SEPPIM S.A.S.

ZONE INDUSTRIELLE
61500 SEES - FRANCE
: 02 33 81 21 00 : 02 33 28 77 51









DIAGNOSTIC
REAGENTS

MANUAL METHODS





Version : MMAN-09 April 2010





SEPPIM S.A.S.
ZONE INDUSTRIELLE
61500 SEES - FRANCE
: 02 33 81 21 00 : 02 33 28 77 51




REAGENTS


A Version

ACID PHOSPHATASE .................................MM-PACI-1

ALBUMIN .................................................. MM-ALBU-1

ALP (DEA) SL .............................................. MM-PASL-1
ALP (DEA) ................................................... MM-PALC-1

ALT/GPT 4+1 SL .................................... MM-ALSL4/1-1
ALT/GPT ..................................................... MM-ALAT-2

AMYLASE SL ............................................. MM-AMSL-3

AST/GOT 4+1 SL .................................... MM-ASSL4/1-1
AST/GOT ..................................................... MM-ASAT-2



B
BILIRUBIN TOTAL & DIRECT 4+1 .......... MM-BITD-2



C
CALCIUM ARSENAZO ............................. MM-CALA-1
CALCIUM OCPC ........................................ MM-CALO-1

Update CHLORIDE ................................................. MM-CHLO-2

CHOLESTEROL SL ..................................... MM-CHSL-2
Update CHOLESTEROL ......................................... MM-CHOL-3
CHOLESTEROL HDL SL 2G .................... MM-HDLL-1
CHOLESTEROL LDL SL 2G ...................... MM-LDLL-1
HDL CHOLESTEROL ................................ MM-HDLC-2

CHOLINESTERASE .................................... MM-CHES-2

CK MB SL ................................................... MM-CMSL-2
CK MB ........................................................ MM-CKMB-2

CK NAC SL .................................................. MM-CKSL-2
CK NAC ....................................................... MM-CKNA-2

COPPER........................................................ MM-CUIV-1

CREATININE JAFFE .................................. MM-CRCO-1
CREATININE PAP SL ................................. MM-CRSL-3









G Version
GAMMA GT SL ........................................... MM-GASL-1
GAMMA GT ............................................... MM-GAGT-1

GLUCOSE HK SL ........................................ MM-GHSL-1
GLUCOSE PAP SL ...................................... MM-GPSL-2
GLUCOSE PAP ............................................ MM-GLUP-1


I

IRON CHROMAZUROL ............................. MM-FECA-1
IRON FERROZINE

................................... MM-FEFR-2


L
LACTATE .................................................... MM-LACT-2

New LDH-L SL ......................................................MM-LLSL-1
LDH-P .......................................................... MM-LDHP-2


M
MAGNESIUM CALMAGITE ................... MM-MAGN-1

MICROPROTEIN ........................................ MM-PRTP-2


P
PHOSPHORUS ............................................ MM-PHOS-1



T
TOTAL PROTEIN PLUS ............................. MM-PROB-1

TRIGLYCERIDES MONO SL NEW ...... MM-TGMLN-2
TRIGLYCERIDES ........................................ MM-TRIG-2


U
UREA UV SL ............................................... MM-URSL-2
UREA UV .................................................... MM-URUV-1

URIC ACID ................................................. MM-ACUR-2
URIC ACID MONO SL .............................. MM-AUML-2
Update URIC ACID SL ............................................ MM-AUSL-2

ELITECH ELITECH ELITECH




SEPPIM S.A.S.- ZONE INDUSTRIELLE - 61500 SEES - FRANCE


ACID
PHOSPHATASE






Ref. : PACI- 0030 18 x 3 mL



MANUAL METHOD




PROCEDURE

Wavelength : 405 nm
Temperature : 37C
Cuvette : 1 cm light path

Read against reagent blank.


Mix and after a 5 minute incubation measure the change of optical density per minute (OD/min.) during 3
minutes.


CALCULATION

Total ACP : Activity (U/L) = OD/min. x 853
NP ACP : Activity (U/L) = OD/min. x 860
Prostatic fraction (U/L) = Total ACP (U/L) - NP ACP (U/L).


LINEARITY

If OD/min. exceeds 0.070 at 405 nm, repeat test using serum diluted 1/10 with sodium chloride solution (9 g/L).
Multiply result by 10.


NOTE: For more details, see the Instructions for use
















(07/2005)
MM-PACI-1
Total acid phosphatase Non-prostatic acid phosphatase
Working Reagent 1 1 mL 1 mL
Working Reagent 2 10 L
Sample 100 L 100 L
ELITECH ELITECH ELITECH




SEPPIM S.A.S.- ZONE INDUSTRIELLE - 61500 SEES - FRANCE


ALBUMIN





Ref.: ALBU - 0600 2 x 125 mL
ALBU - 0700 4 x 250 mL

MANUAL METHOD




PROCEDURE

Wavelength : 628 nm
Temperature : 37C
Cuvette : 1 cm light path

Read against reagent blank.






Mix and read the optical density (OD) after a 5 minute incubation.
The final colour is stable for at least 15 minutes.


CALCULATION

OD Sample
x n
OD

Standard

n = standard concentration


NOTE: For more details, see the Instructions for use























(07/2005)
MM-ALBU-1
BLANK STANDARD SAMPLE
Reagent 1 mL 1 mL 1 mL
Distilled Water 10 L
Standard 10 L
Sample 10 L
ELITECH ELITECH ELITECH




SEPPIM S.A.S.- ZONE INDUSTRIELLE - 61500 SEES - FRANCE


ALP (DEA) SL





Rf. : PASL- 0400 2 x 62.5 mL
PASL- 0420 4 x 62.5 mL
PASL- 0500 5 x 125 mL



MANUAL METHOD




PROCEDURE

Wavelength : 405 nm (410)
Temperature : 37C, 30C, 25C
Cuvette : 1 cm light path
Read against distilled water.

One-reagent procedure



Mix and after a 1 minute incubation, measure the change of optical density per minute (OD/min.) during 3
minutes.

Two-reagent procedure



Mix, wait 1 minute and add:


Mix and after a 1 minute incubation, measure the change of optical density per minute (OD/min.) during 3
minutes.


CALCULATION
One reagent procedure Two reagents procedure
At 405 nm: Activity (U/L) = OD/min. x 2 750 3424
At 410 nm: Activity (U/L) = OD/min. x 2 910 3623


LINEARITY

If OD/min. exceeds 0.250 at 405 nm, repeat the test using serum diluted 1/10 with sodium chloride solution
(9 g/L). Multiply result by 10.


NOTE: For more details, see the Instructions for use










(07/2005)
MM-PASL-1
Working reagent : 1 mL
Sample : 20 L
R1 : 1 mL
Sample : 20 L
R2 : 250 L
ELITECH ELITECH ELITECH




SEPPIM S.A.S.- ZONE INDUSTRIELLE - 61500 SEES - FRANCE


ALP (DEA)





Rf. : PALC- 0030 20 x 3 mL
PALC- 0200 12 x 20 mL




MANUAL METHOD




PROCEDURE

Wavelength : 405 nm (410)
Temperature : 25C, 30C, 37C
Cuvette : 1 cm light path

Read against distilled water.



Mix and after a 1 minute incubation, measure the change of optical density per minute (OD/min.) during 3
minutes.


CALCULATION

At 405 nm: Activity (U/L) = OD/min. x 2 750
At 410 nm: Activity (U/L) = OD/min. x 2 910


LINEARITY

If OD/min. exceeds 0.250 at 405 nm, repeat the test using serum diluted 1/10 with sodium chloride solution (9
g/L). Multiply result by 10.


NOTE: For more details, see the Instructions for use

























(07/2005)
MM-PALC-1
Working reagent : 1 mL
Sample : 20 L
ELITECH ELITECH ELITECH




SEPPIM S.A.S.- ZONE INDUSTRIELLE - 61500 SEES - FRANCE


ALT/GPT 4+1 SL





Ref.: ALSL 0410 2 x 62.5 mL
ALSL 0430 4 x 62.5 mL
ALSL 0510 5 x 125 mL


MANUAL METHOD




PROCEDURE

Wavelength : 340 nm
Temperature : 37C
Cuvette : 1 cm light path
Read against distilled water.

One-reagent procedure



Mix and after a 1 minute incubation, measure the change of optical density per minute
(OD/min.) during 3 minutes.

Two-reagent procedure



Mix, wait 1 minute and add:


Mix and after a 1 minute incubation, measure the change of optical density per minute
(OD/min.) during 3 minutes.


CALCULATION

For the two procedures, at 340 nm: Activity (U/L) = OD/min. x 1 746


LINEARITY

If OD/min. exceeds 0.150 at 340 nm, repeat test using serum diluted 1/10 with sodium chloride solution (9 g/L).
Multiply result by 10.


NOTE: For more details, see the Instructions for use











(07/2005)
MM-ALSL4/1-1
Working reagent : 1 mL
Sample : 100 L
R1 : 1 mL
Sample : 125 L
R2 : 250 L
ELITECH ELITECH ELITECH




SEPPIM S.A.S.- ZONE INDUSTRIELLE - 61500 SEES - FRANCE


ALT/GPT





Ref.: ALAT - 0200 12 x 20 mL
ALAT - 0400 9 x 50 mL


MANUAL METHOD




PROCEDURE

Wavelength : 340 nm (334 365)
Temperature : 30C, 37C
Cuvette : 1 cm light path

Read against distilled water.



Mix and after a 1 minute incubation, measure the change of optical density per minute (OD/min.) during 3
minutes.


CALCULATION

At 340 nm: Activity (U/L) = OD/min. x 1 746
At 334 nm: Activity (U/L) = OD/min. x 1 780
At 365 nm: Activity (U/L) = OD/min. x 3235


LINEARITY

If OD/min. exceeds 0.150 at 340 nm, repeat test using serum diluted 1/10 with sodium chloride solution (9 g/L).
Multiply result by 10.


NOTE: For more details, see the Instructions for use






















(03/2008)
MM-ALAT-2
Working reagent : 1 mL
Sample : 100 L
ELITECH ELITECH ELITECH




SEPPIM S.A.S.- ZONE INDUSTRIELLE - 61500 SEES - FRANCE


AMYLASE SL





Rf. : AMSL - 0390 1 x 50 mL
AMSL - 0400 6 x 50 mL



MANUAL METHOD




PROCEDURE

Wavelength : 405 nm
Temperature : 30C, 37C
Cuvette : 1 cm light path

Read against distilled water.





Mix and after a 1 minute incubation, measure the change of optical density per minute (OD/min.) during 3
minutes.


CALCULATION
37 C 30 C
At 405 nm: Activity (U/L) = OD/min. x 4640 2812

Take dilution factor into account for the calculation of the activity in urine.


LINEARITY

Up to 900 U/L
If OD/min. exceeds 0.190 at 405 nm, repeat test using sample diluted 1/10 with sodium chloride solution
(9 g/L). Multiply result by 10.


NOTE: For more details, see the Instructions for use





















(11/2006)
MM-AMSL-3
37 C 30 C
Reagent 1 mL 1 mL
Sample 15 L 25 L
ELITECH ELITECH ELITECH




SEPPIM S.A.S.- ZONE INDUSTRIELLE - 61500 SEES - FRANCE


AST/GOT 4+1 SL





Rf. : ASSL- 0410 2 x 62.5 mL
ASSL- 0430 4 x 62.5 mL
ASSL- 0510 5 x 125 mL



MANUAL METHOD




PROCEDURE

Wavelength : 340 nm
Temperature : 37C
Cuvette : 1 cm light path
Read against distilled water.

One-reagent procedure



Mix and after a 1 minute incubation, measure the change of optical density per minute
(OD/min.) during 3 minutes.

Two-reagent procedure



Mix, wait 1 minute and add:


Mix and after a 1 minute incubation, measure the change of optical density per minute
(OD/min.) during 3 minutes.


CALCULATION

For the two procedures, at 340 nm: Activity (U/L) = OD/min. x 1 746


LINEARITY

If OD/min. exceeds 0.150 at 340 nm, repeat test using serum diluted 1/10 with sodium chloride solution (9 g/L).
Multiply result by 10.


NOTE: For more details, see the Instructions for use












(07/2005)
MM-ASSL4/1-1
Working reagent : 1 mL
Sample : 100 L
R1 : 1 mL
Sample : 125 L
R2 : 250 L
ELITECH ELITECH ELITECH




SEPPIM S.A.S.- ZONE INDUSTRIELLE - 61500 SEES - FRANCE


AST/GOT





Ref.: ASAT - 0200 12 x 20 mL
ASAT - 0400 9 x 50 mL


MANUAL METHOD




PROCEDURE

Wavelength : 340 nm (334 365)
Temperature : 30C, 37C
Cuvette : 1 cm light path

Read against distilled water.



Mix and after a 1 minute incubation, measure the change of optical density per minute (OD/min.) during 3
minutes.


CALCULATION

At 340 nm: Activity (U/L) = OD/min. x 1 746
At 334 nm: Activity (U/L) = OD/min. x 1 780
At 365 nm: Activity (U/L) = OD/min. x 3235


LINEARITY

If OD/min. exceeds 0.150 at 340 nm, repeat test using serum diluted 1/10 with sodium chloride solution (9 g/L).
Multiply result by 10.


NOTE: For more details, see the Instructions for use






















(07/2008)
MM-ASAT-2
Working reagent : 1 mL
Sample : 100 L
ELITECH ELITECH ELITECH




SEPPIM S.A.S.- ZONE INDUSTRIELLE - 61500 SEES - FRANCE
BILIRUBIN
TOTAL & DIRECT 4+1

Ref.: BITD 0600 Total 125 mL
Direct 125 mL
BITO - 0600 Total 2 x 125 mL
BIDI - 0600 Direct 2 x 125 mL




MANUAL METHOD



PROCEDURE for TOTAL BI LI RUBI N
Wavelength : 550 nm Temperature : 37C Cuvette : 1 cm light path

Read against distilled water.
CALIBRATION TEST
R1 BILIRUBIN TOTAL 4+1 800 L 800 L
Calibrator 100 L
Sample 100 L

Mix and read the optical density (OD) after 5 minutes of incubation at 37C
OD
1
OD
2


Then add:
R2 BILIRUBIN TOTAL & DIRECT 4+1 200 L 200 L

Mix and read the optical density (OD) after 5 minutes of incubation at 37C
OD
3
OD
4


PROCEDURE for DI RECT BI LI RUBI N
Wavelength : 550 nm Temperature : 37C Cuvette : 1 cm light path

Read against distilled water.
CALIBRATION TEST
R1 BILIRUBIN DIRECT 4+1 800 L 800 L
Calibrator 100 L
Sample 100 L

Mix and read the optical density (OD) after 5 minutes of incubation at 37C
OD
1
OD
2


Then add:
R2 BILIRUBIN TOTAL & DIRECT 4+1 200 L 200 L

Mix and read the optical density (OD) after 5 minutes of incubation at 37C
OD
3
OD
4


CALCULATI ON FOR BOTH PROCEDURES (Total and Direct Bilirubin)
With Factor: (OD
4
- OD
2
) x F

mg/dL: F = 14.0
mg/L: F = 140
mol/L: F = 240
With calibrator: OD
4
- OD
2
x n
OD
3
- OD
1


n = Calibrator concentration


NOTE: For more details, see the Instructions for use

(08/2009)
MM-BITD-2
ELITECH ELITECH ELITECH




SEPPIM S.A.S.- ZONE INDUSTRIELLE - 61500 SEES - FRANCE


CALCIUM
ARSENAZO





Ref.: CALA - 0600 2 x 125 mL


MANUAL METHOD




PROCEDURE

Wavelength : 650 nm (600)
Temperature : 37C
Cuvette : 1 cm light path

Read against distilled water.






Mix and read the optical density (OD) after a 1 minute incubation.
The final colour is stable for at least 1 hour.


CALCULATION

OD Sample
x n
OD

Standard

n = standard concentration

Take dilution factor into account for the calculation of the concentration in urine.


NOTE: For more details, see the Instructions for use


















(07/2005)
MM-CALA-1
BLANK STANDARD SAMPLE
Reagent 1 mL 1 mL 1 mL
Distilled Water 10 L
Standard 10 L
Sample 10 L
ELITECH ELITECH ELITECH




SEPPIM S.A.S.- ZONE INDUSTRIELLE - 61500 SEES - FRANCE


CALCIUM OCPC





Ref.: CALO - 0600 2 x 125 mL


MANUAL METHOD




PROCEDURE

Wavelength : 570 nm
Temperature : 37C
Cuvette : 1 cm light path

Read against distilled water.







Mix and read the optical density (OD) after a 5 minute incubation.
The final colour is stable for at least 1 hour.


CALCULATION

OD Sample
x n
OD

Standard

n = standard concentration

Take dilution factor into account for the calculation of the concentration in urine.


NOTE: For more details, see the Instructions for use




















(07/2005)
MM-CALO-1
BLANK STANDARD SAMPLE
Working Reagent 1 mL 1 mL 1 mL
Distilled Water 10 L
Standard 10 L
Sample 10 L
ELITECH ELITECH ELITECH


(09/2009)
MM-CHLO-2


SEPPIM S.A.S.- ZONE INDUSTRIELLE - 61500 SEES - FRANCE


CHLORIDE





Rf. : CHLO 0600 2 x 125 mL
250 mL



MANUAL METHOD



PROCEDURE

Wavelength : 480 nm
Temperature : 25C, 30C, 37C
Cuvette : 1 cm light path

Read against reagent blank.






Mix and read the optical density (OD) after a 5 minute incubation.
The final colour is stable for at least 1 hour.

I nformation for urine sample: The urine sample must be diluted with distilled water before analysis.

CALCULATION
Serum/Plasma: Urine:

n
Standard OD
Sample OD
2 n
Standard OD
Sample OD


n= Standard concentration


NOTE: For more details, see the Instructions for use
BLANK STANDARD SAMPLE
Reagent 1 mL 1 mL 1 mL
Distilled Water 10 L
Standard 10 L
Sample 10 L
ELITECH ELITECH ELITECH






SEPPIM S.A.S. ZONE INDUSTRIELLE - 61500 SEES - FRANCE


CHOLESTEROL SL





Rf. : CHSL- 0490 1 x 100 mL
CHSL- 0500 6 x 100 mL
CHSL- 0700 4 x 250 mL
CHSL- 0507 6 x 100 mL*
CHSL- 0707 4 x 250 mL*




MANUAL METHOD




PROCEDURE

Wavelength : 500 nm (492-550)
Temperature : 37C
Cuvette : 1 cm light path

Read against reagent blank.






Mix and read the optical density (OD) after a 5 minute incubation.
The final colour is stable for at least 1 hour.


CALCULATION

OD Sample x n
OD Standard

n= Standard concentration


NOTE: For more details, see the Instructions for use


















(07/2007)
* these kits contain a standard MM-CHSL-2
BLANK STANDARD SAMPLE
Reagent 1 mL 1 mL 1 mL
Distilled Water 10 L
Standard 10 L
Sample 10 L
ELITECH ELITECH ELITECH




SEPPIM S.A.S.- ZONE INDUSTRIELLE - 61500 SEES - FRANCE


CHOLESTEROL





Ref.: CHOL - 0220 12 x 20 mL
CHOL - 0420 9 x 50 mL





MANUAL METHOD



PROCEDURE

Wavelength : 500 nm (492 550)
Temperature : 37C
Cuvette : 1 cm light path

Read against reagent blank.






Mix and read the optical density (OD) after a 5 minute incubation.
The final colour is stable for at least 1 hour.


CALCULATI ON

OD Sample x n
OD Standard

n= Standard concentration


NOTE: For more details, see the Instructions for use























(03/2010)
MM-CHOL-3
BLANK STANDARD SAMPLE
Working Reagent 1 mL 1 mL 1 mL
Distilled Water 10 L
Standard 10 L
Sample 10 L
ELITECH ELITECH ELITECH




SEPPIM S.A.S.- ZONE INDUSTRIELLE - 61500 SEES - FRANCE


CHOLESTEROL
HDL SL 2G



Ref.: HDLL 0380 1 x 80 mL
HDLL 0390 3 x 80 mL





MANUAL METHOD




PROCEDURE

Wavelength : 600 nm
Temperature : 37C
Cuvette : 1 cm light path

Read against reagent blank.


BLANK STANDARD SAMPLE
R1 900 L 900 L 900 L
Distilled water 9 L
Standard 9 L
Sample 9 L

Mix and read absorbance (A1) after 5 minutes of incubation at 37C.

Then add:
R2 300 L 300 L 300 L

Mix and read absorbance (A2) after 5 minutes of incubation at 37C



CALCULATION

n
Calibrator Fdil A A
Sample Fdil A A

) (
) (
1 2
1 2
n = Calibrator concentration



Fdil = Dilution factor = (R1 volume + sample volume)/(R1 volume+R2 volume + sample volume)








NOTE: For more details, see the Instructions for use




(04/2009)
MM-HDLL-1
ELITECH ELITECH ELITECH




SEPPIM S.A.S.- ZONE INDUSTRIELLE - 61500 SEES - FRANCE


CHOLESTEROL
LDL SL 2G



Ref.: LDLL 0380 1 x 80 mL






MANUAL METHOD




PROCEDURE

Wavelength : 550 nm
Temperature : 37C
Cuvette : 1 cm light path

Read against reagent blank.


BLANK STANDARD SAMPLE
R1 900 L 900 L 900 L
Distilled water 9 L
Standard 9 L
Sample 9 L

Mix and read absorbance (A1) after 5 minutes of incubation at 37C.

Then add:
R2 300 L 300 L 300 L

Mix and read absorbance (A2) after 5 minutes of incubation at 37C



CALCULATION

n
Calibrator Fdil A A
Sample Fdil A A

) (
) (
1 2
1 2
n = Calibrator concentration



Fdil = Dilution factor = (R1 volume + sample volume)/(R1 volume+R2 volume + sample volume)








NOTE: For more details, see the Instructions for use




(04/2009)
MM-LDLL-1
ELITECH ELITECH ELITECH




SEPPIM S.A.S.- ZONE INDUSTRIELLE - 61500 SEES - FRANCE


HDL
CHOLESTEROL





Ref.: HDLC - 0060 3 x 10 mL x 100 mL


MANUAL METHOD



PROCEDURE

1) Sample preparation:
Add to 500 L of sample, 50 L of precipitating reagent. Mix, wait for 10 minutes and centrifuge at 5000 rpm
for 15 minutes. The supernatant is collected for HDL determination.

2) HDL determination:

Wavelength : 500 nm (492-550)
Temperature : 37C
Cuvette : 1 cm light path

Read against reagent blank.






Mix and read the optical density (OD) after a 5 minute incubation.
The final colour is stable for at least 1 hour.


CALCULATI ON

OD Sample x n x 1.1
OD Standard

n = Standard concentration
1.1 = Sample dilution factor


NOTE: For more details, see the Instructions for use














(09/2009)
MM-HDLC-2
BLANK STANDARD SAMPLE
Working Reagent 1 mL 1 mL 1 mL
Distilled Water 50 L
Standard 50 mg/dL 50 L
Supernatant 50 L
ELITECH ELITECH ELITECH




SEPPIM S.A.S.- ZONE INDUSTRIELLE - 61500 SEES - FRANCE


CHOLINESTERASE





Ref.: CHES - 0053 16 x 3 mL


MANUAL METHOD




PROCEDURE

Wavelength : 405 nm (400 440)
Temperature : 25C, 30C, 37C
Cuvette : 1 cm light path

Read against distilled water.




Mix and measure the change of optical density every 30 seconds (OD/30 s.) during 90 seconds.


CALCULATION

Activity (U/L) = OD/30 s. x 23 460


LINEARITY

If OD/30 sec. exceeds 0.200 at 405 nm, repeat test using serum diluted 1/5 with sodium chloride solution
(9 g/L). Multiply result by 10.


NOTE: For more details, see the Instructions for use



























(03/2008)
MM-CHES-2
Working reagent 1 : 3 mL
Sample : 20 L
Working reagent 2 : 100 L
ELITECH ELITECH ELITECH




SEPPIM S.A.S.- ZONE INDUSTRIELLE - 61500 SEES - FRANCE


CK MB SL





Rf. : CMSL- 0410 2 x 62.5 mL
CMSL- 0430 4 x 62.5 mL



MANUAL METHOD




PROCEDURE

Wavelength : 340 nm
Temperature : 30C, 37C
Cuvette : 1 cm light path

Read against distilled water.

One-reagent procedure



Mix and after a 3 minute incubation, measure the change of optical density per minute (OD/min.) during 3
minutes.

Two-reagent procedure



Mix and wait 30 seconds



Mix and after a 5 minute incubation, measure the change of optical density per minute (OD/min.) during 3
minutes.


CALCULATION

For the two procedures, at 340 nm: Activity (U/L) = OD/min. x 8254


LINEARITY

Inhibition : up to 2 000 U/L of CK-MM at 37 C.
If OD/min. exceeds 0.075 dilute the sample (one part with nine parts saline solution), repeat the test and
multiply the result by 10.
Linear up at least 600 U/L.


NOTE: For more details, see the Instructions for use





(11/2008)
: Modification from the previous version MM-CMSL-2

Working reagent : 1 mL
Sample : 40 L
R1 : 1 mL
R2 : 250 L
Sample : 50 L
ELITECH ELITECH ELITECH




SEPPIM S.A.S.- ZONE INDUSTRIELLE - 61500 SEES - FRANCE


CK MB





Rf. : CKMB- 0030 20 x 3 mL




MANUAL METHOD




PROCEDURE

Wavelength : 340 nm
Temperature : 30C, 37C
Cuvette : 1 cm light path

Read against distilled water.




Mix and after a 5 minute incubation, measure the change of optical density per minute (OD/min.) during 3
minutes.


CALCULATION

a) Total CK activity :
Determine total CK activity in serum using the CK-NAC reagent.

b) CK-B activity :
CK-B (U/L) = OD/min x 3 333

c) CK-MB activity :
CK-MB (U/L) = CK-B (U/L) x 2

d) Percentage of CK-MB activity in sample :
% CK-MB = CK-MB x 100
Total-CK


LINEARITY

Inhibition : up to 2000 U/L of CK-MM at 37 C.
If the assay of the total CK exceeds 1200 U/L at 37C, dilute the sample appropriately with physiological saline
before assay of CK-MB.
Multiply the result by the dilution factor to obtain the correct value of the isoenzyme.


NOTE: For more details, see the Instructions for use










(05/2006)
MM-CKMB-2
Working reagent : 1 mL
Sample : 50 L
ELITECH ELITECH ELITECH




SEPPIM S.A.S.- ZONE INDUSTRIELLE - 61500 SEES - FRANCE


CK NAC SL





Rf. : CKSL- 0410 2 x 62.5 mL
CKSL- 0430 4 x 62.5 mL



MANUAL METHOD




PROCEDURE

Wavelength : 340 nm
Temperature : 30C, 37C
Cuvette : 1 cm light path

Read against distilled water.

One-reagent procedure



Mix and after a 2 minute incubation, measure the change of optical density per minute (OD/min.) during 3
minutes.

Two-reagent procedure



Mix and wait 30 seconds



Mix and after a 2 minute incubation, measure the change of optical density per minute (OD/min.) during 3
minutes.


CALCULATION

For the two procedures, at 340 nm: Activity (U/L) = OD/min. x 4127


LINEARITY

If OD/min. exceeds 0.250 at 340 nm, repeat test using serum diluted 1/10 with sodium chloride solution (9 g/L).
Multiply result by 10.


NOTE: For more details, see the Instructions for use









(11/2008)
: Modification from the previous version MM-CKSL-2
Working reagent : 1 mL
Sample : 40 L
R1 : 1 mL
R2 : 250 L
Sample : 50 L
ELITECH ELITECH ELITECH




SEPPIM S.A.S.- ZONE INDUSTRIELLE - 61500 SEES - FRANCE


CK NAC





Rf. : CKNA- 0030 20 x 3 mL



MANUAL METHOD




PROCEDURE

Wavelength : 340 nm (334-365)
Temperature : 25C, 30C, 37C
Cuvette : 1 cm light path

Read against distilled water.




Mix and after a 2 minute incubation, measure the change of optical density per minute (OD/min.) during 3
minutes.


CALCULATION

At 340 nm : Activity (U/L) = OD/min. x 8 095
At 334 nm : Activity (U/L) = OD/min. x 8 252
At 365 nm : Activity (U/L) = OD/min. x 14 571


LINEARITY

If OD/min. exceeds 0.150 at 340 nm, repeat test using serum diluted 1/10 with sodium chloride solution (9 g/L).
Multiply result by 10.


NOTE: For more details, see the Instructions for use


























(03/2008)
MM-CKNA-2
Working reagent : 1 mL
Sample : 20 L
ELITECH ELITECH ELITECH




SEPPIM S.A.S.- ZONE INDUSTRIELLE - 61500 SEES - FRANCE


COPPER






Rf. : CUIV- 0050 5 x 10 mL



MANUAL METHOD




PROCEDURE

Wavelength : 580 nm (570-590)
Temperature : 37C
Cuvette : 1 cm light path

Read against reagent blank.

One-reagent procedure







Mix and read the optical density (OD) after a 5 minute incubation.

Two-reagent procedure







Mix and read the optical density (OD1).


Mix and read the optical density (OD2) after a 5 minute incubation.
The final colour is stable for at least 1 hour.


CALCULATION

One-reagent procedure :

OD Sample x n
OD Standard

Two-reagent procedure :

OD2 - OD1 Sample x n
OD2 - OD1 Standard
n = standard concentration


NOTE: For more details, see the Instructions for use





(07/2005)
MM-CUIV-1
BLANK STANDARD SAMPLE
Working Reagent 2 1 mL 1 mL 1 mL
Distilled Water 50 L
Standard 50 L
Sample 50 L
BLANK STANDARD SAMPLE
Working Reagent 1 1 mL 1 mL 1 mL
Distilled Water 50 L
Standard 50 L
Sample 50 L
Reagent 2 50 L 50 L 50 L
ELITECH ELITECH ELITECH




SEPPIM S.A.S.- ZONE INDUSTRIELLE - 61500 SEES - FRANCE


CREATININE
JAFFE





Ref.: CRCO - 0600 2 x 125 mL
CRCO - 0700 4 x 250 mL


MANUAL METHOD




PROCEDURE

Wavelength : 492 nm (480 520)
Temperature : 37C
Cuvette : 1 cm light path

Read against distilled water.




Mix and read the optical density (OD1) 10 seconds after the sample or standard addition. Exactly 2 minutes
after the first reading, take second reading (OD2).


CALCULATION

OD Sample x n
OD Standard

Take dilution factor into account for calculation of concentration in urine.


NOTE: For more details, see the Instructions for use



























(07/2005)
MM-CRCO-1
Working reagent : 1 mL
Sample : 100 L
ELITECH ELITECH ELITECH




SEPPIM S.A.S.- ZONE INDUSTRIELLE - 61500 SEES - FRANCE


CREATININE
PAP SL





Ref.: CRSL - 0630 2 x 133 mL



MANUAL METHOD




PROCEDURE

Wavelength : 550 nm
Temperature : 37C
Cuvette : 1 cm light path

Read against reagent blank.







Mix, and wait 5 minutes at 37C. Then, add:



Mix and read the change of optical density (OD) between 1 and 4 minutes..


CALCULATION

OD Sample
x n
OD

Standard

n = standard concentration

Take dilution factor into account for the calculation of the concentration in urine.


NOTE: For more details, see the Instructions for use












(03/2008)
MM-CRSL-3
BLANK STANDARD SAMPLE
R1 1.5 mL 1.5 mL 1.5 mL
Distilled Water 35 L
Standard 35 L
Sample 35 L
R2 0.5 mL 0.5 mL 0.5 mL
ELITECH ELITECH ELITECH




SEPPIM S.A.S.- ZONE INDUSTRIELLE - 61500 SEES - FRANCE


GAMMA GT SL





Ref.: GASL - 0400 2 x 62.5 mL
GASL - 0420 4 x 62.5 mL
GASL - 0500 5 x 125 mL


MANUAL METHOD




PROCEDURE

Wavelength : 405 nm
Temperature : 30C, 37C
Cuvette : 1 cm light path

Read against distilled water.

One-reagent procedure



Mix and after a 1 minute incubation, measure the change of optical density per minute
(OD/min.) during 3 minutes.

Two-reagent procedure



Mix, wait 1 minute and add:


Mix and after a 1 minute incubation, measure the change of optical density per minute
(OD/min.) during 3 minutes.


CALCULATION
One reagent procedure Two reagents procedure
At 405 nm: Activity (U/L) = OD/min. x 1 158 1421


LINEARITY
If OD/min. exceeds 0.200 at 405 nm, repeat test using serum diluted 1/10 with sodium chloride solution (9 g/L).
Multiply result by 10.


NOTE: For more details, see the Instructions for use












(07/2005)
MM-GASL-1
Working reagent : 1 mL
Sample : 100 L
R1 : 1 mL
Sample : 100 L
R2 : 250 L
ELITECH ELITECH ELITECH




SEPPIM S.A.S.- ZONE INDUSTRIELLE - 61500 SEES - FRANCE


GAMMA GT





Ref.: GAGT 0030 20 x 3 mL
GAGT 0200 12 x 20 mL


MANUAL METHOD




PROCEDURE

Wavelength : 405 nm
Temperature : 30C, 37C
Cuvette : 1 cm light path

Read against distilled water.



Mix and after a 1 minute incubation, measure the change of optical density per minute (OD/min.) during 3
minutes.


CALCULATION

At 405 nm: Activity (U/L) = OD/min. x 1 111


LINEARITY

If OD/min. exceeds 0.200 at 405 nm, repeat test using serum diluted 1/10 with sodium chloride solution (9 g/L).
Multiply result by 10.


NOTE: For more details, see the Instructions for use




























(07/2005)
MM-GAGT-1
Working reagent : 1 mL
Sample : 100 L
ELITECH ELITECH ELITECH




SEPPIM S.A.S.- ZONE INDUSTRIELLE - 61500 SEES - FRANCE


GLUCOSE HK SL





Ref.: GHSL - 0600 5 x 125 mL




MANUAL METHOD




PROCEDURE

Wavelength : 340 nm (334-365)
Temperature : 37C
Cuvette : 1 cm light path

Read against reagent blank.

One-reagent procedure







Mix and read the optical density (OD) after a 5 minute incubation.

Two-reagents procedure







Mix, wait 1 minute and add:


Mix and read the optical density (OD) after a 5 minute incubation.


CALCULATION

OD Sample x n
OD Standard

n= Standard concentration


NOTE: For more details, see the Instructions for use







(07/2005)
MM-GHSL-1
BLANK STANDARD SAMPLE
Working Reagent 1 mL 1 mL 1 mL
Distilled Water 10 L
Standard 10 L
Sample 10 L
BLANK STANDARD SAMPLE
R1 1 mL 1 mL 1 mL
Distilled Water 10 L
Standard 10 L
Sample 10 L
R2 250 L 250 L 250 L
ELITECH ELITECH ELITECH






SEPPIM S.A.S. ZONE INDUSTRIELLE - 61500 SEES - FRANCE


GLUCOSE PAP SL





Ref.: GPSL - 0490 1 x 100 mL
GPSL - 0500 6 x 100 mL
GPSL - 0700 4 x 250 mL
GPSL - 0507 6 x 100 mL*
GPSL - 0707 4 x 250 mL*




MANUAL METHOD




PROCEDURE

Wavelength : 500 nm (492-550)
Temperature : 37C
Cuvette : 1 cm light path

Read against reagent blank.






Mix and measure the optical density (OD) after a 10 minute incubation.
The reading can be done during 30 minutes.


CALCULATION

OD Sample x n
OD Standard

n= Standard concentration


NOTE: For more details, see the Instructions for use


















(07/2007)
* these kits contain a standard MM-GPSL-2
BLANK STANDARD SAMPLE
Working Reagent 1 mL 1 mL 1 mL
Distilled Water 10 L
Standard 10 L
Sample 10 L
ELITECH ELITECH ELITECH




SEPPIM S.A.S.- ZONE INDUSTRIELLE - 61500 SEES - FRANCE


GLUCOSE PAP





Ref.: GLUP - 0700 4 x 250 mL
GLUP - 0800 5 x 500 mL


MANUAL METHOD




PROCEDURE

Wavelength : 500 nm (492-550)
Temperature : 37C
Cuvette : 1 cm light path

Read against reagent blank.






Mix and read the optical density (OD) after a 10 minute incubation.
The final colour is stable for at least 1 hour.


CALCULATION

OD Sample x n
OD Standard

n= Standard concentration


NOTE: For more details, see the Instructions for use

























(07/2005)
MM-GLUP-1
BLANK STANDARD SAMPLE
Working Reagent 1 mL 1 mL 1 mL
Distilled Water 10 L
Standard 10 L
Sample 10 L
ELITECH ELITECH ELITECH




SEPPIM S.A.S.- ZONE INDUSTRIELLE - 61500 SEES - FRANCE


IRON
CHROMAZUROL





Ref.: FECA - 0600 2 x 125 mL


MANUAL METHOD




PROCEDURE

Wavelength : 637 nm
Temperature : 37C
Cuvette : 1 cm light path

Read against reagent blank.






Mix and read the optical density (OD) after a 15 minute incubation.
The final colour is stable for at least 1 hour.


CALCULATION

OD Sample x n
OD Standard

n= Standard concentration


NOTE: For more details, see the Instructions for use























(07/2005)
MM-FECA-1
BLANK STANDARD SAMPLE
Reagent 1 mL 1 mL 1 mL
Distilled Water 50 L
Standard 50 L
Sample 50 L
ELITECH ELITECH ELITECH




SEPPIM S.A.S.- ZONE INDUSTRIELLE - 61500 SEES - FRANCE


IRON FERROZINE





Ref.: FEFR 0600 2 x 125 mL


MANUAL METHOD



PROCEDURE

Wavelength : 560 nm (550-570)
Temperature : 37C
Cuvette : 1 cm light path
Read against distilled water.







Mix and read the optical density (OD).


Then add:


Mix and read the optical density (OD) within one hour.




CALCULATI ON

OD
4
- OD
2

x n
OD
3
- OD
1


n = standard concentration


NOTE: For more details, see the Instructions for use















(09/2009)
MM-FEFR-2
BLANK STANDARD SAMPLE
Working Reagent 1 mL 1 mL 1 mL
Distilled Water 200 L
Standard 200 L
Sample 200 L
- OD
1
OD
2

R2 50 L 50 L 50 L
- OD
3
OD
4

ELITECH ELITECH ELITECH




SEPPIM S.A.S.- ZONE INDUSTRIELLE - 61500 SEES - FRANCE


LACTATE





Rf. : LACT- 0100 10 x 10 mL




MANUAL METHOD




PROCEDURE

Wavelength : 540 nm (500-550)
Temperature : 37C
Cuvette : 1 cm light path

Read against reagent blank.






Mix and read the optical density (OD) after a 5 minute incubation.


CALCULATION

OD Sample x n
OD Standard

n= Standard concentration


NOTE: For more details, see the Instructions for use



























(05/2006)
MM-LACT-2
BLANK STANDARD SAMPLE
Working Reagent 1 mL 1 mL 1 mL
Distilled Water 10 L
Standard 10 L
Sample 10 L
ELITECH ELITECH ELITECH




SEPPIM S.A.S.- ZONE INDUSTRIELLE - 61500 SEES - FRANCE


LDH-L SL





Ref.: LLSL - 0400 2 x 62.5 mL
LLSL - 0420 4 x 62.5 mL





MANUAL METHOD



PROCEDURE

Wavelength : 340 nm
Temperature : 37C
Cuvette : 1 cm light path
Read against reagent blank.


Two-reagent procedure
BLANK SAMPLE
R1 1 mL 1 mL
Distilled Water 35 L
Sample 35 L

Mix, wait 3 minute and add:

R2 250 L 250 L



Mix and after a 100 second incubation, measure the change of optical density per minute
(OD/min.) during 3 minutes.


CALCULATI ON

At 340 nm: Activity (U/L) = OD/min. x 5828






NOTE: For more details, see the Instructions for use
















(10/2007)
MM-LLSL-1
ELITECH ELITECH ELITECH




SEPPIM S.A.S.- ZONE INDUSTRIELLE - 61500 SEES - FRANCE


LDH-P





Ref. : LDHP - 0030 20 x 3 mL




MANUAL METHOD




PROCEDURE

Wavelength : 340 nm (334-365)
Temperature : 30C, 37C
Cuvette : 1 cm light path

Read against distilled water.



Mix and after a 1 minute incubation, measure the change of optical density per minute (OD/min.) during 3
minutes.


CALCULATION

At 340 nm: Activity (U/L) = OD/min. x 8095
At 334 nm: Activity (U/L) = OD/min. x 8252
At 365 nm: Activity (U/L) = OD/min. x 15000


LINEARITY

If OD/min. exceeds 0.150 at 340 nm, repeat test using serum diluted 1/5 or 1/10 with sodium chloride solution
(9 g/L). Multiply result by 5 or 10.


NOTE: For more details, see the Instructions for use

























(03/2008)
MM-LDHP-2
Working reagent : 1 mL
Sample : 20 L
ELITECH ELITECH ELITECH




SEPPIM S.A.S.- ZONE INDUSTRIELLE - 61500 SEES - FRANCE


MAGNESIUM
CALMAGITE





Ref.: MAGN - 0600 2 x 125 mL


MANUAL METHOD




PROCEDURE

Wavelength : 520 nm (500-550)
Temperature : 37C
Cuvette : 1 cm light path

Read against reagent blank.






Mix and read the optical density (OD) after a 5 minute incubation.
The final colour is stable for at least 1 hour.


CALCULATION

OD Sample x n
OD Standard

n= Standard concentration

Take dilution factor into account for the calculation of magnesium concentration in urine.


NOTE: For more details, see the Instructions for use




















(07/2005)
MM-MAGN-1
BLANK STANDARD SAMPLE
Working Reagent 1 mL 1 mL 1 mL
Distilled Water 10 L
Standard 10 L
Sample 10 L
ELITECH ELITECH ELITECH




SEPPIM S.A.S.- ZONE INDUSTRIELLE - 61500 SEES - FRANCE


MICROPROTEIN





Ref.: PRTP - 0600 2 x 125 mL


MANUAL METHOD




PROCEDURE

Wavelength : 598 nm
Temperature : 37C
Cuvette : 1 cm light path

Read against reagent blank.






Mix and read the optical density (OD) after a 5 minute incubation.
The final colour is stable for 10 minutes.


CALCULATION

OD Sample x n
OD Standard

n= Standard concentration


NOTE: For more details, see the Instructions for use
























(11/2008)
: Modification from the previous version MM-PRTP-2
BLANK STANDARD SAMPLE
Reagent 1 mL 1 mL 1 mL
Distilled Water 20 L
Standard 20 L
Sample 20 L
ELITECH ELITECH ELITECH




SEPPIM S.A.S.- ZONE INDUSTRIELLE - 61500 SEES - FRANCE


PHOSPHORUS





Ref.: PHOS - 0600 2 x 125 mL


MANUAL METHOD




PROCEDURE

Wavelength : 340 nm
Temperature : 37C
Cuvette : 1 cm light path

Read against reagent blank.






Mix and read the optical density (OD) after a 5 minute incubation.
The final colour is stable for at least 1 hour.


CALCULATION

OD Sample x n
OD Standard

n= Standard concentration

Take dilution factor into account for the calculation of phosphorus concentration in urine.


NOTE: For more details, see the Instructions for use
























(07/2005)
MM-PHOS-1
BLANK STANDARD SAMPLE
Reagent 1 mL 1 mL 1 mL
Distilled Water 10 L
Standard 10 L
Sample 10 L
ELITECH ELITECH ELITECH
(08/2009)
MM-PROB-1


SEPPIM S.A.S.- ZONE INDUSTRIELLE - 61500 SEES - FRANCE


TOTAL PROTEIN PLUS


Rf.: PROB - 0600 2 x 125 mL
PROB - 0700 4 x 250 mL


MANUAL METHOD

PROCEDURE

Wavelength : 546 nm
Temperature : 37C
Cuvette : 1 cm light path

Read against reagent blank.







Mix and read the optical density (OD) after 10 minutes incubation.
The final colour is stable for 20 minutes.


CALCULATI ON

n
Standard OD
Sample OD



n= Standard concentration

NOTE: For more details, see the Instructions for use
BLANK STANDARD SAMPLE
Reagent 1 mL 1 mL 1 mL
Distilled Water 10 L
Standard 10 L
Sample 10 L
ELITECH ELITECH ELITECH






SEPPIM S.A.S. ZONE INDUSTRIELLE - 61500 SEES - FRANCE


TRIGLYCERIDES
MONO SL NEW





Rf. : TGML-0425 6 x 50 mL
TGML-0515 6 x 100 mL
TGML-0700 4 x 250 mL
TGML-0427 6 x 50 mL*
TGML-0517 6 x 100 mL*
TGML-0707 4 x 250 mL*





MANUAL METHOD




PROCEDURE

Wavelength : 500 nm
Temperature : 37C
Cuvette : 1 cm light path

Read against reagent blank.






Mix and read the optical density (OD) after a 10 minute incubation.


CALCULATION

OD Sample x n
OD Standard

n= Standard concentration


NOTE: For more details, see the Instructions for use
















(07/2007)
* these kits contain a standard MM-TGMLN-2
BLANK STANDARD SAMPLE
Reagent 1 mL 1 mL 1 mL
Distilled Water 10 L
Standard 10 L
Sample 10 L
ELITECH ELITECH ELITECH




SEPPIM S.A.S.- ZONE INDUSTRIELLE - 61500 SEES - FRANCE


TRIGLYCERIDES





Ref.: TRIG - 0200 12 x 20 mL
TRIG - 0400 9 x 50 mL





MANUAL METHOD




PROCEDURE

Wavelength : 546 nm (520 570)
Temperature : 37C
Cuvette : 1 cm light path

Read against reagent blank.






Mix and read the optical density (OD) after a 5 minute incubation.
The final colour is stable for at least 30 minutes.


CALCULATION

OD Sample x n
OD Standard

n= Standard concentration


NOTE: For more details, see the Instructions for use























(07/2007)
MM-TRIG-2
BLANK STANDARD SAMPLE
Working Reagent 1 mL 1 mL 1 mL
Distilled Water 10 L
Standard 10 L
Sample 10 L
ELITECH ELITECH ELITECH






SEPPIM S.A.S.- ZONE INDUSTRIELLE - 61500 SEES - FRANCE


UREA UV SL





Rf. : URSL- 0400 2 x 62.5 mL
URSL- 0420 4 x 62.5 mL
URSL- 0500 5 x 125 mL
URSL- 0407 2 x 62.5 mL*
URSL- 0427 4 x 62.5 mL*
URSL- 0507 5 x 125 mL*



MANUAL METHOD




PROCEDURE

Wavelength : 340 nm
Temperature : 37C
Cuvette : 1 cm light path
Read against distilled water.

One-reagent procedure



Mix and read the variation of optical density (OD) between 30 seconds and 90 seconds.

Two-reagent procedure




Mix, wait 1 minute and add:


Mix and read the variation of optical density (OD) between 30 seconds and 90 seconds.


CALCULATION

OD Sample x n
OD Standard

n = standard concentration


NOTE: For more details, see the Instructions for use









(07/2007)
* these kits contain a standard MM-URSL-2
Working reagent : 1 mL
Sample : 10 L
R1 : 1 mL
Sample : 10 L
R2 : 250 L
ELITECH ELITECH ELITECH




SEPPIM S.A.S.- ZONE INDUSTRIELLE - 61500 SEES - FRANCE


UREA UV





Rf. : URUV-0400 9 x 50 mL
URUV-0500 6 x 100 mL



MANUAL METHOD




PROCEDURE

Wavelength : 340 nm
Temperature : 37C, 30C
Cuvette : 1 cm light path

Read against reagent blank.






Mix and read the variation of optical density (OD) between 30 seconds and 90 seconds.


CALCULATION

OD Sample x n
OD Standard

n= Standard concentration

Take the dilution factor into account for the calculation of urea concentration in urine.


NOTE: For more details, see the Instructions for use

























(07/2005)
MM-URUV-1
BLANK STANDARD SAMPLE
Working Reagent 1 mL 1 mL 1 mL
Distilled Water 10 L
Standard 10 L
Sample 10 L
ELITECH ELITECH ELITECH




SEPPIM S.A..S- ZONE INDUSTRIELLE - 61500 SEES - FRANCE


URIC ACID





Rf. : ACUR - 0200 12 x 20 mL
ACUR - 0400 9 x 50 mL



MANUAL METHOD



PROCEDURE

Wavelength : 510 nm (492-550)
Temperature : 37C
Cuvette : 1 cm light path

Read against reagent blank.







Mix and read the optical density (OD) after a 5 minute incubation.
The final colour is stable for at least 15 minutes.


CALCULATI ON

OD Sample x n
OD Standard

n= Standard concentration

Take the dilution factor into account for the calculation of uric acid concentration in urine.


NOTE: For more details, see the Instructions for use





















(09/2009)
MM-ACUR-2
BLANK STANDARD SAMPLE
Working Reagent 1 mL 1 mL 1 mL
Distilled Water 25 L
Standard 25 L
Sample 25 L
ELITECH ELITECH ELITECH





SEPPIM S.A.S.- ZONE INDUSTRIELLE - 61500 SEES - FRANCE


URIC ACID
MONO SL





Rf. : AUML - 0420 6 x 50 mL
AUML - 0500 6 x 100 mL
AUML - 0700 4 x 250 mL
AUML - 0427 6 x 50 mL*
AUML - 0507 6 x 100 mL*
AUML - 0707 4 x 250 mL*




MANUAL METHOD




PROCEDURE

Wavelength : 555 nm
Temperature : 37C
Cuvette : 1 cm light path

Read against reagent blank.







Mix and read the optical density (OD) after a 5 minute incubation.


CALCULATION

OD Sample x n
OD Standard

n= Standard concentration

Take the dilution factor into account for the calculation of uric acid concentration in urine.


NOTE: For more details, see the Instructions for use















(07/2007)
* these kits contain a standard MM-AUML-2

BLANK STANDARD SAMPLE
Reagent 1 mL 1 mL 1 mL
Distilled Water 25 L
Standard 25 L
Sample 25 L
ELITECH ELITECH ELITECH




SEPPIM S.A.S.- ZONE INDUSTRIELLE - 61500 SEES - FRANCE


URIC ACID SL





Rf. : AUSL 0400 2 x 62.5 mL
AUSL 0600 5 x 125 mL



MANUAL METHOD



PROCEDURE

Wavelength : 555 nm
Temperature : 37C
Cuvette : 1 cm light path

Read against reagent blank.

One-reagent procedure







Mix and read the optical density (OD) after a 5 minute incubation.

Two-reagent procedure







Mix, wait 1 minute and add:


Mix and read the optical density (OD) after a 5 minute incubation.


CALCULATI ON

OD Sample x n
OD Standard

n= Standard concentration

Take the dilution factor into account for the calculation of uric acid concentration in urine.


NOTE: For more details, see the Instructions for use






(03/2010)
MM-AUSL-2
BLANK STANDARD SAMPLE
Working Reagent 1 mL 1 mL 1 mL
Distilled Water 25 L
Standard 25 L
Sample 25 L
BLANK STANDARD SAMPLE
R1 1 mL 1 mL 1 mL
Distilled Water 25 L
Standard 25 L
Sample 25 L
R2 250 L 250 L 250 L