Bo co Ha hc Thc phm

PP xc nh tinh bt,cellulose v pectin

A. PHNG PHP XC NH TINH BT :

. S LC V TINH BT : I.1 Tinh bt ! " pol#sacca$it %&c c'u t(o t) cc *+c ,--*lucose ./ c0n* th1c t2n* 3ut 4.5H6789:n , c/ t$on* cc lai hoa 3u; , t$on* c< , th=n c=# v l c=# > I.2 Cu to : Tinh bt bao *?m hai c'u t@ l amilose v amilopectin -Amilose ! ph=n t@ l%&n* B>679-6>675 CD hEa tan t$on* n1Fc 'm v t(o nGn Cun* Cch c/ nhHt Ih0n* cao > T$on* amilose cc *+c *lucose %&c *Jn vo nhK liGn ILt 6-M , bao *?m mt s+ chuNi xLp son* son* nhau , t$on* / cc *+c *lucose c<a t)n* chuNi cun vEn* l(i hOnh xoJn +c>Ph=n t@ amilose c/ mt Pu Ih0n* Ih@ v mt Pu Ih@4Pu c/ nh/m 8H *lucosiCe: > -Amilopectin ! ph=n t@ l%&n* 9>67M-6>675 chQ hEa tan Ihi un n/n* v t(o nGn Cun* Cch c/ nhHt cao > T$on* ph=n t@ amilopectin cc *+c *lucose *Jn vHi nhau Ih0n* chQ nhK liGn ILt 6-M m cEn nhK liGn ILt 6-5 , vO vR# amilopectin c/ c'u t$Sc nhnh > Ph=n t@ amilopectin chQ c/ mt Pu Ih@ Cu# nh't > I.. T!nh "ht : Tinh bt thiGn nhiGn t) n*T c+c v cc lai c< Ihc nhau th)Fn* ch1a IhUan* V7V9W Amilose > Tinh bt n*T c+c th)Fn* t(o nGn cc Cun* Cch Xc , phY h&p cho viZc chL b[Gn cc *el thc phm > \hi s@ CXn* cc lai tinh bt ch1a hm lFn* amilose cao hFn ho]c tinh bt ^ x@ l_ aciC thO s` thu Fc cc *el $Jn hFn > I..# N$u%&n 'i(u : chQ c/ thc vRt c/ hm l%&n* tinh bt cao a;n l%&n* "%&n* tinh bt W t$on* l%&n* th0 \hoai t=# bM Bt sJn c9 "Sa mO d9 "Sa d9 H(t m(ch d9 e*0 d9 H(t Ru4*iai o(n ch[nh: 57 .hu+i c7 I..) *n$ +,n$ : Tinh bt v Cfn xu't c<a n/ cTn* c/ 1n* CXn* t$on* cc n*nh I thuRt nh% ! CZt, *i'# , sFn , I thuRt iZn Cig Tinh bt *iu amilose c/ thh CYn* chL t(o cc mn* bao in 4 bc Ijo, thu+c viGn: > Tinh bt sJn , Ihoai t=# t(o nhHt cao v [t b th/ai h/a , %&c CYn* t$on* chL T$an* 6 biLn *ia v , n1Fc s+t vHi Canh n*ha l ch't lm ]c t(m thKi h *ik cho hu#ln phY %&c ?n* nh't > Tinh bt th%Kn* Fc CYn* lm ch't t(o nhHt snh cho thc phm lUn* , l tc nh=n lm bln cc lai thc phm C(n* Ieo ho]c nhT t%Fn* , s@ CXn* nh% cc #Lu t+ ILt C[nh v lm ]c , t(o c1n* v n h?i cho nhilu thc phm >

mnH,! Tha TFn ek oinh e*u#Zt p T$%Kn* qHB\ TPH.o

T$an* 6

Bo co Ha hc Thc phm

PP xc nh tinh bt,cellulose v pectin

I.- ./ t0! 12 13i t04 : -Tinh bt c/ vai t$E Cinh C%rn* ]c biZt lHn vO t$on* 3u t$Onh tiGu h/a chSn* b th<# ph=n thnh %Kn* *lucose l ch't t(o nGn n*u?n calo ch[nh c<a thc phm cho con n*)Fi > -Tinh bt c/ nhilu 1n* CXn* 3uan t$n* vO vR# n/ c/ vai t$E 3uan t$n* t$on* cc n*nh Iinh tL v viZc s;n xu't tinh bt t) cc n*u?n n*u#Gn liZu thc vRt Fc # m(nh s nhilu n1Fc t$Gn thL *iHi > I.5 Ph67n$ 8h98 :;n <ut tinh bt "= "9" b>7" "h!nh :3u : qPu tiGn lai bU t(p ch't thu Cun* Cch ch1a tinh bt lm lJn* tinh bt tinh chL tinh bt > II.PHNG PHP XC NH TINH BT : II.1 ?h;o :9t @t :A t!nh "ht "B3 tinh bt : oi n@a c< Ihoai t=# t$Gn m]t bn ch btn* Iim lai Fc ]t t$Gn mt chRu th<# tinh an* n* mt ci $=# , $@a bt t$on* $=# vHi V77ml n1Fc c't > q2 hu#ln phY tinh bt san* c+c th<# tinh V97ml , h #Gn cho tinh bt lJn* xu+n* chJt bU n1Fc s t$Gn v $@a l(i M lPn t$Gn n1Fc c't > qh tinh bt s t$(n* thi hu#ln phY t$on* vi ml n1Fc c't qilu chL v Ih;o st h? tinh bt > II.1.1 iCu "hD : qun s0i IhUan* 677ml n1Fc c't , 2 hu#ln phY tinh bt vo n1Fc an* s0i , tiLp tXc n'u s0i t$on* 6 phSt > II.1.2 E" t!nh "B3 hF tinh bt : - qn* Vml h? tinh bt t$on* +n* thGm Vml thu+c th@ uehlin* > qun s0i cch th<# t$on* B phSt ta th'# Ih0n* c/ t$Pm U *(ch > eh% vR# h? tinh bt c/ %&c nh% t$Gn Ih0n* c/ t[nh Ih@ > Thu+c th@ uehlin* l Cun* Cch .ua8 M vHi Cun* Cch Iilm Rm ]c v Ta$t$ate Ivp eat$ium v \alium > Tc CXn* c<a Cun* Cch Iilm Rm ]c lGn .ua8 M cho h#C$oxit ?n* 4.u8,HV8: tan t$on* Cun* Cch nhK s hiZn CiZn c<a ta$t$ate > Pha chL Vml thu+c th@ uehlin* > -ThGm 9ml tinh bt , mt *it Cun* Cch ioC t$on* oCu$e Iali > ou xanh n# m't i Ihi un n/n* v mu xanh s` xu't hiZn $a Ihi h n*ui l(i > H? tinh bt cho mu xanh vHi oC s nhiZt th%Kn* > T$on* tinh bt c/ amilose tc CXn* vHi ioC s` t(o $a ph1c h&p mu xanh , Ihi / ph=n t@ ioC Fc sJp xLp s bGn t$on* vEn* xoJn c<a amilose > II.1. ThB% $i;i hF tinh bt : T$an* V a:Th<# *i;i ! un s0i cch th<# t$on* mt +n* th@ lHn V9ml h? tinh bt v 9ml H.l (m ]c > aau mNi V phSt th<# *i;i l'# mt *it n1Fc t$on* +n* th@ v nhU lGn t$Gn mt *it ioC t$on* \ ^ h swn t$Gn mt b;n th<# tinh > T$on* 3u t$Onh th<# ta c/ nhkn* mu xanh $?i t[m > Thc hiZn s th<# *i;i t$on* vEn* 69 phSt tHi V7 phSt Cun* Cch s` Ih0n* cho mu vHi ioC nka > b:Thc hiZn cc ph;n 1n* nh t[nh t$Gn n%Hc ^ th<# *i;i ! -Ph;n 1n* aelixanouu qun s0i cch th<# t$on* mt +n* th@ 6ml Cun* Cch ^ th<# *i;i t$Gn v 6ml thu+c th@ aelixanouu t$on* 9 phSt > ,, s` c/ mu cam > -Ph;n 1n* yehlin*

mnH,! Tha TFn ek oinh e*u#Zt p T$%Kn* qHB\ TPH.o

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Bo co Ha hc Thc phm

PP xc nh tinh bt,cellulose v pectin

q2 6ml thu+c th@ yehlin* vo 6ml CC *lucose > qun s0i cch th<# t$on* 9 phSt c/ t$Pm hiZn U *(ch .uV8 nhK s Ih@ .u thnh .u c<a *lucose -Ph;n 1n* phen#lh#C$azine .ch ilu chL thu+c th@ ! .=n 6* chlo$h#C$ate phen#lh#C$azine v B* .H B.88ea thGm 6ml .HB.88H Rm ]c v 9 ml n1Fc c't ,un nhj ha# un cch th<# v lJc lu h hEa tan> T$un* hEa CC ^ th<# *i;i vHi CC Iilm Rm ]c > AciC h/a l(i vHi .H B.88H,l'# 6ml CC mHi n# v thGm 6ml thu+c th@ phen#lh#C$azine > qun s0i cch th<# t$on* B7 phSt > {uan st tinh thh t$Pm hiZn btn* I[nh hihn vi4tinh thh mu vn*:> II.1.# Ph67n$ 8h98 G/nh t!nh tinh bt ! a@ CXn* thu+c th@ "u*ol ! Cun* Cch oC 7>BW t$on* \ BW thO hk% CXn* cho viZc ph=n biZt tinh bt vHi pol#sacca$it Ihc , "u*ol oC cho mu xanh Rm +i vHi tinh bt , cEn vHi pol#sacca$it Ihc Ih0n* cho mu 4t$) *l#co*en nhun* *l#co*en cho mu Ihc vHi tinh bt: > Tinh bt c/ hOnh th1c cun t$En xoJn +c nGn n*u#Gn t@ oC c/ thh sau / t%Fn* 1n* vHi hOnh th1c tinh bt t$Gn oC cun t$En > .ch tiLn hnh ! thGm V-B *it Cun* Cch "u*ol Ln 9ml Cun* Cch h Iihm t$a tinh bt , nLu c/ tinh bt ta thu %&c mu xanh Rm > II.2 Ph67n$ 8h98 <9" G/nh h2@ '6Hn$ tinh bt : >V>6 e*u#Gn tJc ! T$%Hc hLt l CYn* hNn h&p phelin* h xc nh l%&n* *luciC t2n* > Th<# ph=n mfu btn* Cun* Cch H.l , $?i xc nh %Kn* t2n* btn* ph%Fn* php Bect$anC > >V>V ei Cun* ph%Fn* php ! Hm l%&n* tinh bt t$on* mfu l hiZu s+ *ika hm l%&n* *luciC t2n* s+ v hm l%&n* %Kn* t2n* s+ xc nh theo ph%Fn* php Bect$anC v nh=n vHi hZ s+ 7,c> >V>B .ch tiLn hnh ! >V>B>6 "'# mfu v chun b mfu ! "'# mfu theo T.ne MM7c-bd > .hun b mfu theo T.ne MM6B-bd > T$an* B >V>B>V ,Xn* cX h/a ch't ! -.=n ph=n t[ch ch[nh xc Ln 7,7776*> -BOnh tam *ic Cun* t[ch V97ml v 977ml> -eSt cao su c/ *Jn sinh hn n*%&c ho]c +n* th<# tinh %Kn* I[nh 6cm,Ci 6m> -BOnh n* n%Hc ,Cun* t[ch V97ml v 977ml> -PhDu lc mM> -Pipet 9ml v V9ml> -Bu$et 67ml v V9ml> -|n* on* 67ml v 97ml> -.+c th<# tinh c/ mU Cun* t[ch 97ml,V97ml> -BOnh hSt lc Cun* t[ch 977ml,6777ml> -BFm ch=n Ih0n* ho]c vEi hSt Bume$> -BLp cch th<# ilu chQnh %&c nhiZt > -AciC H.l 6}9> -.hO axetat 67W ho]c I`m axetat V7W>

mnH,! Tha TFn ek oinh e*u#Zt p T$%Kn* qHB\ TPH.o

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Bo co Ha hc Thc phm

PP xc nh tinh bt,cellulose v pectin

-\ali oxalate b^o hEa ho]c Cinat$iphotphat b^o hEa> -ea8H V7W> -Phenolphtalein 7,6W t$on* etanol 577 > -ye 4 : sunuat 9W>HEa tan 97* ye4 : sunuat t$on* V77ml n%Hc c/ ch1a swn 67bml aciC sunuu$ic ]c4C~6,bM: Ihu'# tan ,thGm n%Hc Ln 6777ml> Ph%Fn* php n# ph;i Ih@ ye8 btn* \on8M 7,6e cho Ln Ihi c/ mu phHt h?n*> -\on8M 7,6 e -Phelin* A HEa tan 5c,V* .ua8M t$on* 977ml n%Hc c't thGm 67 ml HVa8M ]c h CD tan , thGm n%Hc c't Ln 6777ml , lJc I , lc> -Phelin* B HEa tan BM5* \on8M t$on* 977ml n%Hc c't HEa tan 677* ea8H t$on* 977ml n%Hc c't , 2 hai CC l(i vHi nhau , cho n%Hc Ln 6777ml , lJc I , lc > -H.l ]c CV7~6,66d> >V>V>B .hun b th% ! ofu ^ chun b %Fc o Ih0 btn* IhSc x( IL, t) Ih0 su# $a l%&n* mfu c=n sao cho thh t[ch \on8M 7,6e CYn* chun ntm t$on* IhUan* M-Vdml> nHi mfu ? hp v n*u#Gn liZu $au 3u; c/ Ih0 9-V7W l%&n* mfu c=n t) V7 Ln 9*> >V>V>M TiLn hnh th% ! a:c nh hm l%&n* *luciC t2n* so ! .=n 9-V7* mfu , chu#hn tEan b vo bOnh tam *ic Cun* t[ch V97ml , t$n* I c+c T$an* M c=n btn* n%Hc c't , l%&n* n%Hc cho vo bOnh IhUan* 677-697ml > ThGm 9ml aciC H.l ]c vo bOnh mfu R# nSt cao su c/ cJm +n* sinh hn n*%&c v un t$Gn bLp cch th<# s0i t$on* V *iK > "'# bOnh $a lm n*ui , t$un* hEa mfu btn* ea8H B7W , Ih@ p$otiC , lc , nh m1c nh% ph%Fn* php nh l%&n* %Kn* t2n* so > HSt 9-V9ml Cch lc,chu#hn vo bOnh tam *ic Cun* t[ch V97ml , thGm vo bOnh 97ml hNn h&p phelin* A , B v tiLp tXc un , lc , hEa tan v chun > mhi s+ ml \on8 M 7>6e ^ CYn* > Hm l%&n* *luciC t2n* s+ 4 6 :t[nh btn* W theo c0n* th1c ! a>V >677 6~ 6 mV > T$on* o ! a ! l%&n* *lucose t%Fn* 1n* 4*: n ! Cun* t[ch bOnh nh m1c 4ml: n6! thh t[ch mfu hSt h lm ph;n 1n* vHi CC phelin* 4ml: m ! l%&n* c=n mu 4*: b:c nh hm l%&n* %Kn* t2n* s+4V: ! .=n 9-V7* mu ^ chun b , chu#hn tEan b vo bOnh tam *ic V97ml , t$n* I c+c c=n btn* n%Hc c't , l%&n* n%Hc cho vo bOnh l thh t[ch , R# bOnh btn* nSt cao su c/ 7 *Jn +n* sinh hn ho]c +n* th<# tinh > qun t$Gn bLp cch th<# s b7 . t$on* 69 phSt > "'# $a h n*ui > ThGm 67ml chO axetat 67W , nLu $/t t) t) theo thnh bOnh mt CEn* m;nh chO axetat 67W m s ch2 tiLp xSc *ika hai CC Ih0n* hOnh thnh ILt t<a l s lai p$otiC ^ hEan

mnH,! Tha TFn ek oinh e*u#Zt p T$%Kn* qHB\ TPH.o

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Bo co Ha hc Thc phm

PP xc nh tinh bt,cellulose v pectin

tEan , nLu cEn ILt t<a cPn thGm CC chO axetat h lJn* > ThGm vo mu 67ml CC Iali oxalate b^o hEa , lJc I h lai chO C% , h lJn* , lc 3ua *i'# lc *'p nLp thu CC lc vo bOnh nh m1c 977ml , $@a I ILt t<a , thGm n%Hc c't Ln v(ch m1c , lJc I > HSt 97-677ml CC lc chu#hn vo bOnh tam *ic V97ml thGm 69ml H.l 6}B R# nSt cao su c/ cJm +n* th<# tinh un t$Gn bLp cch th<# s0i t$on* 69 phSt > "'# $a h n*ui , t$un* hEa CC mu btn* ea8H B7W th@ btn* *i'# chQ th > .hu#hn tEan b Cch mu vo bOnh nh m1c V97ml , thGm n%Hc c't Ln v(ch m1c , lJc I > HSt 67-V9ml CC mu vo bOnh tam *ic V97ml > .ho vo bOnh hNn h&p *?m V9ml CC Phelin* A v V9ml CC Phelin* B , lJc nhj , ]t t$Gn bLp iZn c/ l%Hi amiin* v un B phSt Ih t) lSc s0i > qh n*ui bHt v lJn* ILt t<a ?n* ox#t > "Jp hZ th+n* lc %Kn* !

T$an* 9

6 ! c+c lc c+p mM V ! bOnh hSt c/ nhnh B ! $a bFm ch=n Ih0n* ho]c vEi hSt Busne$ -"c CC 3ua phDu lc mM > .hS _ h lSc no t$Gn m]t ILt t<a cTn* c/ mt lHp CC ha# n%Hc c't > @a I ILt t<a t$Gn phDu lc vo bOnh tam *ic btn* n%Hc c't un s0i > .hu#hn phDu san* bOnh tam *ic c/ ILt t<a , hEa tan ILt t<a t$Gn phDu vo t$on* bOnh btn* 67-V7ml CC ye4 : sunuat 9W > .hun l%&n* ye4 :hOnh thnh t$on* bOnh tam *ic btn* CC \on8M7>6e cho Ln Ihi CC c/ mu h?n* sJn bln vkn* t$on* 6 phSt > mhi s+ \on8M ^ CYn* > T) ml \on8M ^ CYn* t$a b;n* Bect$anC %&c s+ m* *lucose t%Fn* 1n* chu#hn $a *am > Hm l%&n* %Kn* t2n* s+ 4V: t[nh btn* W theo c0n* th1c ! a>V6 >VB >677 V~ mV > >VV

mnH,! Tha TFn ek oinh e*u#Zt p T$%Kn* qHB\ TPH.o

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Bo co Ha hc Thc phm

PP xc nh tinh bt,cellulose v pectin

T$on* o ! a ! l%&n* *lucose t%Fn* 1n* n ! thh t[ch bOnh nh m1c mu h Ih@ p$otiC 4ml: n6 ! thh t[ch mu l'# h th<# ph=n 4ml: nV ! thh t[ch bOnh nh m1c mu ^ th<# ph=n 4ml: nB ! thh t[ch mu l'# h lm ph;n 1n* vHi Phelin* 4ml: c: Hm l%&n* tinh bt 4: t[nh btn* W theo c0n* th1c ! ~46-V:>7,c @ l_ ILt 3u; > \Lt 3u; l t$un* bOnh cn* cu; ILt 3u; V lPn xc nh son* son* > T[nh ch[nh xc Ln 7,76W > .hGnh lZch ILt 3u; *ika hai lPn son* son* Ih0n* %&c lHn hFn 7,7VW> T$an* 5 e*Eai ph%Fn* php t$Gn n*%Ki ta cEn nh l%&n* tinh bt chQ Ca vo hm l%&n* %Kn* Ih@ , su# $a hm l%&n* tinh bt , ha# CYn* ph%Fn* php chun oC > II. Ph67n$ 8h98 G/nh '6Hn$ tinh bt +I3 12o '6Hn$ G6Jn$ KhL ! >B>6 e*u#Gn tJc ! Th<# ph=n mfu ban Pu $?i nh l%&n* %Kn* t(o thnh > e*Eai cch CYn* H.l Rm ]c h th<# ph=n h? tinh bt n*)Fi ta cEn CYn* nhilu ph%Fn* php h th<# ph=n h? tinh bt> -Th<# ph=n tinh bt btn* aciC-enz#me ! Th<# ph=n tinh bt CYn* aciC xSc tc s *iai an Cch h/a v enz#me s *iai an )Fn* h/a > -Th<# ph=n tinh bt btn* enz#me-aciC ! Th<# ph=n tinh bt CYn* enz#me xSc tc s *iai an Cch h/a v aciC s *iai an %Kn* h/a > z#me c/ Ih; nin* th<# ph=n tinh bt l am#lase t(o thnh cc Cext$in c/ Ih+i l%&n* ph=n t@ Ihc nhau > \hi cho V vo cc Cext$in c/ n?n* mu Ihc nhau>Amilase %&c chia thnh B lai ! -amilase46,M-,-*lucan-*lucanoh#C$olase , . B>V>6>6:c/ t$on* n1Fc bt , h(t hEa th;o na# mPm , t$on* tX# n(n* , n'm m+i , vi Ihun > e/ ph=n *i;i liGn ILt 6,M-*lucosiCe s *ika chuNi pol#sacca$it4enCo-amilase: t(o thnh Cext$in ph=n t@ th'p > ,%Hi tc CXn* c<a n/ CC tinh bt nhanh ch/n* b m't Ih; nin* t(o mu vHi CC V v b *i;m nhHt m(nh > -amilase4. B>V>6>V:c/ nhilu s h(t , c< thc vRt > e/ xSc tc cho ph;n 1n* th<# ph=n liGn ILt 6,M-*lucosiCe t) Pu Ih0n* Ih@ t(o thnh ch< #Lu maltase v Cext$in ph=n t@ lHn > mlucoam#lase c/ nhilu s vi sinh vRt ,*an n* vRt > e/ xSc tc cho ph;n 1n* th<# ph=n liGn ILt 6,M v 6,5-*lusiCe bJt Pu t) Pu Ih0n* Ih@ c<a chuNi pol#sacca$it > a;n phm ch< #Lu l *lucose v Cext$in > >B>V nRt liZu h/a ch't ! -H(t lSa , n*0 , Ru , Ihoai t=# , Ihoai lan*> -H.l V9W> - V 7,6e > -eaVaV8B 7,6e -ea8H 7,9e -HVa8MVe -Tinh bt 6W -Thu+c th@ lu*ol >B>B .ch tiLn hnh !

mnH,! Tha TFn ek oinh e*u#Zt p T$%Kn* qHB\ TPH.o

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Bo co Ha hc Thc phm

PP xc nh tinh bt,cellulose v pectin

.=n V* n*u#Gn liZu , n*hiln nhU , t$n lu , chu#hn san* c+c , cho vo 677ml n%Hc c't , Ihu'# lu t$on* IhUan* M9-57 phSt sau / lc tinh bt btn* phDu c/ *i'# lc > T$n* l(i c+c v $@a tinh bt nhilu lPn btn* n%Hc c't h lo(i tEan b %Kn* $a IhUi tinh bt> T$an* d .hu#hn phDu lc ch1a tinh bt san* mt bOnh cPu 4V97ml: CYn* Ta th<# tinh nhU chc th<n* *i'# lc , C?n tinh bt xu+n* bOnh cPu btn* n%Hc c't 4b7-677ml: > aau / cho 6V9ml H.l V9W vo bOnh cPu , lJp hZ th+n* sinh hn , ]t bOnh cPu vo n?i cch th<# un s0i t$on* IhUan* V,9 Ln B *iK , thQnh thUan* lJc lu bOnh > aau Ihi ILt thSc thKi *ian th<# ph=n tinh bt lm n*ui bOnh t$un* hEa CC th<# ph=n btn* ea8H 67W CYn* *i'# 3u th@ sao cho CC th<# ph=n Ih0n* th)a Iilm > aau / thGm 6-V *it H.l V9W h CC th<# ph=n ^ %&c t$un* hEa c/ m0i t$%Kn* aciC #Lu > .hu#hn tEan b CC san* bOnh nh m1c V97ml , CYn* n%Hc c't h chun Ln v(ch nh m1c c<a bOnh > \hu'# lu , lc CC , thu l'# CC t$on* su+t h nh l%&n* %Kn* Ih% > ./ nhilu ph%Fn* php Ihc nhau h nh l%&n* %Kn* Ih% ! -Ph%Fn* php Be$t$anC -Ph%Fn* php so mu aau Ihi xc nh %&c Ih+i l%&n* *lucose c/ t$on* CC ph=n t[ch,ta t[nh hm l%&n* W *lucose t$on* n*u#Gn liZu theo c0n* th1c ! a>7,c>V6>677 W~ VV >g T$on* /! ! l%&n* *lucose 4m*: c/ t$on* CC ph=n t[ch n6 ! s+ ml CC th<# ph=n 4V97ml: nV ! s+ ml CC th<# ph=n em i ph=n t[ch * ! n*u#Gn liZu em i ph=n t[ch 7,c ! hZ s+ chu#hn *lucose thnh tinh bt II.# Ph67n$ 8h98 "huMn Go Io+ ! l'# V bOnh n/n cho vo bOnh 6!9 ml n%Hc c't, bOnh V! 9ml Cun* Cch th<# ph=n ^ lc, thGm vo mNi bOnh 67ml V 7>6o, lJc I t$on* V phSt sau / thGm t) t) vo bOnh 69ml ea8H 7>9o, lJc lu cho Ln Ihi m't ma4u oC> qR# nJp bOnh, h #Gn t$on* 69 phSt, sau / thGm Bml H Va8M Vo, lJc lu, ILt t<a .u V8 tan hEan tEan> .hun l%&n* V %&c tch btn* nat$ithiosuluat cho Ln Ihi Cun* Cch chu#hn san* mu vn*> ThGm mt l%&n* tinh bt 6W 4theo tQ lZ c1 6 ml tinh bt 6W t%Fn* 1n* vHi 97 ml Cun* Cch em chun : cho tHi Ihi m't mu xanh> Hm l%&n* tinh bt t[nh 4W: theo c0n* th1c sau !

X=

4V6 VV : 7>77c7>c 677 VB g

T$on* /! ! hm l%&n* tinh bt 4W: n6 ! s+ ml eaVaV8B 7>6 e chun s bOnh Iihm t$a nV !s+ ml eaVaV8B 7,6e chun s bOnh th[ n*hiZm nB ! s+ ml CC th<# ph=n em ph=n t[ch 49ml: T$an* b

mnH,! Tha TFn ek oinh e*u#Zt p T$%Kn* qHB\ TPH.o

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Bo co Ha hc Thc phm

PP xc nh tinh bt,cellulose v pectin

n ! s+ ml CC th<# ph=n ^ t$un* hEa 4V97ml: * ! s+ l%&n* n*u#Gn liZu em ph=n t[ch 4*: 7,77c !hZ s+ t[nh ILt 3u; chun V +i vHi *lucose 7,c ! hZ s+ t[nh chu#hn t) *lucose thnh tinh bt II.) Ph67n$ 8h98 thB% 8hNn bOn$ PnQ%@P 3@%'3:e ! >9>6 e*u#Gn tJc ! ,Yn* enz#me amilase th<# ph=n hon ton tinh bt thnh *lucose > .hun v nh l%&n* tinh bt Ca vo hm l%&n* *lucose t$%Hc v sau th<# ph=n > >9>V H/a ch't CXn* cX ! - .c CXn* cX h xc nh %Kn* Ih@ > - nz#m am#lase v cc h/a ch't xc nh %Kn* Ih@ > >9>B .ch tiLn hnh ! .=n 6-V* mfu 4on:, n*hiln nhu#Dn t$on* c+i s1 , chu#hn vo beche$ v thGm vo B7ml n%Hc c't> \hu'# un s0i h h? h/a tinh bt > "m n*ui tHi M7 7. v thGm vo Vml Cun* Cch enz#me am#lase h th<# ph=n hon ton tinh bt t$on* V7 phSt> \ihm t$a ^ th<# ph=n hon ton btn* mt *it thu+c th@ "u*ol> aau / tiLn hnh *i+n* nh% +i vHi mfu xc nh %Kn* Ih@ btn* ph%Fn* php ue$$#c#anu$e> >9>M T[nh ILt 3u; ! Hm l%&n* W tinh bt t$on* mfu n*u#Gn liZu ! TB ~ 4tb p I: 7>c T$on* / ! tb ! hm l%&n* W %Kn* Ih@ sau th<# ph=n I ! hm l%&n* W %Kn* Ih@ t$%Hc th<# ph=n 7>c ! hZ s+ chu#hn 2i t) *lucose san* tinh bt BRPHNG PHP XC NH CSTTUTVWS : I.S LC V CELLULOSE : I.1 CP''u'o:P l pol#sacca$it ch< #Lu c<a thnh tL bo thc vRt chiLm IhUan* BV-MdW vRt ch't Ih0 c<a th1c in th0> I.2 Cu to : .ellulose l chuNi cacbonh#C$at Fn *i;n , ph=n t@ m(ch thn* %&c c'u t(o bsi -, *lucose btn* liGn ILt 6,M-*lucosiCe> oNi ph=n t@ c/ thh lGn tHi hn* v(n Fn v> T$on* t nhiGn cellulose t?n t(i C%Hi C(n* tinh thh , cellulose bao *?m nhilu chuNi thn* liGn ILt vHi nhau thnh b/ Ci nhK m(ch n+i h#C$o*en t(o thnh cc s&i cellulose bln vkn* 4mic$ouib$il: %&c bao bc bsi thnh phPn Ihc c<a vch tL bo, cc mic$ouib$il tRp h&p l(i thnh cc macc$ouib$il> T$an* c I. T!nh "ht ! .ellulose l ch't xF Ih0n* hEa tan c/ ]c t[nh thm th'u t$on* $ut, t$%Fn* lGn t(o ilu IiZn cho ch't b^ th;i $a n*Eai> BOnh th%Kn* Ih bln vkn*> ou+n biLn thnh ph=n t@ %Kn* ph;i un n/ s nhiZt cao vHi ax[t ho]c Iilm ]c , tu# nhiGn enz#me cenllulose l(i ph=n h<# n/ mt cch CD Cn*>

mnH,! Tha TFn ek oinh e*u#Zt p T$%Kn* qHB\ TPH.o

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Bo co Ha hc Thc phm

PP xc nh tinh bt,cellulose v pectin

I.#N$u%&n 'i(u ! .ellulose c/ nhilu t$on* vU hoa 3u; v $au T$on* b0n* n/ t$Gn c7W, *N hFn 97W, vU c phG 5B,VW I.) *n$ +,n$ : -,fn xu't c/ cenllulose c/ _ n*ha $'t 3uan t$n* t$on* nhilu n*nh c0n* n*hiZp ! s&i nh=n t(o 4s&i cellulose axetat:, Ca nh=n t(o , I thuRt ch't n2 , ch't Co , s;n xu't *i'# g I.- ./ t0! 12 13i t04 ! .ellulose Ih0n* c/ _ n*ha Cinh C%rn* c<a n*%Ki vO Ih0n* tiGu h/a %&c t$on* +n* tiGu h/a n/ *iSp cho viZc tiGu h/a %&c CD Cn*> qn* vRt nhai l(i c/ thh tiGu h/a CD Cn* cellulose vO t$on* $ut c<a chSn* c/ ch1a cc vi Ihun c/ Ih; nin* tiLt $a enz#me cellulose l enz#me thT# ph=n cellulose > ot *N t$on* C( C# c<a chSn* c/ ch1a nhkn* n* vRt n*u#Gn sinh 4tiGn mao t$Yn* : < Ih; nin* ph=n th<# cellulose thnh %Kn* *lucose cun* c'p nin* l%&n* cho n/> II.PHNG PHP XC NH CELLULOSE : II.1 ?h;o :9t @t :A t!nh "ht "B3 "P''u'o:P : >6>6 Th<# *i;i mt phPn ! a: e*u#Gn tJc ! ehK s th<# *i;i mt phPn c<a cellulose, n*%Ki ta c/ %&c nhkn* oli*osaccha$iCe> ehkn* ph=n t@ oli*osaccha$iCen# cho mu xanh vHi ioC, mt t[nh ch't m cellulose Ih0n* c/ %&c vO C^# C=# v thn* c<a n/ 3u'n t$En mt cch ch]t ch` v Ih0n* cho ioC len li vo > b: Th%c hnh ! ehSn* mt miLn* nhU b0n* th'm n%Hc 4cellulose Ih tinh ch't: vo Cun* Cch ioC t$on* \ > aau vi phSt l'# miLn* b0n* $a v vp Ih0 , an bU miLn* b0n* vo d9W t$on* vi phSt nhkn* s&i b0n* biLn thnh mu xanh Rm > >6>V Th<# *i;i ton phPn ! q2 vo c+c th<# tinh cr 97ml, 9mlHVa8MRm ]c > BU vo c+c th<# tinh mt [t b0n* th'm n%Hc 4cotton h#C$ophile: 3ua# lu cellulose cho tHi Ihi tan lu t$on* ax[t q2 Cun* Cch n# vo mt +n* th@ lHn n* swn 69ml n%Hc l(nh , un s0i cch th<# t$on* B7 phSt > T$an* 67

B=# *iF t$Gn ch't lUn* ^ th<# *i;i ta thc hiZn nhkn* ph;n 1n* aelixanouu , yehlin*, phen#lh#C$azine nh% t$Gn v ta cTn* thu %&c ILt 3u; t%Fn* t c<a tinh bt> e*Eai ph%Fn* php CYn* aciC h th<# ph=n cellulose n*%Ki ta cEn CYn* enz#me cellulose h th<# ph=n cellulose> .hSn* lm viZc btn* cch cJt cc h&p ch't ph1c h&p 4h&p ch't nln: thnh nhkn* h&p ch't Fn CD Cn* cho vi Ihun s< CXn* nh% l ch't Cinh C%rn* c<a chSn* > II.2 Ph67n$ 8h98 <9" G/nh h2@ '6Hn$ "P''u'o:P thXYGF h8Z : >V>6 ei Cun* ph%Fn* php ! Th<# ph=n cc ch't hk% cF Ih0n* ph;i cellulose btn* aciC $?i btn* Iilm v c?n , phPn cEn l(i l cellulose th0 %&c nh ph=n btn* ph%Fn* php Ih+i l%&n*>

mnH,! Tha TFn ek oinh e*u#Zt p T$%Kn* qHB\ TPH.o

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PP xc nh tinh bt,cellulose v pectin

>V>V "'# mfu ,chun b mfu theo nhkn* 3u# nh t$on* T.ne M9cM-bb >V>B ,Xn* cX ,h/a ch't ! -.=n ph=n t[ch ch[nh xc Ln 7,776* -T< s'# ilu chQnh %&c nhiZt -"E nun* ilu chQnh %&c nhiZt > -BOnh cPu # t$En 977ml -|n* sinh hn h?i l%% -.+c nun* -HVa8M6,9W -tanol c57 -te et#lic >V>M TiLn hnh th@! .=n 67-V7* mfu ^ chun b t$Gn c=n ph=n t[ch lo(i bF mr t$on* mfu,nLu mfu nhilu mr CYn* ph%Fn* php chiLt solac,nLu [t mr CYn* ete Ihan,Ihu'# I lc *(n vi ba lPn> .hu#hn mfu ^ lai mr vo bOnh cPu c't, cho vo bOnh V77ml H Va8M6,9W ^ un s0i swn, lJp +n* sinh hn n*%&c vo bOnh cPu,un t$Gn bLp cch th<# s0i t$on* B7 phSt lJc lu0n h t$nh mfu b cacbon h/a, lc mfu ^ th<# ph=n aciC 4CYn* m# li t=m , m# hSt ch=n Ih0n* ho]c phDu lc:> @a phPn Ih0n* hEa tan nhilu lPn btn* n%Hc c't un s0i cho Ln Ihi n%Hc $@a Ih0n* cEn aciC, th@ btn* *i'# o PH> .hu#hn ton b b^ Ih0n* tan ^ th<# ph=n aciC v $@a s(ch vo bOnh cPu c't, t$n* I *i'# lc ho]c +n* ch1a b^ btn* n%Hc c't un s0i,l%&n* n%Hc t$n* t2n* cn* l 677ml> ThGm 677ml CC ea8H V-9W ^ un s0i vo bOnh ,lJp +n* sinh hn,un s0i v *ik t$Gn bLp cch th<# s0i B7 phSt > "c l'# xF b^ Ih0n* hEa tan , $@a s(ch btn* n%Hc s0i Ln t$un* t[nh th@ btn* *i'# o PH > qh $o n%Hc btn* c?n c57 hai lPn , mNi lPn Iho;n* 9ml> T$an* 66 .hu#hn ton b xF b^ vo nt chvn nun* ^ s'# Ih0 c=n bO> a'# chvn c/ xF b^ t$on* t< s'# s nhiZt 6797. t$on* 6 *iK , h n*u0i c=n> a'# l(i cho Ln Ih+i l%&n* Ih0n* 2i> .hu#hn chvn nun* vo lE nun* Ln t$o t$Jn* lm n*ui t$on* bOnh hSt m , c=n> eun* Ln Ih+i l%&n* Ih0n* 2i> >V>9 T[nh ILt 3ua ! Hm l%&n* cellulose th04: %&c t[nh btn* W theo c0n* th1c ! 4m mV :>677 ~ 6 m T$on* /! o6! Ih+i l%&n* chvn nun* v xF b^ sau s'# 4*: oV! Ih+i l%&n* chvn nun* v t$o sau Ihi nun* 4*: o! l%&n* c=n mfu 4*: Hm l%&n* cellulose th0 cEn %&c theo Ih+i l%&n* thnh phPn th0 nh% sau ! 4m6 mV :>677 ~ m4677 H : T$on* o ! H ! m c<a thc phm t[nh btn* W \Lt 3u; l t$un* bOnh cn* c<a ILt 3u; hai lPn xc nh son* son* t[nh ch[nh xc 7,76W, chGnh lZch ILt 3u; hai lPn Ih0n* %&c 3u 7,7VW>

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PP xc nh tinh bt,cellulose v pectin

III. PHNG PHP XC NH CH[T X : III.1 PHNG PHP XC NH CHT X CH!: III.1.1 T% @\u 13 "huMn b/ @\u ! -"'# mfu theo T.ne MM7c-bd -.hun b mfu theo T.ne MM6B-bd III.1.2 Ph67n$ 8h98 <9" G/nh : >6>V>6 H/a ch't v thu+c th@ ! -HVa8M CC 6,V9W -ea8H CC 6,V9W -H.l CC 6W -.?n -Ciet#l ete -mi'# 3u >6>V>V TiLn hnh th@ ! .=n IhUan* V,9* ch vHi ch[nh xc 7,776*, cho mfu vo c+c Cun* t[ch 577ml, thGm vo V77ml CC HVa8M ]c vo t< hSt v un Ln s0i, thQnh thUan* lJc h ch Ih0n* bm vo thnh c+c > qun s0i t$on* B7 phSt> eLu cPn c/ thh thGm mt [t ch't Ih@ bt> T$on* 3u t$Onh un,b2 sun* n%Hc vo phPn n%Hc ba# hFi> "'# c+c $a, thGm vo 97ml n%Hc l(nh v lc nhanh 3ua v;i lc, $@a c]n btn* n%Hc s0i cho Ln T$an* 6V Ihi Cch $@a Ih0n* cEn aciC 4th@ btn* *i'# 3u: > .ho c]n vo c+c ,thGm V77ml CC ea8H ,]t c+c lGn bLp v un s0i t$on* B7 phSt>ThQnh tho;n* lJc h c]n Ih0n* bm vo thnh c+c>PhPn n%Hc ba# hFi %&c b2 sun* btn* n%Hc s(ch "c v cho c]n vo c+c un,$@a c]n C[nh l(i s c+c h l'# hLt c]n>@a c]n btn* n%Hc n/n* sau / btn* CC H.l v cu+i cYn* btn* n%Hc n/n* cho Ln Ihi Ih0n* cEn aciC4th@ btn* *i'# 3u: .u+i cYn* $@a c]n btn* c?n $?i btn* Ciet#l ete>,a vo m# h lo(i bU cc vZt Cun* m0i> .ho c]n vo t< s'#,s'# s nhGt 677 V7.,lm n*ui $?i c=n,l]p l(i cc thao tc n# cho Ln Ihi chGnh lZch Ih+i l%&n* *ika hai lPn c=n liGn tiLp Ih0n* v%&t 3u 7,776*, *hi ILt 3u; cu+i cYn* l'# ch[nh xc Ln 7,776* .ho c]n sau Ihi s'# vo lE nun* c/ nhiZt 9V9 V77. nun* Ln Ih+i l%&n* Ih0n* 2i ,lm n*ui $?i em c=n >6>V>B T[nh ton ILt 3ua ! Hm l%&n* xF 4: t[nh btn* W ch't Ih0, xc nh theo c0n* th1c ! 4m6 mV :>677>677 ~ m7 46 ": T$on* /! m 6 !Ih+i l%&n* c]n sau s'# 4*: m V !Ih+i l%&n* c]n sau Ihi nun* 4*: m 7 !Ih+i l%&n* mfu 4*: x! m c<a che 4W:

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III.# .PHNG PHP XC NH H$% LNH X TH& T'ONG TH(C )N CH)N NU&I: III.2.1 T% @\u thL theo nhkn* tiGu chun ^ 3u# nh t$on* T.ne MBVc-6ccB III.2.2 Ni +un$ 8h67n$ 8h98 ! ,Yn* Cun* Cch aciC v Iilm vHi n?n* nh't nh th<# ph=n v tch IhUi mfu th@ cc ch't bt, %Kn* , p$otiC, CPu mr, mt phPn hemicellulose v li*nin cEn l(i *i l xF th0> III.2. ],n$ ", h=3 "ht: >V>B>6 ,Xn* cX! -.=n ph=n t[ch vHi ch[nh xc 7,777V* -"E nun* c/ ilu chQnh nhiZt 997 V97. -t< s'# c/ ilu chQnh nhiZt V7. -o# hSt ch=n Ih0n* -BLp iZn -BOnh hSt m T$an* 6B -BOnh n*%n* l(nh -.+c 0i Cun* t[ch M97-977ml -PhDu lc ,F$un*Ci -BOnh BunCen Cun* t[ch V-Bl -.hvn lc btn* s1 c/ nJp -BOnh nh m1c Cun* t[ch 6777ml -B0n* amian -qTa th<# tinh s Pu c/ bt cao su >V>B>V H/a ch't! -HVa8M CC 6,V9W, hEa tan dml HVa8M c/ t t$n* 6,bM*}ml vo n%Hc c't, sau Ihi h n*ui thGm n%Hc c't Ln 6l -\8H CC V,9W, hEa V9* \8H t$on* n%Hc c't Ln 6l -.?n etanol c5W -te pet$ol III.2.# TiDn h2nh thL: >V>M>6 .=n t) 6,9 Ln V* vHi ch[nh xc 7,776* mfu th1c in s C(n* Ih0 Ih0n* Ih[ ^ %&c n*hiln nhU v cho vo c+c 0i Cun* [ch M97-977ml c/ v(ch s m1c V77ml> eLu th1c in c/ hm l%&n* ch't bvo lHn hFn 67W thO cPn ph;i chiLt ch't bvo t$%Hc Ihi xc nh xF tho> >V>M>V /t vo c+c V77ml HVa8M 6,V9W ^ %&c un n/n* d7-b77.,CYn* Ta th<# tinh Ihu'# lu R# c+c btn* bOnh n*%n* l(nh > q]t c+c t$Gn bLp v un Ln s0i thRt nhanh 4t$on* Iho;n* V phSt: tiLp tXc un s0i nhj t$on* B7 6 phSt> T$on* 3u t$Onh un CYn* Ta th<# tinh Ihu'# lu h nhkn* h(t th1c in Ih0n* bm vo thnh c+c> oc n%Hc t$on* c+c lu0n *ik s v(ch V77ml, nLu c(n ph;i thGm n%Hc c't n/n*> >V>M>B aau Ihi th<# ph=n btn* aciC, h lJn* v lc Cch cEn an* n/n* 3ua phDu ,F$an*Ci vHi *i'# lc, nLu hm l%&n* xF th0 lHn hFn BW thO c/ thh CYn* phDu th<# tinh c/ bt v;i nilon vHi lN th%a Ih0n* 3u 7,6mm h hSt lc btn* t$an* b hSt ch=n Ih0n* vo bOnh BunCen>

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Bo co Ha hc Thc phm

PP xc nh tinh bt,cellulose v pectin

,Yn* bOnh tia n* n%Hc c't n/n* $@a c]n bm s v;i lc, *i'# lc , thnh c+c xu+n* c+c, thGm n%Hc c't n/n* vo c+c cho Ln v(ch m1c , h lJn* v tiLp tXc hSt $@a nhkn* ch't Ih0n* hEa tan cho Ln Ihi n%Hc lc (t t$un* t[nh Ihi th@ btn* *i'# 3u> >V>M>M .ho vo c+c 67ml CC \8H V,9W thGm n%Hc c't n/n* Ln v(ch V77ml lJp bOnh n*%n* l(nh > q]t c+c lGn bLp v un s0i lu CC t$on* B7 6 phSt Ih t) Ihi bJt Pu s0i> HSt lc v $@a c]n xF t$on* c+c nh% s t$Gn> >V>M>9 .hu#hn ton b CC v c]n t) c+c san* chvn lc s1 s # c/ l/t Iho;n* V* amian ^ %&c s'# Ih0 v xc nh Ih+i l%&n* Amian t$%Hc Ihi s@ CXn* ph;i 3ua x@ l_ un s0i t$on* B7 phSt t$on* CC HVa8M T$an* 6M 6,V9W $@a s(ch un s0i t$on* B7 phSt t$on* CC \8H 6,V9W, $@a s(ch s'# Ih0 v nun* s nhiZt 9977. t$on* thKi *ian V *iK> ,Yn* n%Hc c't n/n* $@a s(ch mfu t$on* chvn lc sau '# lPn l%&t CYn* 69ml etanola c5W v 69ml ete h $@a mfu> >V>M>5 a'# chvn lc cYn* vHi mfu s nhiZt 679 7. h n*ui t$on* bOnh hSt m v c=n xc nh Ih+i l%&n*> "]p l(i 3u t$Onh s'# v c=n cho Ln Ihi chGnh lZch *ika V lPn c=n liGn tiLp Ih0n* 3u 6m*> eun* chvn lc cYn* vHi mfu s nhiZt 9977. V97. t$on* thKi *ian V *iK h n*ui t$on* bOnh hSt m v c=n xc nh Ih+i l%&n*> III.2.) C9"h t!nh KDt ^u;! Hm l%&n* xF th0 4: t$on* mfu th1c in t[nh btn* phPn t$im t[nh theo c0n* th1c sau! m>677 ~ m6 T$on* /! m! Ih+i l%&n* xF th0 t$on* mfu t[nh btn* *am, btn* Ih+i l%&n* chvn lc vHi mfu t$%Hc Ihi nun* t$) Ih+i l%&n* chvn lc vHi mfu sau Ihi nun*> m6! \h+i l%&n* mfu th@ t[nh btn* *am> \Lt 3u; cu+i l t$un* bOnh c<a ILt 3<a V lPn ph=n t[ch son* son*, nLu nh% sai Ihc *ika hai lPn ph=n t[ch Ih0n* 3u! 7>M 4 tu#Zt +i: Ihi xF th0 t$on* mfu th'p hFn 67W> MW 4 t%Fn* /i: nLu hm l%&n* xF th0 t$on* mfu lHn hFn 67W> I..PHNG PHP NH T_NG CSTTUTVWS: I..1 N$u%&n t`" : .ellulose l ch't xF b^ cEn l(i sau Ihi cc *luciC Ihc nh% tinh bt , li*nin v cc sJc t+ , cc t(p ch't b th<# ph=n bsi aciC v Iilm > aauI hi lc, $@a s(ch xF b^ n# , s'# Ih0 Ln Ih+i l%&n* Ih0n* 2i v nun* thnh t$o , t$) hm l%&n* t$o , t[nh %&c l%&n* cellulose t$on* mfu> I..2 ],n$ ", a h=3 "ht : - T< s'#, lE nun*, c+i ch# s1, chvn nun* - BOnh cPu 697 c/ *Jn +n* sinh hn Ih[ , phDu , *i'# lc Ih0n* t$o , beche$ - H.l ]c , \8H 67W, .HB.88H VW - Thu+c th@ "u*ol

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6B

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Bo co Ha hc Thc phm

PP xc nh tinh bt,cellulose v pectin

I.. C9"h tiDn h2nh : .=n V9* n*u#Gn liZu ^ %&c n*hiln mn, cho vo mt bOnh cPu cao c2 ^ ch1a 97ml n%Hc c't , thGm vo 67ml H.l Rm ]c> q]t bOnh cPu lGn bLp iZn t$on* t< h+t, *Jn +n* sinh hn Ih[ v un s0i that I cho Ln Ihi tinh bt b th<# ph=n hon ton , th@ ihm ILt thSc th<# ph=n btn* cch T$an* 69 ch'm mt *it Cun* Cch th<# ph=n lGn lam I[nh vo *it thu+c th@ "u*ol Ln m't mu xanh> "'# bOnh cPu $a IhUi bLp, lc 3ua *i'# lc Ih0n* t$o, l'# b^> @a b^ nhilu lPn btn* n%Hc c't > ,Yn* bOnh tia n%Hc chu#hn hLt b^ vo l(i bOnh cPu ^ CYn* t$Gn ThGm vo bOnh cPu 67ml \8H 67W> q]t bOnh cPu lGn bLp, un s0i V7-B7 phSt nka> "c l'# b^ t$Gn hai *i'# lc Ih0n* t$o c/ t$n* l%&n* btn*n* nhau> @a b^ 9 lPn btn* n%Hc c't, mNi lPn V7ml> aau / $@a ba lPn btn* Cun* Cch .H B.88H VW $?i btn* $%&u et#lic n*u#Gn ch't 4c?n tu#Zt +i:> aau Ihi $@a b^ , b^ c/ mu t$Jn* t$on* l %&c> q]t c; hai *i'# lc v b^ vo a Pet$i $?i %a vo t< s'# 679 7. t$on* V *iK, l'# $a h n*ui t$on* bOnh hSt m v c=n> q%a c; hai *i'# lc v b^ vo chvn nun* 4^ %&c nun* t$on* lE nun* s M77-9777. h n*ui v c=n t$Gn c=n ph=n t[ch:, tiLn hnh nun* b^ t$on* lE nun* s nhiZt M77-977 7. t$on* B *iK Ln Ih+i l%&n* Ih0n* 2i "'# $a h n*ui t$on* bOnh hSt m v c=n chvn nun* ch1a t$o sau Ihi nun* I..# T!nh KDt ^u;: "%&n* cellulose c/ t$on* mfu %&c t[nh theo c0n* th1c! 4m 4mV mB : 677 W~ 6 m T$on* /! m6 ! Ih+i l%&n* b^ sau Ihi s'# 4*: mV ! Ih+i l%&n* chvn nun* v t$o sau Ihi nun* 4*: mB ! Ih+i l%&n* chvn nun* 4*: m! Ih+i l%&n* mfu 4*: ]RPHNG PHP XC NH PSCTIN: I. S LC V PECTIN: I.1 PP"tin l pol#sacca$it c/ nhilu s 3u; ,c< ho]c th=n c=# I.2 T!nh "ht ! T$on* thc vRt pectin t?n t(i C%Hi hai C(n*! -P$otopectin Ih0n* tan ,t?n t(i ch< #Lu s thnh tL bo c/ l` C%Hi C(n* ILt h&p vHi pol#sacca$it a$aban> -Pectin hEa tan t?n t(i ch< #Lu s Cch tL bo > ,%Hi tc CXn* c<a aciC,c<a enz#me p$otopectinaza ho]c Ihi un s0i p$otopectin chu#hn san* C(n* pectin hEa tan q]c t[nh 3uan t$n* c<a pectin l Ihi c/ m]t aciC v %Kn* n/ c/ Ih; nin* t(o thnh ch't *el vO vR# n/ %&c 1n* CXn* ph2 biLn t$on* I n*hZ s;n xu't m1t Ijo>qh

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PP xc nh tinh bt,cellulose v pectin

t(o thnh ch't *el pectin c/ thh thGm %Kn* sacca$oza tHi tQ lZ 59-d7W4b^o hEa %Kn*: v t(o m0i t$%Kn* c/ PH Iho;n* B,6-B,9 nhK cc aciC hk% cF nh% aciC cit$ic Pectin l'# t) cc n*u?n Ihc nhau s` Ihc nhau vl Ih; nin* t(o *el v Ihc nhau [t nhilu vl s+ cc nh/m thL .HB8-4metox#: t$on* ph=n t@ Pectin hEa tan l pol#sacca$it c'u t(o bsi cc *+c aciC *alactu$onic t$on* / mt s+ *+c aciC c/ ch1a nh/m thL metox#> Ph=n t@ l%&n* c<a cc lo(i pectin l'# t) cc n*u?n 3u; Ihc nhau tha# 2i t$on* *iHi h(n $n* $^i>n[ CX t) n*u?n to , mRn ^ thu %Fc pectin c/ ph=n t@ l%&n* t) V9777B9777,t$on* Ihi / pectin l'# t) cam l(i c/ ph=n t@ l%&n* (t tHi 97777> TGn *i pectin CYn* h chQ cc chuNi pol#*alactu$onic met#l h/a 677W> TGn *i aciC pectinic h chQ ch't %&c met#l h/a th'p hFn 677W> TGn *i aciC petic h chQ aciC pol#*alactu$onic hon ton Ih0n* ch1a nh/m metox#> T$on* thc tiDn thO tGn pectin CYn* h chQ c; aciC pectinic v pectin> TQ lZ met#l h/a %&c bihu hiZn btn* chQ s+ metox# >a met#l h/a hon ton t%Fn* 1n* vHi chQ s+ metox# btn* 65,BW cEn cc pectin tch $a t) thc vRt th%Kn* c/ chQ s+ metox# t) 67 Ln 6VW .hilu Ci c<a chuNi aciC pol#*alactu$onic c/ thh biLn 2i t) vi Fn v tHi hn* t$im Fn v aciC *alactu$onic> ot s+ nh/m h#C$ox#l c/ thh b axet#l h/a >T$on* thc vRt cc pectin th%Kn* liGn ILt vHi cellulose s vch tL bo C%Hi C(n* ph1c h&p ch%a biLt $ > I. .3i t04: .c ch't pectin *ik vai t$E 3uan t$n* t$on* 3u t$Onh ch[n c<a 3u; > \hi 3u; an* pht t$ihn p$otopectin ph=n tn s thnh tL bo v chiLm tQ lZ Ih cao , tHi lSc 3u; bJt Pu ch[n p$otopectin biLn CPn san* C(n* pectin hEa tan C%Hi tc CXn* c<a aciC hk% cF v enz#me p$otopectinase t$on* 3u;> T$on* Ihi b;o 3u;n cTn* nhRn th'# s *i;m CPn p$otopectin v tin* CPn pectin hEa tan> \h; nin* t(o *el %&c 1n* CXn* $n* $^i t$on* I thuRt thc phm \h; nin* t(o *el c<a pectin phX thuc vo hai #Lu t+! chilu Ci c<a chuNi pectin v m1c met#l h/a > .hilu Ci ph=n t@ 3u#Lt nh c1n* c<a *el> eLu ph=n t@ pectin c/ chilu Ci 3u th'p thO n/ s` Ih0n* t(o %Fc *el m]c CY s@ CXn* lilu l%&n* cao> TY# thuc vo chQ s+ metox# cao4dW: ho]c th'p4B-9W: s ph=n t@ pectin m cc Iihu ILt h&p *ika chSn* s` Ihc nhau t$on* viZc t(o *el! \hi chQ s+ metox# cao ! liGn ILt *ika cc ph=n t@ pectin ch< #Lu nhK cPu h#C$o *ika cc nh/m h#C$ox#l> \ihu liGn ILt n# Ih0n* bln Co / cc *el t(o thnh s` mlm Co Co t[nh Ci n* c<a cc ph=n t@ t$on* Ih+i *el, lo(i *el n# Ihc biZt vHi *el th(ch ho]c *elatin> T$an* 6d -\hi chQ s+ metox# th'p! c/ thh t(o *el Ihi CYn* mt l%&n* canxi C%Hi 7,6W miDn l chilu Ci ph=n t@ pectin ph;i (t m1c nh't nh > \hi / *el %&c hOnh thnh n*a# c; Ihi Ih0n* thGm %Kn* v aciC > n*ha thc tL c<a cc pectin c/ chQ s+ metox# th'p l n/ cho phvp chL t(o %&c m1t 3u; 0n* ,ska 0n* m Ih0n* cPn thGm %Kn* ho]c t(o %&c

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tht 0n* c/ c1n* bln n*a# c; s Ih[ hRu nhiZt Hi4Ihc vHi 0n* *elatin:>mel pectin c/ chQ s+ metox# th'p th%Kn* c/ t[nh ch't n h?i *i+n* nh% *el a*a-a*a> II. PHNG PHP XC NH PECTIN: II.1 iCu "hD 8P"tin! e*u#Gn liZu 3uan t$n* nh't %&c CYn* h chL t(o pectin l vU cam 3u_t v b^ to > .c phX phm n# %&c s'# Ih0 b;o 3u;n h s@ CXn* su+t nim > T$on* cc lai 3u; h cam 3u_t thO chanh v b%si %&c %a th[ch hFn cam > "%&n* pectin s vU cam 3u_t chiLm t) V7-97Wt$n* l%&n* Ih0 ,cEn s b^ to t) 67-V7W> T$on* thc tL n*%Ki ta bihu th Ih; nin* t(o *el c<a cc lai pectin btn* chQ s+ ha# bRc t(o *el> nHi 6* b^ to Ih0 , bRc t(o *el 1n* vHi V9-B9, cEn cYn* l%&n* vHi vU cam 3S#t Iho thO bRc t(o *el (t [t $a l 5 lPn cao hFn bRc t(o *el c<a b^ to Ih0 > nU cam 3S#t %&c t$[ch li tinh CPu %&c $@a s(ch ,cJt nhU $?i $@a n%Hc 'm 497-57 7.: h lo(i bU cc *lucozit cEn s/t l(i > aau / %a nhiZt lGn Ln c9-cb 7. h lm m't ho(t t[nh enz#me ph=n *i;i pectin> e%Hc CYn* t$o* s;n xu't pectin ph;i ch1a [t Ihon* , nh't l canxi v ma*ie ha# t+t hFn c; l v0 Ihon* > .Pn un n/n* v)a ph;i v nhanh h Ih0n* ph=n *i;i pectin > aau / l *iai o(n chiLt $St pectin btn* cch un n/n* t$on* n%Hc ch1a aciC > Th0n* th%En* n*%Ki ta CYn* l%&n* n%Hc *'p ba lPn l%&n* vU Ih0 , PH 6,B-6,M, nhiZt c7-6777. v thKi *ian un l 6 *iK > HNn h&p thu %&c s` %&c vp btn* m# vp th<# lc, sau / CC pectin %&c lm l(nh v lJn* *(n > ot [t t(p ch't nh% tinh bt v p$otein lfn vHi pectin s` %&c lo(i bU nhK cc enz#me ph=n *i;i p$otein> niZc x@ l_ n# %&c thc hiZn s PH M,9 v s nhiZt M7-97 7.> \hi ^ lo(i hLt tinh bt 4Iihm t$a btn* ioC: ilu chQnh PH CC tHi B btn* cch thGm aciC cit$ic $?i %a nhiZt lGn b7 7. h lm m't ho(t t[nh c<a enz#me>,, c/ thh %&c lm m't mu nhK anh#C$ite suluu$F, $?i cho lc vp thu CC pectin t$on* su+t> .0 ]c CC $?i ILt t<a pectin btn* etanol 4ho]c izop$opanol: ho]c btn* nh0m sunuat> II.2 Ph67n$ 8h98 G/nh '6Hn$ 8P"tin: >V>6 ei Cun* ph%Fn* php! Pectin A>pectic Pectat .a >V>V Thc hiZn ! ,Yn* tc nh=n aciC ho]c enz#me p$otopectinase ho]c un s0i h chu#hn ton b p$otopectin san* C(n* pectin hEa tan> .ho pectin hEa tan tc CXn* vHi ch't Iilm lo^n* ho]c enz#me pectase s` *i;i T$an* 6b ph/n* nh/m metox# C%Hi C(n* $%&u met#lic v pol#sacca$it cEn l(i Ihi / l aciC pectic t Co , n*ha l aciC pol#*alactu$onic> T) C(n* aciC pectic t(o nGn C(n* mu+i canxi pectat,ch't n# CD Cn* chu#hn thnh C(n* ILt t<a > T) l%&n* ILt t<a c/ thh CD Cn* su# $a l%&n* pectin>

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T$an* 6c

MC LC T$an* * PHNG PHP XC NH TINH BT

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PP xc nh tinh bt,cellulose v pectin

1 2 c 1d 12 12 1 11l

I. W T_C .b TINH BT II. PHNG PHP XC NH TINH BT B. PHNG PHP XC NH CELLULOSE I. W T_C .b CSTTUTVWS II. PHNG PHP XC NH CSTTUTVWS III. PHNG PHP XC NH CH[T X III.1 PHNG PHP XC NH CH[T X e CHf III.2 PHNG PHP XC NH Hgh T_NG X THi TjVNG TH*C kN CHkN NUiI C. PHNG PHP XC NH PECTIN I. W T_C .b PSCTIN II. PHNG PHP XC NH PSCTIN

T2i 'i(u th3@ Kh;o 6> e*u#Dn q1c "%Fn*, .ao .%Kn*> T+, -g+./m 01-g -g+/ 2.-+ +30 4567 189 T+, -g+./m +:a 2.-+ +30, eB q(i hc Bch \hoa TPH.o> V> T.;< 0+<=- V./5 Nam, T.ne M9c7-bb B> T.;< 0+<=- V./5 Nam, T.ne M9cM-bb M> T.;< 0+<=- V./5 Nam, T.ne MBVc-6ccB 9> T.;< 0+<=- V./5 Nam, T.ne 9d6M-6ccB 5> "G nin .h1, "G ,o^n ,iGn, BYi q1c "&i, e*u#Dn Th Thnh, q]n* Th Thu, "G e*c TS. H:a 2.-+ 01-g -g+./7, eB \hoa hc v I thuRt H ei.

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6c

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