Centrifugation

1cp 0.00125
10 0.00125
Viscosity 1.25 m*Pa*s
Viscosity (SI) 0.00125 Pa*s
Density difference 1 kg/m^3
diameter 0.00005 m
g 9.81 m/s^2
Terminal velocity 0.00018611 m/s
v_terminal 67 cm/hr
Volume from bioreactor feed 1400 L
Volume concentrate 21*times 66.6666667 L
Volume Waste 1333.33333 L
Pa*N
Two step Filtration Process
Filtration
Retentiate
Diafilter 7*reduction
Buffer Addition
Buffer Out
Final Product
after Filtration
21*reduction Flux
Area*Time
Time
Area
Total Volume
Flux
7*reduction Area*time
Time
Area
Total Area
Each Membrane Area (300mm*300mm)
Number of Membrane Required
Total Time For the process
Two step Filtration Process
66.66667 L
240 L
240 L
1333.333 L
28211 LMH
21.15825 m
2
*hr
7 hr
3.022607 m
2
306.6667 L
28211 LMH
0.01087 m
2
*hr
1 hr
0.01087 m
2
3.033478 m
2
0.09 m
2
33.70531
8 hr
Environmental aspect Risk
Waste aqueous cell culture Contains different chemicals and
biomass wastes which are toxic
Safety and health Aspect
Chemical Risk
PS9 insect cell is stored with 7-13% Skin soluble. Hazardous to skin and
of dimethyl sulfoxide (DMSO) also cause eye irritation
which is an hazardous chemical
Sodium bicarbonate (buffer)
Virus infection by deadly H1N1, H3N2,
and H5N3 virus
mask for nose and safety glasses for eye protection.
Action taken
Disposed safely with price provided
in the problem statement
Action taken
Use of gloves and clothing while handling
Storage: Cool ventelated area.Also, quick access to
safety shower in case of bodily contact.
Use of gloves and clothing while handling
Working on bio safety level-3 working zone with
head to toe covered with the lab clothing including
mask for nose and safety glasses for eye protection.