Professional Documents
Culture Documents
• ZOONOSIS
• Vector-IXODID tick
• Resorvoir host-mice,voles and other
rodents
• Babesia microti-common cause of
babesiosis in US
Life cycle
• Infective stage-pyriform bodies
• Diagnostic stage-trophozoite
• In RBC-trophozoites by binary fission
forms tetrad-lyse erythrocytes-release
merozoites-reinfect
• Infected cells-ingested by ticks-
transovarian transmission
Clinical features
• DOC-Clindamycin+quinine
• Exchange blood transfusion in pts who
had splenenctomy and severe infections
• Protective clothing,insect repellents
TOXOPLASMA GONDII
• Coccidian parasite
• Reservoir host-house cat
• Infective stage-sporulated oocyst
• Diagnostic stage-immature oocyst in feces
• Some trophozoite forms-crescentic
tachyzoites-responsible for initial infection
• Slow growing shorter forms-bradyzoites-
cysts in chronic infection
• More severe CNS disease in
immunocompromised individuals
Clinical features
• Pyrimethamine+sulfadiazine high
dose,then continued in lower dose
indefenitely
• Trimethoprim-sulfamethoxazole is another
option
• Steroids-if cerebral oedema develops
• Prophylaxis-
sulfamethoxazole+trimethoprim
• Avoid contact with undercooked meat
FREE LIVING AMOEBA
• Naegleria
• Acanthamoeba
• Balamuthia
• Common route-Inhalation of cysts
Clinical features
• Specimens-Nasal discharge,CSF,corneal
scrapings
• Saline wet preparation and iodine stained
smears
• Naegleria-only amoeboid trophozoite;other
2-cyst and trophozoite
• Can be cultured on agar plates
Treatment, prevention and control
• Naegleria-amphotercin B +miconazole
and rifampin
• Acanthamoeba-
pentamidine,ketoconazole,flucytosine
• Balamuthia-
clarithromycin,fluconazole,sulfadiazine,fluc
ytosine
• Amoebic keratitis-corneal transplantation
• Cutaneous infections-topical
miconazole,chlorhexidine gluconate
LEISHMANIA
• Hemoflagellate
• Vector-sandfly-phlebotomus
• 3 species-
Leishmania donovani -visceral
leishmaniasis(kala-azar,dum dum fever)
L.Tropica -cutaneous
leishmaniasis(oriental sore,Delhi boil)
L.braziliensis -mucocutaneous
leishmaniasis(american
leishmaniasis,espundia,chiclero ulcer)
Leishmania donovani
Sandfly Transmission
• transmitted via mouthparts
• promastigotes regurgitated
from anterior gut
• factors in saliva enhance
infectivity
1) promastigotes
2) phagocytosis by
macrophage
amastigote
3) replication within
macrophage
4) release and
phagocytosis of
amastigotes
4) phagocytosis of
amastigotes, or
ingestion by
vector
5) promastigotes
• replication
• attachment to
epithelium
6) promastigotes
• Infective stage-promastigote
• Diagnostic stage-amastigote
• Resorvoir host-dogs,foxes,jackals
Clinical features
L. mexicana
Lab diagnosis
• DOC-stibogluconate
• Application of heat to the lesion
• Protection from bites
L.braziliensis
• T.gambiense-african
trypanosomiasis(sleeping sickness)
Vector-tsetse fly
• T.cruzi-chagas disease(american
trypanosomiasis)
Vector-reduvids
T.gambiense
• Infective stage-metacyclic trypomastigote
• Diagnostic stage-blood stream
trypomastigote
Clinical features
• Earliest-development of a
chagoma(erythematous and indurated
area)
• Followed by a rash and edema around
eyes and face
• Fever, chills and rigor
• CNS involvement
• c/c-
hepatosplenomegaly,myocarditis,enlargem
ent of oesophagus and colon
• Sudden death-heart block and brain
damage
Lab diagnosis
• DOC-NIFURTIMOX
• Also allopurinol
• Bug control, eradication of nests