca nha rng EXTLITES LS 305, silicagel v PVPP (polyvinylpolypyrrolidone). Kt qu thu c cho thy nha hp th rng EXTLITES LS 305 c kh nng hp th v gii hp th ln nht trong s 03 cht hp th. Cc thng s ti u vi loi nha ny l nng TPC 5 mg/ml, th tch hp th 3 BV (th tch nha) vi tc hp th 3 ml/pht, th tch gii hp l 8 BV vi tc 3 ml/pht. Dch chit ethanol sau khi x l bng sc k ct c hm lng polyphenol tng gp 2,4 ln (t 24% n 60%) vi hiu sut thu hi l 79,1%. Kt qu nghin cu ny c th cung cp d liu khoa hc cho sn xut polyphenol vi qui m ln hn. T kha: Sagassum Mcclurei, Tch chit, Sc k ct, Thng s ti u, Polyphenol. SEPARATION OF TOTAL POLYPHENOL FROM SARGASSUM MCCLUREI BY COLUMN CHROMATOGRAPHY Vo Mai Nhu Hieu1,*, Bui Minh Ly1, Pham Quoc Long2, Nguyen Ngoc Linh1, Tran Thi Thanh Van1 1 . Nha Trang Institute of Technology research and Application , 02A Hung Vuong Str, Nha Trang city,Viet Nam 2 . Institute of Natural Products Chemistry, 18 Hoang Quoc Viet Str., Cau Giay, Ha Noi, Viet Nam * . E-mail: nhuhieu315@yahoo.com In the present study, a chromatographic method for separation of total polyphenol (TPC) from Sargassum mcclurei was investigated. The adsorption properties of three adsorbents such as macroporou resin, silicagel and PVPP (polyvinylpolypyrrolidone) were experimented in order to choose the best. Among three applied absorbents, macroporous resin EXTLITE LS 305A showed the best absorption and elution capacities. It is also reported that the optimum conditions for adsorption on EXTLITE LS 305A resin were TPC concentration at 5 mg/ml, processing volume of 3 BV, flow rate of 3 ml/min; while elution was aqueous-ethanol (20:80, V:V) of 8 BV, flow rate of 3.0 ml/min. By using this procedure, polyphenol production was increased 2,4 times (from 25 % to 60 %) with recovery yield 79.1 %. These results may provide scientific reference for larger scale -TPC production. Key words: Sargassum Mcclurei, Separation, Column chromatography, Optimum conditions, Polyphenol.

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