Dale Akbar Yogaswara

J1C111015
pSC-A PCR Cloning Vector PCR Product Insertion Site Region
1. Sequence
ATGACCATGA TTACGCCAAG CGCGCAATTA ACCCTCACTA AAGGGAACAA
AAGCTGGGTA CCGGGCCCCC CCTCGAGGTC GACGGTATCG ATAAGCTTGA
TATCCACTGT GGAATTCGCC CTTAAGGGCG AATTCCACAT TGGGCTGCAG
CCCGGGGGAT CCACTAGTTC TAGAGCGGCC GCACCGCGGG AGCTCCAATT
CGCCCTATAG TGAGTCGTAT TACGCGCGCT CACTGGCCGT CGTTTTACAA
CGTCGTGACT GGGAAAACCC TGGCGTTACC CAACTTAATC GCCTTGCAGC
ACATCCCCCT TTCGCCAGCT GGCGTAATAG CGAAGAGGCC CGCACCGATT
AAATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA
AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG
GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT
GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT
ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG
AGACCCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG
GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG
TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG
TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT
CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT
ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC
GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG
CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT
GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG
ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA
GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA
CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG
TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT
GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA
CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT
TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA
AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT
TAATCGCCCT TCCCAACAGT TGCGCAGCCT GAATGGCGAA TGGGACGCGC
CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG
ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC
GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT
AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT
Dale Akbar Yogaswara
J1C111015
AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC
CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC
TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTACAGTTAA
TTAAAGGGAA CAAAAGCTGG CATGTACCGT TCGTATAGCA TACATTATAC
GAACGGTACG CTCCAATTCG CCCTTTAATT AACTGTTCCA ACTTTCACCA
TAATGAAATA AGATCACTAC CGGGCGTATT TTTTGAGTTG TCGAGATTTT
CAGGAGCTAA GGAAGCTAAA ATGGAGAAAA AAATCACTGG ATATACCACC
GAGTACTGCG ATGAGTGGCA GGGCGGGGCG TAATTTTTTT AAGGCAGTTA
TTGGTGCCCT TAAACGCCTG GTTGCTACGC CTGAATAAGT GATAATAAGC
GGATGAATGG CAGAAATTCG AAAGCAAATT CGACCCGGTC GTCGGTTCAG
GGCAGGGTCG TTAAATAGCC GCTTATGTCT ATTGCTGGTT TACCGGTTTA
TTGACTACCG GAAGCAGTGT GACCGTGTGC TTCTCAAATG CCTGAGGCCA
GTTTGCTCAG GCTCTCCCCG TGGAGGTAAT AATTGACGAT ATGATCCTTT TTTTCTGATC
AAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC
CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC
AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA
AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC
TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA
AATACTGTTC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC
TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG
CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG
TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA
GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG
AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT
CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG
GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC
TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA
AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT
TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT
TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT
CAGTGAGCGA GGAAGCGGAA GAGCGCCCAA TACGCAAACC GCCTCTCCCC
GCGCGTTGGC CGATTCATTA ATGCAGCTGG CACGACAGGT TTCCCGACTG
GAAAGCGGGC AGTGAGCGCA ACGCAATTAA TGTGAGTTAG CTCACTCATT
AGGCACCCCA GGCTTTACAC TTTATGCTCC CGGCTCGTAT GTTGTGTGGA
ATTGTGAGCG GATAACAATT TCACACAGGA AACAGCT

Dale Akbar Yogaswara
J1C111015
2. Features
Feature Nucleotide Position
-galactosidase -fragment (lacZ) 1352
T3 Primer 26-47
multiple cloning site (MCS) 57195
PCR product insertion site 123
ampicillin resistance (bla) ORF 4631320
f1 origin 15121818
<loxP> (nonfunctional) 18851918
pUC origin 24593126
lac promoter 33483467
M13 Reverse primer binding site 3458

3. Gene Mapping