TRNG I HC CNG NGHIP TPHCM

KHOA CNG NGH HA

BO CO TIU LUN MN PHN TCH THC PHM

TI:
XC NH GLUCOSE V FRUCTOSE T QU
TRNH NG PHN HA GLUCOSE BNG HPLC
XC NH HM LNG NG GLUCOSE V
FRUCTOSE TRONG NGUYN LIU MA BNG
PHNG PHP SC K TRAO I ANION
GVHD: Nguyn c Vng
Lp : DHPT6LT
Nhm : 06

Danhschnhm
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Hong Duy Ho
Nguyn Th Thu Hoi
Nguyn Ngc Hong
Trng Thng Hi
L Minh Hiu
Phan Hunh Tun Khoa
Phan Thanh Ph
Trn Vn Trnh
Nguyn Minh T

XC NH GLUCOSE V FRUCTOSE T QU TRNH

NG PHN HA GLUCOSE BNG HPLC
1. Gii thiu chung v glucid
2.Vi nt v glucose v fructose
3. Phng php xc nh
4. Xc nh glucose v fructose t qu trnh ng phn ha
glucose bng phng php HPLC vi u d UV

Gii thiu chung v glucid

1. Tng quan v glucid:
Lng glucid trong cc nguyn liu thc vt v ng vt rt khc nhau.
Trong thc vt, glucid l thnh phn ch yu, chim ti 85-90% trng
lng cht kh. ng v tinh bt c cha bn trong cc t bo cn
non, cn thnh t bo th c cc polysacchrid nh cellulose,
hemicellulose, protopectin...
Trong cc thc phm ng vt, thng lng glucid li rt t (thng
khng vt qu 2% so vi lng cht kh). Tht v trng c ri t
glucid, ch c, sa l tng i nhiu hn.
Ngun glucid m thc phm cung cp cho con ngi ch yu ly t thc
vt
Glucid c bn cht ha hc l polyhydroxy aldehyde hoc polyhydroxy
ketone. a s cc glucid c cng thc tng qut l (Cm(H2O)n). Ngoi ra
cn c mt s loi glucid c bit, trong cu trc ca chng ngoi C, H,
O cn c thm S, N, P.

Phn loi glucid

1. Theo cu trc ha hc:
-ng n (Mono saccharides): ng c 6 carbon ph bin nh ng
Glucoza, ng Fructoza, ng Galactoza...
-ng kp (Disaccharides): ph bin nh sucroza, lactoza, mantozo
-ng a (Polysaccharides): tinh bt, glycogen v cellulozo...
Hoc ta c th chia lm hai nhm:
- Nhm oza gm cc loi ng trc tip kh oxy do c nhm andehyt hay
xeton t do trong phn t. VD: glucoza, fructoza, latoza
- Nhm ozit, khng trc tip kh oxy do c nhm aldehyt v xeton di
dng kt hp vi nhm chc khc khi thy phn cho hai hoc nhiu oza.
VD: tinh bt, saccarozahoc khi thy phn ngoi cc oza cn cho cc cht
khng phi oza. VD: glucozit. Nhng glucozit khng c gi tr v dinh
dng, m l nhng cht c tnh cht dc l dng lm thuc cha bnh:
hoc l cc cht c.

Phn loi glucid

2. Theo tnh cht:
Glucid tinh ch: y l cc Glucid thng qua nhiu mc lm
sch v loi b ti a cc cht th km theo. T l cc Glucid tinh
ch trong thc phm cng cao, thc phm cng d tiu ha v
nhanh chng c s dng cung cp nng lng cho c th.
Glucid tinh ch cao c trong sn phm ng, bnh ko
Glucid bo v: thuc nhm ny l cc Glucid thc vt di dng
tinh bt c km theo lng Cellulo khng t hn 0.4%, v d nh
khoai ty, ng cc nguyn ht. Nhm Glucid ny chm tiu v rt t
c s dng to m.

Vai tr ca glucid
Glucid c vai tr rt quan trng trong c th sng:
Tham gia mi hot ng sng ca t bo.
L ngun cht dinh dng d tr d huy ng, cung cp ch
yu cc cht trao i trung gian v nng lng cho t bo.
Tham gia vo cu trc ca thnh t bo thc vt, vi khun; hnh
thnh b khung (v) ca nhm ng vt c chn khp.
Tham gia vo thnh phn cu to ca nhiu cht quan trng
nh: AND, ARN
i vi cng ngh thc phm, vai tr ca glucid cng a dng v
v cng quan trng:
L cht liu c bn, cn thit v khng th thiu ca ngnh sn
xut ln men: ru, bia, bt ngt, acid amin, vitamin, khng sinh. Tham gia to cu trc, hnh th, trng thi v cht lng cho cc
loi sn phm thc phm

Vai tr ca glucid
To kt cu:
To si, to mng, to gel, to c, cng, n hi cho thc
phm: tinh bt, thch, pectin trong min, mt qu, kem, gi la To kt cu c th ca mt s loi thc phm: phng n ca
bnh phng tm, to bt cho bia, xp cho bnh m, v chua cho
sa
To cht lng
Cht to ngt cho thc phm (cc ng) - Tham gia to mu sc v
hng thm cho sn phm (ng trong phn ng caramen ho,
melanoidin) - To ra cc tnh cht lu bin cho sn phm thc
phm: dai, trong, gin, do - C kh nng gi c cc
cht thm trong sn phm thc phm - To m cng nh lm gim
hot nc lm thun li cho qu trnh gia cng cng nh bo qun

Vi nt v glucose v fructose

1. DGlucose
2. DFructose

DGlucose
y l loi monosaccharid ph bin ng vt v thc vt. N
c nhiu trong nho chn nn cn c gi l ng nho.
Trong dung dch, D-glucose dng pyranose. D b ln men bi
nm men.
D- glucose l thnh phn c bn cu to nn nhiu loi
polysaccharid: tinh bt, glycogen, cellulose,.
CHO
H C OH
HO C H
H C OH
H C OH
CH2OH
D - glucose

CH2OH
O
H
H
H
OH H
OH
HO
H OH
D- Glucose

CH2OH
O
H
H
OH
OH H
H
HO
H OH
D- Glucose