WHAT IS DNA RECOMBINANT?

Recombinant DNA (rDNA) is a form of

artificial DNA that is created by combining two or more sequences that would not normally occur together. In terms of genetic modification, it is created through the introduction of relevant DNA into an existing organismal DNA.

The recombinant DNA technique was first proposed by Peter Lobban, a graduate at the Stanford University Department of Biochemistry. The technique was then realized by Lobban and Kaiser; Jackson, Symons and Berg; and Stanley Norman Cohen, Chang, Herbert Boyer and Helling, in 197274.

WHAT IS DNA RECOMBINANT?

WHAT IS GENE CLONING?

Gene cloning is the process in which a gene

of interest is located and copied (cloned) out of DNA extracted from an organism. It is used by researchers to create copies of a particular gene for downstream applications, such as sequencing, mutagenesis, genotyping or heterologous.

The traditional technique for gene cloning involves the transfer of a DNA fragment of interest from one organism to a self-replicating genetic element, such as a bacterial plasmid.

WHAT IS GENE CLONING?

Steps in cloning a gene

DNA (TARGET GENE) RESTRICTION ENZYMES DNA CLONING VECTOR DNA LIGASE HOST CELLS

DNA(TARGET GENE)
Targeted Gene Therapies- Provides

correction to the template for the homologdirected repair. Targeted Gene Therapies enables the cells own repair, erase the mutation and replace it with the correct sequence.

DNA( TARGET GENE)

 Allows

foreign genes to be added to an existing organisms. Example: Adding new genes to create plants more resistant to pest and more tolerant to drought. Examples of restriction enzymes are EcoRI which always cuts DNA at the DNA sequence GAATTC.

RESTRICTION ENZYMES

DNA CLONING VECTOR

Cloning vector - a DNA molecule that

carries foreign DNA into a host cell, replicates inside a bacterial (or yeast) cell and produces many copies of itself and the foreign DNA. Types of cloning vectors used are genetic engineered Plasmids and Bacteriophages.

DNA CLONING VECTOR

DNA Ligase are used with restriction enzymes to insert DNA fragments, genes into plasmids.

DNA LIGASE-CONTINUATION

 Transduction-

A process by which DNA is transferred from one bacteria to another by a virus. Transduction introduces a foreign gene into a host cell.

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