H THNG REAL-TIME PCR M TI LNG VIRUS CHO PHNG

XT NGHIM SINH HC PHN T

1. Cc h thng Real-time PCR trn th trng
Hin nay trn th trng c 2 dng my chnh l: H thng ng (t ng) v h
thng m (bn t ng). Mi h thng u c u v nhc im ring.
H thng ng bt buc phi s dng ha cht v vt t c quyn ca hng,
chi ph rt t i a s bnh nhn ca Vit Nam khng c iu kin chi tr cho chi ph
xt nghim. H thng ny thc hin c rt t tc nhn (HBV, HIV, HCV, Lao). u
im ca h thng ny l t ng hon ton trnh c sai st do con ngi v t
chnh xc cao.
H thng m c th s dng c nhiu loi kit thng mi trn th trng
ngi dng cng c th t chun ha cc ha cht xt nghim m KHNG PH
THUC VO NH CUNG CP nn chi ph xt nghim rt r v ph hp i a s
ngi dn Vit Nam. Nhc im ca h thng ny l t chnh xc tng
ng vi h thng ng, i hi i ng k thut vin lnh ngh cng nh cn b
phn tch c chuyn mn v kinh nghim.
Ngoi ra, h thng m khch hng c th trang thm my tch chit ADN t
ng thay cho vic tch chit ADN th cng gim s sai st ca yu t con ngi.
Tuy nhin h thng ny i hi ha cht v vt t tch chit ADN i km theo my l
c quyn ca nh sn xut nn rt t, tng t nh h thng ng trn.
Cc kit tch chit ADN ca cc hng hin nay quy trnh rt n gin, thao tc t
nn vi i ng k thut vin c tp hun tt hon ton c th m bo cht lng
tng ng vi h thng tch chit t ng. Do , thi gian u vi lng mu
trung bnh (khong 500 XN/thng) khng cn trang b h thng tch chit t ng.
2. T vn h thng Real-time PCR ph hp cho phng th nghim chn on
H thng dng cho mc ch m ti lng virus, v pht hin cc cn nguyn
nhim trng bng phng php sinh hc phn t. Ngoi ra h thng c th thc hin
c cho pht hin cc t bin gene gy ung th, pht hin cc bnh di truyn.
H thng real-time PCR m dng trong bnh vin phi p ng cc tiu chun
c bit quan trng sau:
Block nhit thit k ph hp vi cc loi tube thng dng trn th trng hin
nay(PCR 0.2ml, trip 0,2ml)
Th tch phn ng ca h thng c thit k ph hp vi cc kit thng mi
hin nay l t 25 l 50 l, th tch nh hn 25 l l khng ph hp. Th tch
mu < 25 l/phn ng thng khng t nhy cho mc ch chn on v d
xy ra sai st thao tc pippeting.
H thng knh lc phi thng dng, s lng knh knh lc (Tc nhn pht hin
c ng thi) phi 2. Nn chn cc h thng c 4 hoc 5 knh knh lc
c th s dng cc kit Multip-plex Real-time PCR hin nay trn th trng.
B lu in s dng cho h thng Real-time PCR phi s dng c trn 2h
m c th chy xong 1 quy trnh phn ng trong trng hp mt in. Quy
trnh chy my phi lin tc, ch gin on 1 giy khi chy my cng phi tin
hnh li l th nghim. B lu in c cng sut ph hp nhm m bo vic tr
kt qu cho bnh nhn kp thi cng nh gim thiu thit hi khi s c mt in
xy ra.
S dng c hu ht cc kit thng mi trn th trng pht hin cc cn
nguyn truyn nhim ph bin nh: Virus vim gan B (HBV), virus vim gan C
(HCV), vi khun lao, vi rt HPV l nguyn nhn gy bnh ung th c t cung
ph n, vi rt chn tay ming (EV/EV71), Rubella, cc virus gy bnh da liu,
bnh nhim khun sinh dc d gy v sinh do chlamydia, cc virus gy nh
hng ti thai nhi trong thi k ngi m mang thai nh Cytomegalovirus
(CMV), virus gy bnh c quan sinh dc v gy v sinh Herpes (HSV), virus gy
tng bch cu n nhn EBV,.
3. H thng real-tim PCR c cung cp bi cng ty B.D.E bao gm:
H thng Real-time PCR nh lng ABI 7500 Apply Biosystems (M), l h
thng m ph hp vi hu ht cc kit thng mi trn th trng, ngi s dng
KHNG PHI PH THUC VO NH CUNG CP. H thng c thit k ph
hp cho mc ch chn on vi 5 knh kch lc, th tch phn ng ti u t
25l 50l hon ton ph hp cho mc ch chn on.
Bnh vin hon ton c th la chn ha cht ca nhiu hng khc nhau hoc
t ti u vi chi ph thp ph hp cho i a s bnh nhn ngho ti Vit Nam.
B lu in 1,4 KAV s dng cho ton h thng ln n trn 2 ting, m bo
cho qu trnh chy ton b quy trnh khi c s c mt in xy ngay khi mi bt
u chy.
Thit k v cung cp MIN PH 01 h thng bn th nghim tiu chun cho
phng xt nghim sinh hc phn t vi vt liu composite to nn khng gian
chuyn nghip cho 1 phng xt nghim k thut cao.
Cam kt chuyn giao k thut xt nghim cc cn nguyn nhim trng nh nh
tnh, nh lng, nh type HBV, HCV, HPV, Lao EV, EV 71