PGS. TS.

Nguyn Ngc Hi - Khoa

CNTY - HNL Tp. HCM
Virus gy bnh dch t heo
(Classical Swine Fever Virus)
PGS. TS. Nguyn Ngc Hi - Khoa
CNTY - HNL Tp. HCM
T l bnh/ t l cht
T l bnh rt cao
T l cht
Th cp: 90 %
Th mn: cht hu ht
Th nh: cht t
Mt s trng hp khng biu hin
triu chng
PGS. TS. Nguyn Ngc Hi - Khoa
CNTY - HNL Tp. HCM
Ly nhim
Ly nhim cao
Thc n, nc ung
Tip xc heo bnh
Qua ng ming, h hp
Heo bnh = mm bnh
Trong dch tit, m
Khng ly nhim qua ngi
PGS. TS. Nguyn Ngc Hi - Khoa
CNTY - HNL Tp. HCM
Triu chng lm sng
bnh: 2-14 ngy
Thay i
Cp n khng c triu chng
Chng virus
Tnh mn cm ca vt ch

PGS. TS. Nguyn Ngc Hi - Khoa
CNTY - HNL Tp. HCM
By gi sau, virus ti sn u tin
hch hnh nhn
Mi su gi sau, virus xm nhp
vo cc hch bch huyt, h thng mch
mu ngoi vi gy nhim virus huyt
ln th nht
Mt lng ln virus c nhn ln
lch, tu xng, hch bch huyt ni
tng v ng tiu ho, cc bch cu
lu hnh v cc t bo n nhn
PGS. TS. Nguyn Ngc Hi - Khoa
CNTY - HNL Tp. HCM
Virus ph hu nhng t bo
ni mc mao mch nhng mnh
v s t li gy tc mch nhi
huyt lch, xut huyt v hoi t
rut nhim virus huyt ln hai
Virus DTH c c lc khp
cc t chc, biu m sau khi xm
nhp vo c th vt ch 5 6 ngy.
PGS. TS. Nguyn Ngc Hi - Khoa
CNTY - HNL Tp. HCM
PGS. TS. Nguyn Ngc Hi - Khoa
CNTY - HNL Tp. HCM
PGS. TS. Nguyn Ngc Hi - Khoa
CNTY - HNL Tp. HCM
Flaviviruses
Flaviviruses
Pestiviruses
Hepatitis C
Louping ill
Yellow fever
Dengue
Pestiviruses of Artydactyla
bovine viral diarrhoea
(BVDV)
hog cholera
(HCV)
border disease
(BDV)
Japanese
encephalitis
Murray Valley
St. Louis
encephalitis
West Nile
Wide host range (camelids, deer etc)
PGS. TS. Nguyn Ngc Hi - Khoa
CNTY - HNL Tp. HCM
Classical Swine Fever Virus
Lipid-enveloped RNA virus
c lc
Cp, di cp, mn, mang trng
Vt ch t nhin
Heo v heo rng
PGS. TS. Nguyn Ngc Hi - Khoa
CNTY - HNL Tp. HCM
Biotypes Pestivirus
CPE (-), (natural state)
CPE (+), (mutant)
PGS. TS. Nguyn Ngc Hi - Khoa
CNTY - HNL Tp. HCM
Pestiviruses
H Flaviviridae
Virus dch t heo (Hog Cholera Virus
HCV hoc Classical Swine Fever
CSF)
Tiu chy trn b (Bovin diarrhoea
Virus BVDV)
Border disease (BDV)
PGS. TS. Nguyn Ngc Hi - Khoa
CNTY - HNL Tp. HCM
PGS. TS. Nguyn Ngc Hi - Khoa
CNTY - HNL Tp. HCM
N
pro
C

E0

E1

E2

P7

NS2

NS3

NS4A

NS5A

NS4B

NS5B

Protein cu trc
Protein khng cu trc
(Non Structural Protein - NSP
Synsbiotic: p125
(NSP NS5B),
polymerase
Ceditest: E2
Envelope
PGS. TS. Nguyn Ngc Hi - Khoa
CNTY - HNL Tp. HCM
UTR
(Untranslated Region)
Structural
nonstructural
Vng c nhiu bin i nht
gp53
p125
(p54+p80)
PGS. TS. Nguyn Ngc Hi - Khoa
CNTY - HNL Tp. HCM
PGS. TS. Nguyn Ngc Hi - Khoa
CNTY - HNL Tp. HCM
PGS. TS. Nguyn Ngc Hi - Khoa
CNTY - HNL Tp. HCM
PGS. TS. Nguyn Ngc Hi - Khoa
CNTY - HNL Tp. HCM
PGS. TS. Nguyn Ngc Hi - Khoa
CNTY - HNL Tp. HCM
PGS. TS. Nguyn Ngc Hi - Khoa
CNTY - HNL Tp. HCM
PGS. TS. Nguyn Ngc Hi - Khoa
CNTY - HNL Tp. HCM
PGS. TS. Nguyn Ngc Hi - Khoa
CNTY - HNL Tp. HCM
PGS. TS. Nguyn Ngc Hi - Khoa
CNTY - HNL Tp. HCM
PGS. TS. Nguyn Ngc Hi - Khoa
CNTY - HNL Tp. HCM
PGS. TS. Nguyn Ngc Hi - Khoa
CNTY - HNL Tp. HCM
Chn on
Phng th nghim
Pht hin virus hoc khng nguyn
Mu mu khng ng hoc m
ELISA hoc direct
immunofluorescence
Huyt thanh hc
ELISA hoc trung ha virus
PGS. TS. Nguyn Ngc Hi - Khoa
CNTY - HNL Tp. HCM
Phng phap chan
oan
u iem
Nhc iem
K thut phan lp
virus (moi trng te
bao PK15)
Nhy hn phng phap khang
the hunh quang
Phat hin virus sm trong giai
on u ca bnh
Chm
K thuat khang the
hunh quang
Phat hin nhanh Khong nhy bng k
thut phan lp virus
K thut ELISA phat
hien khang nguyen
virus (E2)
Rat hiu qu trong vic chn
oan s lng ln heo b
nhim
Khong nhy bng k
thut phan lp virus
Cac phng phap chan oan
PGS. TS. Nguyn Ngc Hi - Khoa
CNTY - HNL Tp. HCM
Phng phap
u iem
Nhc iem
K thut
ELISA phat
hien khang
the (E2, p125)
Nhanh, n gin, rt hu hiu, quan trong
trong chan oan dch te
Phan biet khang the c tao ra do cm
nhim virus t nhien hay do vac-xin (p125), s
lng mu ln
Dng tnh gi (BVDV,
BDV)
Synsbiotic (p125)
Ceditest (E2)
K thut trung
hoa virus
Xac nh ham lng khang the Khong phan biet
khang the do cm
nhim virus t nhien hay
do vac-xin
K thut RT-
PCR
Nhanh, o nhy, c hiu cao,
Chn oan tien lam sang.
Dng tnh gi
Cac phng phap chan oan
PGS. TS. Nguyn Ngc Hi - Khoa
CNTY - HNL Tp. HCM
Mu (n = 12) ELISA pht hin P125 RT-PCR
1 + +
2 + +
3 + +
4 + +
5 - -
6 - +
7 + -
8 - +
CT-02 - -
CT-04 + +
CT-05 + -
CT-06 + +
Kt qu gia ELISA v RT-PCR
PGS. TS. Nguyn Ngc Hi - Khoa
CNTY - HNL Tp. HCM

Chan oan virus DTH tren te bao PK15
Am tnh Dng tnh
PGS. TS. Nguyn Ngc Hi - Khoa
CNTY - HNL Tp. HCM
PGS. TS. Nguyn Ngc Hi - Khoa
CNTY - HNL Tp. HCM
PGS. TS. Nguyn Ngc Hi - Khoa
CNTY - HNL Tp. HCM
Cc trinh t v v tr ca cc primer trong genome virus
PGS. TS. Nguyn Ngc Hi - Khoa
CNTY - HNL Tp. HCM
PGS. TS. Nguyn Ngc Hi - Khoa
CNTY - HNL Tp. HCM
PGS. TS. Nguyn Ngc Hi - Khoa
CNTY - HNL Tp. HCM
PGS. TS. Nguyn Ngc Hi - Khoa
CNTY - HNL Tp. HCM
PGS. TS. Nguyn Ngc Hi - Khoa
CNTY - HNL Tp. HCM
PGS. TS. Nguyn Ngc Hi - Khoa
CNTY - HNL Tp. HCM
c/ chuyn bit cua uniplex va multiplex
RT-PCR assays

PGS. TS. Nguyn Ngc Hi - Khoa
CNTY - HNL Tp. HCM
PGS. TS. Nguyn Ngc Hi - Khoa
CNTY - HNL Tp. HCM
PGS. TS. Nguyn Ngc Hi - Khoa
CNTY - HNL Tp. HCM
Phn b nhm di truyn CSFV Vit nam,
Thi lan, Trung quc, Malaysia v chu u
Nhm- Vit nam TQ Thi lan Malaysia chu u
gen 2002 88-95 96~ 80s ~ 1975 ~
O: c X: cha bo co
1.1
1.2
1.3
2.1
2.2
2.3
3.3
O
O
O
O
O
O
X
X
X
X
O
O
O
X

O
O
O
O
X
X
X
O
O
X
X
X
X
X
O
O
O
X
O
X
O
O
O
O
X
O
X
O
X
X
X
O
O
X
X
PGS. TS. Nguyn Ngc Hi - Khoa
CNTY - HNL Tp. HCM
1.1
1.2
1.3

2.1
2.2
2.3

3.3
Di nhm
PGS. TS. Nguyn Ngc Hi - Khoa
CNTY - HNL Tp. HCM
CSFV: Phn b di truyn SEA
1.1
1.3
2.1
3.1
3.2
3.3
2.3
2.2
1.2