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WALNE'SMEDIUMFORALGALCULTURES

Recommendedforlargevolumesofaquaculturestrains

Stocks

per100ml
(1) Tracemetalsolution(TMS)
ZnCl2
2.1g
CoCl2.6H2O
2.0g
(NH4)6Mo7O24.4H2O
0.9g
CuSO4.5H2O
2.0g
Make up to 100 ml with distilled water. This solution is normally
cloudy.Acidifywithafewdropsofconc.HCltogiveaclearsolution.
(2) Vitaminsolution
VitaminB12.(Cyanocobalamin)
VitaminB1 (Thiamine.HCl)
VitaminH(Biotin)
Makeupto100mlwithdistilledwater.

10.0mg
10.0mg
200.0g
perlitre

(3) Nutrientsolution
FeCl3.6H20
MnCl2.4H20
H3BO3
EDTA(Disodiumsalt)
NaH2PO4.2H2O
NaNO3
TMS(1above)
Makeupto1litrewithdistilledwater.

Medium
Nutrientsolution(3)
Vitaminsolution(2)
Sterilisedseawater

1.3g
0.36g
33.6g
45.0g
20.0g
100.0g
1.0ml

perlitre
1.0ml
0.1ml
1.0litre

mediarecipes

Dispensenutrientandvitaminsolutionsseparatelyinto10mland1mlrespectively
and autoclave at 15 psi for 15 minutes. Addan aliquot of each aseptically to 10
litresofsterilisedseawater.

Reference
WalnePR(1970)Studiesonthefoodvalueofnineteengeneraofalgaetojuvenile
bivalves of the genera Ostrea, Crassostrea, Mercenaria, and Mytilis. Fish. Invest.
26,162.

7August2002

CCAP(CultureCollectionofAlgaeandProtozoa),DunstaffnageMarineLaboratory,
Oban,Argyll,PA371QA,UK
Tel:+44(0)1631559000Fax:+44(0)1631559001Email:ccap@sams.ac.ukWeb:www.ccap.ac.uk