Notes on Chemistry Colloquium

The effect of microenvironment on cell behavior and tissue functionNanoscopic Investigations (Rong
Wang)
Department of Biological and chemical Sciences
Microenvironment: what it does, Impact of microenvironment on protein and cell functions: new
methods and new materials
1-Single cell Manipulation
The environment is very important and are not always by themselves.
The cell exist as a cluster
Differentiation
Quantitative spatiotemporal single cell level.
Stem cell heterogeneity and fate decision
Best situation into forming the phase of differentiation and hopefully becomes a neuron cell.
Scheme of Atomic Force Microscope:
Photodiode
Deflection signal
Inorganic System
Atomic alignment crystalline structure
Organic System
Molecular assembly phase separation correlation of species
Atomi Force Microscope (AFM)
A surface tool on the nano scale
Biological Systems
Scheme of two-tier AFM for protein delivery
Protein distribution. Association and local abundance on a cell membrane
Photolabile Cross Linker
Scheme of two tier
Identification of binding partner
Mrna retrieval for in situ seingle cell gene expression analysis
Wanting to make compatability with biomaterials, therefore they use collagen: the natural
polymer
Combination of collagen and silk protein and transplanted into the spider-goat- produces spider silk
proteins in milk.
Collagen silk composite fibers are mechanically stronger more stable in cell culture condition
and provide excellent cell adhesion to promote stem cell proliferation and neural differentiation