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1. Introduction
Cells are dynamical systems characterized by a high degree of complexity due to an intricate
network of subcellular processes that sustain their correct performance. This network is
formed by a series of subnetworks with modular functioning, and among them is the
subnetwork of biochemical processes that produces cell death or apoptosis. In this chapter
we deal with the dynamical behavior of the apoptotic pathway activated by ionizing
radiation (IR). In the special case of the apoptotic process, mathematical modeling is
required for understanding its nonlinear complex behavior. Although previous attempts to
model apoptosis were often limited to small systems, or based on qualitative data only,
recent models are based on experimental data that give support to the theoretical results.
In this form, mathematical models have shown that the interactions between the nuclear
molecular components of the intrinsic apoptotic pathway drive the system into a persistent
limit cycle in the respective phase space after the application of high doses of ionizing
radiation. Experimental data confirm the existence of this limit cycle, showing that this
dynamical feature of the system relies on the existence of a p53-Mdm2 negative feedback
loop, which is induced by a switch-like activation of the ATM kinase.
At the cytoplasm, the molecular dynamics that leads to the activation of the caspases sets up
a saddle point bifurcation in the caspase 3-Bax degradation rate diagram. In this region of
bistability, the saddle point bifurcation can drive the system either to a stable point with a
high concentration of caspase 3 (cell death) or to a stable point with a low concentration of
caspase 3 (cell survival). Out of this region, the system dynamics is settled on by the
existence of a single stable fix point, which determines cell surviving independently of the
concentration of caspase 3, i.e., there exits a threshold mechanism that regulates apoptosis
and it is based on bistability, ultrasensitivity and irreversibility.
Thus, the main goal of this chapter is to review and analyze the recent knowledge about these
nonlinear dynamical aspects of IR-induced apoptosis, in order to understand how this
complex subnetwork of molecular interactions integrates the information coded in the signals
that control the cell survival, and executes the corresponding action to determine the correct
death-life decision. The modeling of such decisions has a significant impact on the field of
radiation biology and in the therapeutic aspects of inducing apoptosis in cancer cells by IR.
complexity of cellular process that it entails. A deeper insight into the causes of cancer
progression, involves further research on the cell cycle because the malignant
transformation starts as a deregulation of this cycle. In cancer, serious errors can be
observed in DNA replication and repair simultaneously with an uncontrolled cell growth.
Cancer cells lack the ability to respond to internal or external stimuli that induces death
(Malumbres and Barbacid, 2009). It has seen that between 50 and 70% of all cancer types are
related to the absence of the transcriptional factor p53 (Vousden, 2006), which serves to
activate the cellular repairing response to DNA damage.
it will interact with the ubiquitin ligase Mdm2 (Hdm2 in humans). Mdm2 binds to p53,
forming a complex that eventually will be degraded in the proteosome (Jänicke et al., 2008).
It is noteworthy that at the basal level the Mdm2 gene promoter is activated by transcription
factors other than p53, which have not yet been identified (Phelps et al., 2003). It is known
that the Mdm2 gene has two promoters (P1 and P2) that can lead to at least two isoforms of
Mdm2 (Candeias et al., 2008). The p90Mdm2 isoform is responsible of the inhibition of p53,
and the p76Mdm2 isoform is a p53 activator (Perry, 2004) and promotes the translation of
p53 mRNA (Candeias et al., 2008). Under basal conditions p76Mdm2 expression is greater
than p90Mdm2 (Phelps et al., 2004), and this fact could explain the origin of the basal levels
of p53 throughout the cell cycle (Figure 1a). However, under cellular stress conditions, p53
induces the expression of p90Mdm2 in detriment of the p76Mdm2 isoform (Perry, 2004).
Fig. 1. (a) p53 network at basal conditions. p53 and other transcription factors, some of
them no yet identified and marked as X, activates p53 gene. Part of the novel p53
synthesized interacts with BCL-XL, forming heterodimers that will remain in the cytoplasm.
The other part of p53 molecules is marked with an importation signal, enters the nucleus,
and interacts with p90Mdm2 (represented by “Mdm2”) forming a complex that degraded in
the proteosome. “Y” represents a transcription factor set that activates p90Mdm2 in absence
of genotoxic stress, and the empty set symbol represents protein degradation. (b) ATM
activation network in response to DNA damage. DNA damage causes a nuclear topology
change that induces the self-phosphorylation of ATM homodimers (represented by
ATM/ATM), allowing its separation into active monomers (represented by ATM-on). The
ATM activation is full after that TIP60 acetylates it. In the other side, if MRN complex
(forming by Mre11, Rad50 and Nds1) detects broken DNA, it will be activated (represented
by MRN-on) and then it will bind to broken DNA, in order to stop the damage propagation.
After ATM activation, it will phosphorylate various substrates including Nbs1, and this
permits ATM union to MRN-broken DNA complex, in order to start the DNA damage
repair signaling pathway, through activation of effector proteins like H2AX.
intensity and nature of the aggression. In particular, if the cell undergoes genotoxic stress
there are three possible scenarios, and multiple combinations of them. The first scenario is
when the cell suffers minor levels of DNA damage and can repair it successfully. The second
scenario is when the cell suffers a medium damage in its DNA, and the cell has to “decide”
between life and death. In this scenario it is possible that the cell die for not being able to
fully repair the damage after a finite period of time, or live for being able to repair it. The
third scenario is when the cell suffers severe damage, and initiates the apoptotic death. For a
cell can be able of executing one of these three different responses, DNA damage intensity
must be first detected, and then, the cell must “make the choice” of the appropriate scenario.
Fig. 2. (a) Module of ATM effector functions. When ATM is anchored to MRN-broken DNA
complex, ATM phosphorylates Chk2 inactive monomers, to promote its binding into active
homodimers. After that, free active ATM with Chk2 active homodimers will phosphorylate Rb
protein to release E2F1. This transcription factor controls the pro-apoptotic protein synthesis,
such as ASPP proteins. Free active ATM can bind to NEMO to induce NF-kB release in the
cytoplasm. It is important because NF-kB is responsible to regulate the expression of proteins
required to cell survival, like BCL-XL. In figure the stepped line represents the induction of
protein transcription. (b) The p53 activation by ATM and Chk2. After the activation of ATM
and Chk2, ATM inhibits Mdm2. Together with Chk2, ATM phosphorylates p53 in order to
stabilize it. Phosphorylated p53 can bind either to MUC1 to promote the transcription of genes
related with the cell cycle arrest and DNA damage repair, or can bind to ASPP proteins to
promote the transcription of genes related with apoptosis.
Dynamical Aspects of Apoptosis 247
In the nucleus, ATM interacts with NEMO forming a complex, which is ubiquitinated
(Figure 2a), and transported to the cytoplasm where it induces the release of the
transcription factor NF-kB (Schmid and Birbach, 2008). After that, NF-kB enters the nucleus
and promotes the expression of various genes, including BCL-XL (Méndez et al., 2006; Chen
et al., 2000). Simultaneously, ATM phosphorylates p53 nuclear stockpile, and, as a result,
Mdm2 becomes less effective to recognize p53, delaying its degradation. Also, ATM
phosphorylates Mdm2, inducing its autoubiquitination and degradation in the proteosome,
and allowing an increase of p53 nuclear concentration over time (Dogu and Díaz, 2009).
After ATM phosphorylation, Chk2 phosphorylates p53 in order to stabilize its structure
(Pommier and Weinstein, 2006). At the end of this initial chain of phosphorylation, p53 can
interact either with ASPP1 and ASPP2 proteins or with MUC1 (Pietsch et al., 2008). The
MUC1 transmembrane glycoprotein is cleveaged in response to a genotoxic stimuli and the
cytoplasmic segment is targeted to the nucleus, allowing its union with phosphorylated p53
and the induction of cell arrest (Pietsch et al., 2008). On the other hand, in response to severe
DNA damage (Figure 2b), p53 will bind mainly with ASPP proteins. The concentration of
the ASPP proteins increases gradually due to the activity of E2F-1 (Pietsch et al., 2008;
Braithwaite, 2006), enhancing the pro-apoptotic function of p53. In this form, depending on
the intensity of the stimulus, p53 can be oriented to transcribe pro-apoptotic genes or anti-
apoptotic genes, depending on the accessory proteins to which it is attached.
2.4.2 Soft DNA damage, cell cycle arrest and successful repair of damage
When the DNA damage signal is weak (Figure 3b), MUC1 nuclear concentration increases,
and the union between p53 and nuclear MUC1 is enhaced. After that, the complex p53-
MUC1 interacts with PCAF (p300/CBP associated factor) to form a new complex (Pietsch et
al., 2008). PCAF acetylates p53 on lysine 320, allowing it to specifically recognize the
promoters of genes related with the cell cycle arrest like p21WAF1/cip1, and DNA damage
repair (Pietsch et al., 2008). Similarly, SIRT1 opposes PCAF-dependent phosphorylation of
p53, blocking its transcriptional activity when the cell does not have energy to express
proteins (Yamamori et al., 2009; Pediconi et al., 2009; Ikenoue et al., 2008). However, SIRT1 is
not the only control point of p53. As discussed above, ATM, along with NEMO, activates
NF-kB in its alternative pathway. This interaction is important, because NF-kB promotes the
expression of anti-apoptotic genes like BCL-XL. NF-kB also competes with p53 for the
cofactors: acetyltransferases p300, CBP and PCAF; which are required for the binding of p53
248 Current Topics in Ionizing Radiation Research
to DNA (Jänicke et al., 2008). The effect of this interaction leads to the indirect repression of
the transcriptional activity of p53, given the fact that the concentration of the cofactors
remains constant (Schmid and Birbach, 2008; Youle and Strasser, 2008).
Fig. 3. (a) The onset of apoptosis in its intrinsic pathway. In response to severe DNA
damage, p53 will bind to ASPP1 and ASPP2 (all of these are represented by ASPP). After
that, p53-ASPP complex will interact with p300/CBP, and p300 acetylates p53-ASPP
complex (represented by p53-ap). This allows p53-ap recognize the promoter side of
apoptotic genes, like PUMA and BAX. The p53-ap transcriptional activity increases PUMA
levels in the cytoplasm sufficiently to induce the release of p53 from p53/BCL-XL
complexes. Free cytoplasmic p53 releases BAX in the cytoplasm starting C cytochrome-
dependent apoptosis. (b) Activation of cell cycle arrest and DNA damage repair pathways.
In response to medium intensity DNA damage, p53 will bind to MUC1. The p53-MUC1
complex interacts with PCAF (represented by p53-cl) to allow the expression of genes
related with cell cycle arrest like p21, as well as the expression of genes related with DNA
repair (in the figure are represented by “Z”). Another remarkable function of p53-cl is
inducing the Wip1 and Mdm2 expression. Wip1 is a phosphatase that down-regulates the
p53 network.
Among the p53-target genes that are activated in this pathway are WIP1 phosphatase and
Mdm2 in its p90 isoform. The Wip1 function is to dephosphorylate Chk2, ATM, activated
p53 and Mdm2 (Figure 4). This leads to inactivation of all of these proteins except Mdm2,
which is activated (Braithwaite, 2006; Candeias et al., 2008; Kobet et al., 2000; Lahav et al.,
2004; Jänicke et al., 2008; Bernards, 2004). The dephosphorylated form of Mdm2, activated
WIP1, and new synthesized Mdm2, inhibit p53 reducing its nuclear concentration (Hu et al.,
2007). Because the WIP1 function, the signal generated by ATM and Chk2 will blink while
DNA damage is not repaired, since ATM activator proteins remain linked to broken DNA
(Jänicke et al., 2008; Lu et al., 2008). When DNA damage is repaired nuclear topology is
restored, and ATM activating proteins trigger stop signal. In this case, Wip1 definitely
inactivates ATM, and effector molecules like Chk2. This encourages Mdm2 (p90 isoform) to
efficiently suppress p53 activity (Figure 4). These interactions allow the cell to return to its
basal state, and the levels of p90, Mdm2 and Wip1 also return to their basal value (Bernards,
2004).
Dynamical Aspects of Apoptosis 249
Fig. 4. Mdm2 and Wip1 down-regulation of p53 network. The main targets of Wip1 are
ATM, Chk2 and Mdm2 phosphorylated. This results in a silencing of the ATM signal, and
the Mdm2 activation. Mdm2 inhibits p53, in order to return its levels to normality, and
restored the network equilibrium. In figure red lines represent the interactions that blocks
Wip1.
c k x , t
rk r c1 x , t c 2 x , t ...c k x , t ...cs x , t Dk 2c k ( x , t ) (1)
t r
which means that the local rate of variation of the concentration of the substance k (denoted
by c k ) at point x at time t is equal to the net rate of diffusion of k inside the cell volume V
(denoted by Dk 2c k x , t ) plus the rate of formation/degradation of k due to the local
chemical reactions inside V. In equation (3.1), r represents the local rate of the chemical
reaction r, which is a functional of the concentration of the reactive substances at point x at
time t, and rk is the stoichiometric coefficient of k in reaction r.
Dynamical Aspects of Apoptosis 251
c k x , t
f k c1 x , t , c 2 x , t ,..., c k x , t ,...cs x , t Dk 2c k ( x , t ) k 1, 2,..., s (3)
t
which is the well known form of the reaction-diffusion equation for the substance k. It
indicates that the temporal variation of the concentration of k at point x at time t depends on
the balance between the chemical processes in which this substance takes part, represented
by the function fk, and its diffusion rate in the cellular medium. Function fk is generally a
nonlinear function of the concentration of the reactive substances, and equation (3) usually
has not an analytical solution. In a homogeneous medium the diffusive term in equation (3)
is null, and fk completely defines the entire system dynamics in the s-dimensional phase
space, which is defined by the set of concentration values of the s reactive substances. The
systems dynamics is represented by a trajectory in this space, defined by the column vector
c(t ) c1 t c 2 t ...cs t . In nonlinear systems this trajectory can have peculiar
characteristics like high sensitivity to initial conditions, bifurcations and complex loops that
represent a great variety of dynamical behaviors observed in biological systems like limit
cycles, hysteresis, bistability, ultrasensitivity, among others. In not homogeneous medium
the diffusive term of equation (3) produces a more complex dynamical behavior of the
system, giving rise to phenomena like traveling waves, spirals and spatially located bursting
of second messengers and proteins, among others.
In the subsequent sections it is discussed in depth the nonlinear dynamics of homogenous
chemical systems.
f 1 c1 t , c 2 t ,...c k t ,...cs t
c t f c t , where f c t (4)
f s c 1 t , c 2 t ,...c k t ,...cs t
becomes cero. Once the set of steady points of the system is settled on, is necessary to
determine their stability. Equation (4) subject to the initial condition c 0 c0 defines a
nonlinear dynamical system.
The steady point co of a dynamical system is Liapunov stable if for each > 0 exits a > 0
such that c t co whenever c 0 co , i.e., any trajectory that initiates at a distance
o
of the steady point c remains at a distance of it for all positive time.
252 Current Topics in Ionizing Radiation Research
initiates at a distance of the steady point co will converge to it eventually. In this case, the
point co is an attractor of the dynamical system in the phase space. A steady point co , which
is Liapunov stable and attracting, is asymptotically stable.
A steady point co , which is neither stable nor attracting, is unstable and is a repulsor in the
phase space.
f 1 f 1
c1 c1 f 1 (c1o , c 2o )
c 1
c1
c 2
c 2 O 2c1 , 2c 2 , c1 c 2
c o
,c o
c o
,c o
1 2 1 2
(5)
f f
1
c1
c1 1
c 2
2
c 2 O c1 , c 2 , c1 c 2 2
because f 1 (c1o , c 2o ) 0
c o
1 ,c 2o c o
1 , c 2o
Dynamical Aspects of Apoptosis 253
in a similar form:
f 2 f 2
c2 c2
c1
c1
c 2
c 2 O 2c1 , 2c 2 , c1 c 2 (6)
c o
1 ,c o
2 c o
1 ,c o
2
which leads to the linearized dynamical system:
f 1 f 1
c1 t c1 c1o ,c 2o c 2
c1o ,c2o c1 t c1 t
J c 1 , c 2 (7)
c 2 t f 2 f 2 2
c t c 2 t
c1 c1o ,c 2o c 2 c o ,c o
1 2
J c1 , c 2 represents the Jacobian matrix of the linearized dynamical system in equation 6. The
eigenvalues 1 , 2 of J c1 , c 2 can be calculated from the characteristic equation:
J c1 , c 2 I 0 . Depending on the nature of the eigenvalues, it is possible to know the
arrangement of the trajectories near each steady point of the nonlinear system (Figure 6).
This linearization process can be extended to perform the phase space analysis of higher
dimensional nonlinear dynamical systems (Edelstein-Kesher, 2005).
An important question that arises at this point is whether the behavior of the trajectories
obtained from equation (7) accurately reflects the real behavior of the trajectories of the
original nonlinear system. Andronov et al. (1973) show that this is the case. If the linearized
system has a saddle, a node or a spiral at a given steady point, then the original nonlinear
system really has also a saddle, a node or a spiral at that steady point. Furthermore, if a
steady point is a stable saddle or node of the linearized dynamical system, then is also a
stable saddle or node of the nonlinear system. In this case, the neglected nonlinear terms of
equations (5) and (6) practically have not effect on the stability of these points when Re()
0 for both eigenvalues. This kind of steady points is known as hyperbolic points, and they
are not affected by the small nonlinear perturbations. The topological implication of this fact
is that the vector field corresponding to a saddle or a node is not altered by small nonlinear
perturbations and, as consequence, has structural stability (Strogatz, 1994).
When the eigenvalues of the Jacobian matrix are pure imaginary = i , the steady point is
a center. The trajectories around this point are closed orbits that are stable. However, the
neglected nonlinear terms in equations (5) and (6) can produce an imperfect closure of the
orbit, giving rise to a spiral. In this form, the vector field corresponding to a center is altered
by small nonlinear perturbations that transform the center into a spiral and, as consequence,
has not structural stability (Strogatz, 1994).
According to their stability, steady points of 2-dimensional dynamical systems can be
classified into a: 1) Robust case, which includes repellers or sources, for which both
eigenvalues have Re() > 0; attractors or sinks, for which both eigenvalues have Re() < 0
and saddles, for which one eigenvalues has Re() > 0 and the other one has Re() < 0; 2)
Marginal case, which includes centers for which both eigenvalues are pure imaginary, and
non-isolated steady points for which one eigenvalue has Re() = 0 (Strogatz, 1994).
However, the phase space of a nonlinear system can exhibit another kind of closed orbits
called limit cycles, which cannot be observed in linear systems. A limit cycle is an isolated
254 Current Topics in Ionizing Radiation Research
field c f (c ) that is continuously differentiable on an open set of the plane containing R and
2) R does not contain any fixed point (Edelstein-Kesher, 2005). A consequence of this
theorem is that in a 2-dimensional phase space any trajectory trapped into a closed bounded
region R must converge to a limit cycle.
However, in higher dimensional systems the Poincaré-Bendixon theorem does not longer
apply and trajectories can be trapped into a closed region of the phase space without
converge into a limit cycle or settle down to a fixed point, and they could be attracted by a
complex geometric object called strange attractor, which is a fractal set on which the motion
is aperiodic and sensitive to very small changes in initial conditions. This sensitivity makes
the motion unpredictable as t increases, giving rise to a chaotic dynamics (Strogatz, 1994).
3.3 Bifurcations
The qualitative features of the vector field of a biochemical dynamical system are strongly
dependent on the set of parameters of its corresponding set of differential equations. As the
value of one of these parameters changes, the qualitative features of the vector field undergo
local variations around the steady points. This parameter-dependent change in the local
topological structure of a vector field is known as bifurcation. They generally occur in a one-
dimensional subspace, and the rest of the dimensions of the phase space are affected as
consequence of the flow that can be attracted or repelled from this subspace (Strogatz, 1994;
Edelstein-Kesher, 2005). Taking into consideration the imaginary plane, we can roughly
classify bifurcations into two cases: 1) the eigenvalues of the Jacobian matrix are both real
and bifurcations occur along the real axis as certain parameter changes. This kind of
bifurcation comprises the saddle-node bifurcation; the transcritical bifurcation, and the
subcritical and supercritical pitchfork bifurcation. 2) The eigenvalues of the Jacobian matrix
are complex conjugated. Bifurcations occur crossing the imaginary axis as certain parameter
changes. This kind of bifurcation comprises the supercritical and subcritical Hopf
bifurcation.
In the first case, a) the saddle-node bifurcation causes local variations in the vector field
around two points: a saddle and a node, as a bifurcation parameter changes. These points
become closer as parameter varies until they collide and annihilate each other. This type of
bifurcation has interesting applications in some models of biological processes that imply
the presence of chemical switches; b) a transcritical bifurcation occurs when two steady
points interchange their stability as the bifurcation parameter varies. c) The normal form
of an ordinary differential equation (ODE) that exhibits a subcritical pitchfork bifurcation is
c c c 3 . When <0 the steady point c = 0 is stable and there are two unstable points at
c . When > 0 the only real steady point c = 0 becomes unstable. The normal form
of an ODE that exhibits a supercritical pitchfork bifurcation is c c c 3 . When < 0 the
only real steady point c = 0 is stable. For > 0 there is an unstable steady point at c = 0 and
to stable steady points at c .
In the second case, the presence of a Hopf bifurcation leads the system to a limit cycle. As
the bifurcation parameter varies, when a certain critical value c is reached the
supercritical Hopf bifurcation drives the transformation of a stable spiral into an unstable
spiral that converges to a stable limit cycle (Figure 7). The case of a subcritical Hopf
bifurcation is more complicated. A typical example is when an unstable limit cycle shrinks
to cero amplitude as the bifurcation parameter reaches its critical value c, at which the
256 Current Topics in Ionizing Radiation Research
cycle engulfs the node rendering it unstable and making the system to jump to a distant
attractor when > c (Strogatz, 1994; Edelstein-Kesher, 2005). This new attractor could be a
steady point, another limit cycle, infinity or a chaotic attractor (for higher dimensional
systems).
Fig. 7. Supercritical Hopf bifurcation. This kind of bifurcation transforms a stable spiral
into an unstable spiral that converges to a stable limit cycle when the value of the
bifurcation parameter reaches some critical value c. The black points in the figure are
different initial conditions of the dynamical system. The arrows mark out the direction of
the flow of the vector field. The phase space is spanned by c1(t) and c2(t).
Dynamical Aspects of Apoptosis 257
When -radiation or the radiomimetic drug neocarzinotastina (NCS) are applied to a cell, the
output is digital. According to the results of Batchelor et al. (2008, 2011), the DSB’s produced
by these agents induce the onset of a limit cycle of fixed duration, amplitude and period (~ 5
h) in the p53-Mdm2 phase space. The number of pulses of both molecules increases as the
damage increases. This series of pulses have the characteristic of an “all or none” response
in single cells, which is trigged even by transient inputs (Batchelor et al., 2008).
A previous model by Ma et al. (2005) predicts this kind of digital dynamics under two basic
facts: 1) the number of DSB’s for a given doses is stochastic and follows a Poisson
distribution with an average proportional to the radiation dose applied x and given by 35x,
which is consistent with the observed 30-40 DSB’s per Gy. The repair dynamics is simulated
with a Monte Carlo method; 2) the repairing of the DNA damage is a biphasic process, i.e.,
consists of a rapid and a slow phases of DSB fix, which are assumed to be produced by two
different types of DSB’s. The simple DSB’s, which contain a break in each strand of DNA of
a short segment of 10-20 bps in length, switches on the fast repairing process. The slow
repairing process is trigged by complex DSB’s, which contains breaks in each strand of
DNA, together with other kind of alterations like base damage, base deletions, etc., within
the same short segment. The fast and slow processes are represented by different chemical
kinetics (Stwart, 2001).
Fig. 8. (a) Experiments with fluorescent p53 and Mdm2. Two pulses of p53-CFP (green) and
Mdm2-YFP (red) are shown in this panel. Time (in min) after irradiation is show below
images. In the figure, a phase difference of ~ 120 min between the p53 and Mdm2 maximum
fluorescence is observed. (b) Levels of p53-CFP and Mdm2-YCP in the nucleus of the cell of
panel (a). AU, arbitrary units. Figure reproduced from Lahav et al. (2004), with
authorization of Dr. U. Alon.
258 Current Topics in Ionizing Radiation Research
As mentioned above, the predicted dynamics from this model is a digital output, in which
doses of the order of 5 Gy (~ 150 DSB’s) applied in an on – off form produces pulses of
p53 and Mdm2 with a period of ~ 7 hr and a phase difference of ~ 115 min. These results
are consistent with the experimental results of Lahav et al. (2004) that are showed in
Figure 8.
In order to study the behavior of the p53 and Mdm2 loop in individual MCF7 breast cancer
cells, Lehav et al. (2004) fused p53 with the cyan fluorescent protein (CFP) under a zinc-
inducible promoter, and Mdm2 with a yellow florescent protein (YFP) under the human
Mdm2 native promoter. p53-CFP was completely functional in induced the transcription of
Mdm2 after zinc induction. The p53 and Mdm2 levels were recorded using time-lapse
fluorescent microscopy of the cell line clone expressing both fluorescent proteins after -
irradiation at 20 minutes resolution during 16 hrs of growth (Figure 8). The results show
that individual cells exhibit sustained oscillations in the p53-CFP and Mdm2-YFP nuclear
levels in response to DSB’s (Figure 8), but that the number of pulses varies from cell to cell.
The width of each pulse was of 350 160 min. The maximum of the first peak appeared 360
240 min after DSB, and the time between successive peaks stabilized at a 440 100 min.
The time delay between the maximum levels of p53 and Mdm2 was ~ 100 min. However,
some individual cells did not show pulses of p53 and Mdm2 after -irradiation.
The coincidence between the outputs from the models of Batchelor et al. (2008, 2011) and Ma
et al., (2005) with their own and Lehav et al. (2004) experimental results, indicates that -
irradiation produces DSB’s that induces the onset of a limit cycle through a supercritical
Hopf bifurcation in the p53-Mdm2 phase plane (Batchelor, 2008), and that the molecular
mechanisms that underlay this dynamical behavior possibly lies on the p53-Mdm2 negative
feedback loop (Figures 1a). However, the only existence of this loop seems not to be enough
to explain the sustained oscillations observed in individual cells (Lehav et al., 2004; Ma et al.,
2005; Batschelet et al., 2008 , 2011).
According to the results from the model of Ma et al., (2005), the presence of DSB’s induces a
switch-like behaviour of the ATM kinase, which dissociates from its inactive dimmer form
into two activated monomers that phosphorylate p53 (Figures 2a). This switch is turned on
by doses of -irradiation as low as 0.1 Gy ( 3 DSB’s) and saturates at 0.4 Gy ( 14 DSB’s).
Furthermore, activated ATM monomers (represented by ATM*) close a positive feedback
loop acting on the ATM inactive dimmer form to cleavage it and produce more activated
monomers. The combination of these two feedback loops is able of producing the sustained
oscillations observed in individual cells.
However, experimental and theoretical analysis of the p53-Mdm2 loop by Batchelor et al.
(2011) show that the sustained oscillations observed in individual cells can also be explained
by the action of a triple negative feedback loop. ATM* activates p53, which closes the first
negative feedback loop with Mdm2. However, p53 also activates the transcription of wip1
that closes a negative feedback loop with p53. Although, p53 also closes a negative feedback
loop with ATM* mediated by Wip1 (Figures 4). Experimental and in silico results (Batchelor
et al., 2008, 2011) show that the suppression of the negative action of Wip1 on ATM*
switches p53 dynamics from repetitive pulses into a single analog pulse.
Probably, the positive ATM feedback loop together with a initial rapid degradation of
Mdm2 (Batchelor et al., 2011) and the triple negative feedback loops mediated by p53,
Mdm2 and Wip1 accounts for the onset and stability of the limit cycle that gives rise to the
digital response to -irradiation observed in individual cells.
Dynamical Aspects of Apoptosis 259
Digital response of the p53 machinery to DSB by -irradiation could be a timing mechanism
that controls downstream events that determine if the DSB’s can be repaired or not (Figure
3a), and, in consequence, if apoptosis should continue until cell death or not (Ma et al., 2005;
Dogu and Díaz, 2009).
constant in these cells. In contrast, only 30% of the cells exposed to 5 Gy of -irradiation
showed sustained pulses, indicating that the number of cells displaying oscillations
increases with higher doses of radiation. Initially, the onset of the oscillations in different
cells was synchronized with the pulse of -radiation applied. However, synchrony was lost
due to the different frequency response exhibited by individual cells. Some cells stopped
their oscillatory dynamics or changed their frequency of oscillation. Additionally, after cell
division, sister cells exhibited oscillations of p53 and Mdm2 that were initially synchronized.
However, after a lapse of time of ~ 11 h this correlation decreased in 50%.
Fig. 9. (a) p53-CFP (green) in clonal MCF7+pU265+pU293 cells after 5-Gy γ-irradiation.
Time (in min) after irradiation is shown below images. (b) and (c) p53-CFP levels
(total CFP fluorescence in nuclei) from cells 1 and 2 (indicated by arrows in (a)),
showing the heterogeneity in the pulses generated after -irradiation. AU, arbitrary units.
Figure reproduced from Lahav et al. (2004), with authorization of Dr. U. Alon.
Geva-Zatorsky et al. (2006) work also reveals that after a treatment with 10Gy of -radiation,
40% of the cells showed a non-oscillatory response, or did not respond at all . The non-
oscillatory response consisted of slow fluctuations of Mdm2-YFP and p53-CFP with only
one, two or three peaks. Each fluctuation had duration of 8 – 12 h. Furthermore, a fraction of
non-irradiated cells also show this kind of fluctuations.
Dynamical Aspects of Apoptosis 261
After MCF7 cells division, p53 and Mdm2 oscillations continued with the same phase but
sister cells lose ~ 50% of correlation in their oscillatory dynamics after 11 h (Geva-Zatorski et
al., 2006). These results point to two important facts: 1) apoptotic information can be
transferred to daughter cells and 2) sister cells can inherit similar levels of relevant apoptotic
molecules, but noise generates fluctuations in protein production and degradation. As
consequence, synchrony is lost after a lapse of time (Spencer and Sorger, 2011). In this form,
the initial apoptotic timing synchronization caused by the .irradiation can be transferred to
daughter cells but this correlation falls as time from division increases, until sister cells are
no more correlated than a randomly chosen pair of cells (Spencer and Sorger, 2011).
start of apoptosis. In this model PUMA plays the role of accelerator of the Bax level
increasing process. The possible biological significance of this process may be to allow a lag
between the starting of the DNA damage repair process and the onset of full apoptosis
when the damage cannot be repaired in a pre-set moment of the cell cycle. After this time
lag, Puma reaches a threshold concentration value that fires the complete and irreversible
apoptotic process. From a dynamical point of view, this model states the stability and
robustness of the network of interaction between p53 and the pro and anti-apoptotic
proteins BclxL, Bcl2, Puma and Bax. The flow predicted by this model is formed by set of
trajectories that converge into an attractor in the 4-dimensional phase space spanned by the
concentration of the p53/Bcl-xL, PUMA/Bcl-xL, Bax/Bcl2 and p53/Bcl2 complexes. This
flow is reasonably independent of the values of the rate constants of association and
dissociation of these complexes, indicating its structural stability.
In this form, the cytoplasmic component of the apoptotic subnetwork exhibits a dynamics
driven by a saddle-node bifurcation, which settles down a control point that determine the
cell fate according to the information flow from the nucleus. This dynamics seems to be
started by a Bcl2 switch that initiates the accumulation of Bax in the cytoplasm, until it
reaches a threshold value. It seems that, although the cytoplasmic accumulation of Bax
initiates the liberation of cytochrome c from mitochondria in a time lapse as short as ~ 10
min, the process is still reversible. However, once PUMA is present in the cytoplasm, the
rate of liberation of Bax from the Bcl2/Bax complexes is sharply increased, and makes the
apoptotic process completely irreversible due to the consequent increase of the levels of Bax
over its threshold value. The high concentration of free Bax triggers the process of activation
of caspase 3.
5. Conclusions
Apoptosis is a complex molecular process, which is capable of determine the cell fate
according to the intensity of the DNA damage caused by IR (section 2.3). This network of
molecular processes has a highly connected node, which is protein p53. Its key role in
apoptosis is tightly regulated by a series of molecules like Wip1 and Mdm2, among others.
DSB´s and ss-DNA can trigger the activation of either ATM or ATR (section 2.4). These
kinases suppress the inhibitory activity of Mdm2 on p53, giving rise to a digital or to an
analog response to IR, respectively (section 4.1 and 4.2).
Cancer cells show abnormalities in their genetic network, which includes the regulatory
loops of apoptosis. As a result, the cancer cell response to IR is heterogeneous probably due
to three facts: 1) IR produces random damage to DNA (Ma et al., 2005; Qi et al., 2007); 2)
intrinsic noise adds some amount of randomness to the processes of transcription and
translation of key apoptotic proteins (Geva-Zatorsky et al., 2006; Díaz, 2011); 3) regulatory
genetic loops may be randomly disabled in cancer cells.
The effect of randomness is the generation of low frequency fluctuations in the levels of
proteins like Mdm2. When these fluctuations coincide with the natural frequency of oscillation
of the negative feedback loops that control p53 activation, the result is a series of pulses of
different amplitude (Geva-Zatorsky et al., 2006). However, the rhythm of the oscillations
remains relatively constant, suggesting that fluctuations principally affect the rate of synthesis
of proteins. Models that include this randomness are successful in reproducing the
heterogeneity of the pulses of p53 and Mdm2 in individual cells (Section 4.3).
However, there are not data about the oscillatory dynamics of p53 in normal cells after
perturbation with IR, neither in other lines of cancer cells. In this form, it is not known if the
264 Current Topics in Ionizing Radiation Research
6. Acknowledgments
We thank Erika Juarez Luna for technical and logistical assistance. Financial support for this
work was from CONACYT (Consejo Nacional de Ciencia y Tecnología) grant 105678.
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