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[S] (g/L)

[S] (mg/L)
1
1000
NOTA: el
5
5000
dato a 60g/L
10
10000
fue omitido
20
20000
ya que es un
30
30000
dato muy
40
40000
disperso.
50
50000
60
60000
70
70000
80
80000
PM de glucosa = 180 mg/mmol

[S] (mmol/L)
5.55555556
27.7777778
55.5555556
111.111111
166.666667
222.222222
277.777778
333.333333
388.888889
444.444444

Azucares reductores (mg/L)


Seobtuvieron con la curva de calibracion de la practica 1
X=y/m
m= 0.0005

D.O.
0
0
0
0.005
0
0.005
0.035
0.069
0.033
0.016

D.O.
0
0.002
0.005
0
0.023
0.012
0.018
0.071
0.038
0.024

Promedio
0
0.001
0.0025
0.0025
0.0115
0.0085
0.0265
0.07
0.0355
0.02

Dilucion

Azucares reductores (mg/L)


0
2
5
5
23
17
53
140
71
40

Azucares reductores (mmol/L)


0
0.011111111
0.027777778
0.027777778
0.127777778
0.094444444
0.294444444
0.777777778
0.394444444
0.222222222

Sacarosa Hidrolizada (mmol/L)


0
0.005555556
0.013888889
0.013888889
0.063888889
0.047222222
0.147222222
0.388888889
0.197222222
0.111111111

D.O.
1
2

Promedio
0.691
0.751

0.721

Dilucion

Concentracion (mg/L)
10.54093567

Concentracion (mg/L)
Se obtuvo con la curva de calibracion de proteina de la practica 1
y=mx
m= 0.0684

[S] (g/L)
1
5
10
20
30
40
50
70
80

[S] (mg/L)
1000
5000
10000
20000
30000
40000
50000
70000
80000

[S] (mmol/L) [S] (mmol/mg)


5.55555556
5.55556E-06
27.7777778
2.77778E-05
55.5555556
5.55556E-05
111.111111
0.000111111
166.666667
0.000166667
222.222222
0.000222222
277.777778
0.000277778
388.888889
0.000388889
444.444444
0.000444444

D.O.
0
0
0
0.005
0
0.005
0.035
0.033
0.016

D.O.
0
0.002
0.005
0
0.023
0.012
0.018
0.038
0.024

[S] inicial

Vel. Hidrolisis (mmol/mg min)

Cinetica de la invertasa
0.0009
0.0008
0.0007
0.0006
0.0005
0.0004
0.0003
0.0002
0.0001
0
0

100

200

300

[S] (mmol/L)

400

500

Promedio
0
0.001
0.0025
0.0025
0.0115
0.0085
0.0265
0.0355
0.02

Azucares reductores (mg/L)


0
2
5
5
23
17
53
71
40

Azucares reductores (mmol/L)


0
0.011111111
0.027777778
0.027777778
0.127777778
0.094444444
0.294444444
0.394444444
0.222222222

Sacarosa Hidrolizada (mmol/L)


0
0.005555556
0.013888889
0.013888889
0.063888889
0.047222222
0.147222222
0.197222222
0.111111111

DS(mmol/L)

1/Vel (mg min/mmol)

Lineweaber Burk

y = 1E+06x - 960.97
R = 0.9468

50000
45000
40000
35000
30000
25000
20000
15000
10000
5000
0
0

0.005

0.01

0.015

0.02
1/S (L/mmol)

0.025

0.03

0.035

0.04

Vel. Hidrolisis (UII)


mmol de sac.Hidro/L min
0
0.00037037
0.000925926
0.000925926
0.004259259
0.003148148
0.009814815
0.013148148
0.007407407

Vel. Hidrolisis (UIEI)


mmol de sac.Hidro/mg de prot * min
0
2.31481E-05
5.78704E-05
5.78704E-05
0.000266204
0.000196759
0.000613426
0.000821759
0.000462963

Eadie-Hofstee
0.001

% Sustrato consumido
0
0.04
0.05
0.025
0.076666667
0.0425
0.106
0.101428571
0.05

y = 345.71x - 0.0001
R = 0.7221

2500000
2000000
[S]/Vel. (min)

V (mmol/mg min)

0.0008
0.0006
0.0004
0.0002

1000000
500000

0
0
-0.0002

1500000

0.0000005

0.000001

0.0000015

Vel./[S] (1/min)

0.000002

0.0000025

1/[S]
0.18
0.036
0.018
0.009
0.006
0.0045
0.0036
0.002571429
0.00225

1/Vel.
0
43200
17280
17280
3756.521739
5082.352941
1630.188679
1216.901408
2160

Vel./[S]
0
8.3333E-07
1.0417E-06
5.2083E-07
1.5972E-06
8.8542E-07
2.2083E-06
2.1131E-06
1.0417E-06

Hanes-wooff

[S]/Vel.
0
1200000
960000
1920000
626086.957
1129411.76
452830.189
473239.437
960000

y = -3E+08x + 921515
R = 0.0092

2500000
2000000
1500000
1000000
500000
0
0

0.0001

0.0002

0.0003

[S] (mmol/L)

0.0004

0.0005

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