H19 lncRNA gene: o Mechanism: Expressed in high levels maternally in embryonic tissues = paternally imprinted Repressed after birth

th in both Transcripton only remains in skeletal muscle o Role: H19 RNA Involved in [myoblast] differentiation and regeneration of skeletal mscle Knockdown h19 RNA in myoblast cells via siRNA differentitation Knock out h19 mouse satellite cells -> differentitation o Encodes: miR-675-3p induced during skeletal muscle diferentitation miR-675-5p induced during skeletal muscle diferentitation o myoblast differentitation: H19 ---| myogenesis Exogenous miR-675-3p and miR-675-5p expression-- myogenesis o Skeletal muscle regeneration: H19 deficient mice - abnormal skeletal muscle regeneration following injury Exogenous miR-675-3p and miR-675-5p expression induce normal skeletal muscle regeneration miR-675-3p + miR-675-5p o mechanism: miR-675-3p ----| anti-differentitation Smad 1 and smad 5 - BMP pathway miR-675-5p ----| DNA replication inititation factor Cdc6 - DNA replication

CONC: h19lncRNA = important trans-regulatory function in skeletal muscle differentitaiton and regeneration -- microRNA enocoded in H19 exon1