Gomes Lab/University of California, Davis

Aldrin/Gomes Lab Oct 2010

10X SDS-PAGE Running buffer

Components 1L 4L Final Concentration
Tris base 30.2 g 120.8g 250 mM Tris
Glycine 144 g 576g 1.92 M glycine
SDS 10 g 40g 1% SDS
make to 1L 4L with dH
2
O

Components
: Reagent
Source Catalog or
Protocol No.
F.W. or Stock
Conc.

Tris base Fisher Biotech BP152-5 121

Glycine Fisher Biotech BP381-5 75

Sodium dodecyl
sulfate (SDS)
Fisher Biotech BP166-500 288


Add Tris, then Glycine and make up the solution to close to the desired final volume. Then add SDS and
stir for 10 minutes. Make up the volume of the solution to the desired volume (1L or 4L) and stir for 2-3
minutes.
SDS is a detergent. Handle it with care and take preventative measures to prevent the SDS from going
into the air.
Check the pH of the solution. The pH of the buffer should be between 8.1-8.5. DO NOT ADJUST PH
WITH ACID OR BASE!!!